IJAR.2025.201
Type of Article: Original Research
Volume 13; Issue 3 (September 2025)
Page No.: 9306-9313
DOI: https://dx.doi.org/10.16965/ijar.2025.201
Automated Classification of Normal Histological Tissues Using Convolution neural network: A ResNet-50-Based Educational Tool
Haripriya 1, Sharad Gavhane 2, K.Vijayakumar *3.
1 Associate Professor, Department of Anatomy, Sri Ramachandra Medical College & Research Institute (SRMC & RI), Sri Ramachandra Institute for Higher Education and Research (SRIHER), Chennai, Tamil Nadu, India. ORCiD: https://orcid.org/0000-0001-6136-7576
2 Medical officer Department of General Medicine, Symbiosis Medical College and hospital (SUHRC), Symbiosis International (Deemed) University (SIU) Pune, Maharashtra, India. ORCiD: https://orcid.org/0009-0001-6627-4560
*3 Assistant Professor, Department of Anatomy, Symbiosis Medical College for Women & Symbiosis University Hospital and Research Centre, Symbiosis International (Deemed University), Pune, India. ORCiD: https://orcid.org/0000-0003-3032-8974
Corresponding Author: Dr. K. Vijayakumar, Assistant Professor, Department of Anatomy, Symbiosis Medical College for Women & Symbiosis University Hospital and Research Centre, Symbiosis International (Deemed University), Pune, India. Mobile: 9940695046 E-Mail: kvijay.india@gmail.com
ABSTRACT
Background: The process of identifying histological slides is an essential part of medical education and pathology. AI tools can perform a decent job in radiology and dermatopathology, but the gap for fully automated, multi-class histological tissue classification is significant for teaching and learning purposes.
Aim: To create and validate a deep learning system developed on 13 categories of normal human fine tissue using Convolutional Neural Networks (CNN) to aid in extended histology education and non-diagnostic digital pathology.
Methods: This is a developmental diagnostic accuracy study with an experimental computational design. A total of 3250 high-resolution, expert-annotated images depicting 13 different normal histological tissue types. Training (70%), validation (15%), and testing (15%) sets were split from the dataset. The remaining methods fine-tuned three CNN architectures: ResNet-50, EfficientNet-B0, and MobileNetV2.
Results: The performance results ranking for ResNet-50 was better overall among the three models, with an overall accuracy, precision, recall, and F1-score of 94. 2%, 93. 8%, 93. 5%, and 93. 6% in the test set, respectively. The accuracies of EfficientNet-B0 and MobileNetV2 were 91.7% and 89.3%, respectively.
Conclusion: The proposed ResNet-50-based CNN model has high accuracy in diagnosing normal histological tissues and can be a useful training tool for medical and paramedical students. This study fills a crucial void by offering an automated, scalable, and explainable system for histological image classification, with a high potential for integration into digital learning devices.
Keywords: Histology, Deep Learning, ResNet-50, Convolutional Neural Networks, Digital Pathology, Medical Education.
REFERENCES
[1]. Ball CS. The Early History of the Compound Microscope. Bios. 1966;37(2):51-60.
[2]. Wills M. The Evolution of the Microscope [Internet]. JSTOR Daily. 2018.
[3]. Pallua JD, Brunner A, Zelger B, Schirmer M, Haybaeck J. The future of pathology is digital. Pathol Res Pract. 2020 Sep;216(9):153040.
https://doi.org/10.1016/j.prp.2020.153040
PMid:32825928
[4]. Al-Janabi S, Huisman A, Van Diest PJ. Digital pathology: current status and future perspectives. Histopathology. 2012;61(1):1-9.
https://doi.org/10.1111/j.1365-2559.2011.03814.x
PMid:21477260
[5]. Ahmed AA, Abouzid M, Kaczmarek E. Deep Learning Approaches in Histopathology. Cancers. 2022 Oct 26;14(21):5264.
https://doi.org/10.3390/cancers14215264
PMid:36358683 PMCid:PMC9654172
[6]. El-Sherif DM, Abouzid M, Elzarif MT, Ahmed AA, Albakri A, Alshehri MM. Telehealth and Artificial Intelligence Insights into Healthcare during the COVID-19 Pandemic. Healthcare. 2022 Feb 18;10(2):385.
https://doi.org/10.3390/healthcare10020385
PMid:35206998 PMCid:PMC8871559
[7]. Fuchs TJ, Buhmann JM. Computational pathology: challenges and promises for tissue analysis. Comput Med Imaging Graph. 2011;35(7-8):515-30.
https://doi.org/10.1016/j.compmedimag.2011.02.006
PMid:21481567
[8]. Louis DN, Feldman M, Carter AB, Dighe AS, Pfeifer JD, Bry L, et al. Computational Pathology. Arch Pathol Lab Med. 2016 Jan;140(1):41-50.
https://doi.org/10.5858/arpa.2015-0093-SA
PMid:26098131 PMCid:PMC4996078
[9]. Kulikowski CA, Shortliffe EH, Currie LM, Elkin PL, Hunter LE, Johnson TR, et al. AMIA Board white paper: definition of biomedical informatics and specification of core competencies for graduate education in the discipline. J Am Med Inform Assoc. 2012;19(6):931-8.
https://doi.org/10.1136/amiajnl-2012-001053
PMid:22683918 PMCid:PMC3534470
[10]. Prewitt JMS, Mendelsohn ML. The Analysis Of Cell Images. Annals of the New York Academy of Sciences. 2006 ;128(3):1035-53.
https://doi.org/10.1111/j.1749-6632.1965.tb11715.x
PMid:5220765
[11]. Zhang Y, Ouyang Z, Zhao H. A statistical framework for data integration through graphical models with application to cancer genomics. The Annals of Applied Statistics. 2017 Mar 1;11(1).
https://doi.org/10.1214/16-AOAS998
[12]. Li W, Li J, Sarma KV, Ho KC, Shen S, Knudsen BS, et al. Path R-CNN for Prostate Cancer Diagnosis and Gleason Grading of Histological Images. IEEE Transactions on Medical Imaging. 2019 Apr;38(4):945-54.14.
https://doi.org/10.1109/TMI.2018.2875868
PMid:30334752 PMCid:PMC6497079
[13]. LeCun Y, Bagnio Y, Hinton G. Deep Learning. Nature. 2015 May;521(7553):436-44.
https://doi.org/10.1038/nature14539
PMid:26017442
[14]. Syrykh C, Abreu A, Amara N, Siegfried A, Maisongrosse V, Frenois FX, et al. Accurate diagnosis of lymphoma on whole-slide histopathology images using deep learning. npj Digital Medicine. 2020 May 1;3(1).
https://doi.org/10.1038/s41746-020-0272-0
PMid:32377574 PMCid:PMC7195401
[15]. Esteva A, Chou K, Yeung S, Naik N, Madani A, Mottaghi A, et al. Deep learning-enabled medical computer vision. npj Digit Med. 2021 Jan 8;4(1):1-9.
https://doi.org/10.1038/s41746-020-00376-2
PMid:33420381 PMCid:PMC7794558
[16]. Coudray N, Ocampo PS, Sakellaropoulos T, Narula N, Snuderl M, Fenyö D, et al. Classification and mutation prediction from non-small cell lung cancer histopathology images using deep learning. Nat Med. 2018 Oct;24(10):1559-67.
https://doi.org/10.1038/s41591-018-0177-5
PMid:30224757 PMCid:PMC9847512
[17]. Dercle L, McGale J, Sun S, Marabelle A, Yeh R, Deutsch E, et al. Artificial intelligence and radiomics: fundamentals, applications, and challenges in immunotherapy. J Immunother Cancer. 2022 Sep;10(9):e005292.
https://doi.org/10.1136/jitc-2022-005292
PMid:36180071 PMCid:PMC9528623
[18]. Campanella G, Hanna MG, Geneslaw L, Miraflor A, Werneck Krauss Silva V, Busam KJ, et al. Clinical-grade computational pathology using weakly supervised deep learning on whole slide images. Nature Medicine [Internet]. 2019 Aug 1;25(8):1301-9.
https://doi.org/10.1038/s41591-019-0508-1
PMid:31308507 PMCid:PMC7418463
[19]. Madani A, Ong JR, Tibrewal A, Mofrad MRK. Deep echocardiography: data-efficient supervised and semi-supervised deep learning towards automated diagnosis of cardiac disease. NPJ Digit Med. 2018;1:59.
https://doi.org/10.1038/s41746-018-0065-x
PMid:31304338 PMCid:PMC6550282








