Type of Article:  Original Research

Volume 11; Issue 4 (December 2023)

Page No.: 8778-8783

DOI: https://dx.doi.org/10.16965/ijar.2023.222

Co-Supplementation of Vitamin E & Nigella sativa Improved the Endometrial Thickness in Mice

Sabrina Muhamad Jalani1, Nurul Hannah Jaafar2, Ruzianisra Mohamed3, Siti Syairah Mohd Mutalip*4.

1 Faculty of Pharmacy, Universiti Teknologi MARA (UiTM) Puncak Alam Campus, Selangor, Malaysia.

2 Faculty of Pharmacy, Universiti Teknologi MARA (UiTM) Puncak Alam Campus, Selangor, Malaysia.

3 Faculty of Pharmacy, Universiti Teknologi MARA (UiTM) Puncak Alam Campus, Selangor, Malaysia. ORCiD: 0000-0003-2398-4164

*4 Faculty of Pharmacy, Universiti Teknologi MARA (UiTM) Puncak Alam Campus, Selangor, Malaysia. ORCiD: 0000-0002-4104-518X

Corresponding Author: Siti Syairah Mohd Mutalip, Faculty of Pharmacy, Universiti Teknologi MARA (UiTM) Puncak Alam Campus, Selangor, Malaysia. E-Mail: syairah@uitm.edu.my


Background: The thin endometrial lining has been reported to be associated with significant complications in maintaining a healthy pregnancy. Studies have been conducted to find ways to improve this condition, and one of the possibilities is through micronutrient intake. Vitamin E and Nigella sativa (NS) are the two potential sources of micronutrients that could play essential roles in improving endometrial thickness.

Purpose: The present study was conducted to determine the effect of vitamin E and NS supplementation on endometrial thickness in normal (not induced to any disease) mice.

Methods: Twenty-four balb/c mice were divided into four groups with six mice each; Group A as negative control (no treatment), Group B given with vitamin E (400 mg/kg), Group C given with NS (200 mg/kg) and Group D given with both vitamin E (400 mg/kg) and NS (200 mg/kg). The uterus histological sections from each group were evaluated for endometrial thickness and other structures of the uterus components were also observed.

Results: Co-supplementation of vitamin E and NS have a significantly higher endometrial thickness (131.84 + 14.48μm) compared to the control group (88.08 + 11.49μm) and other uterus structures were not disrupted.

Conclusion: The co-supplementation of vitamin E and NS could enhance the endometrial thickness without inducing any disruption to the other structures of the uterus.

Keywords: Endometrial thickness; vitamin E; Nigella sativa; uterus histology.


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Cite this article: Sabrina Muhamad Jalani, Nurul Hannah Jaafar, Ruzianisra Mohamed, Siti Syairah Mohd Mutalip. Co-Supplementation of Vitamin E & Nigella sativa Improved the Endometrial Thickness in Mice. Int J Anat Res 2023;11(4):8778-8783. DOI: 10.16965/ijar.2023.222