Type of Article:  Original Research

Volume 11; Issue 4 (December 2023)

Page No.: 8740-8747

DOI: https://dx.doi.org/10.16965/ijar.2023.204

Protective Effect of Centella asiatica on AlCl3 and D-Galactose Induced Hepatotoxicity in Rats through the Alleviation of Oxidative Stress as Demonstrated by Histological Changes in Liver

Thirupathirao. Vishnumukkala 1, 2, Prarthana Kalerammana Gopalakrishna 3, Barani Karikalan 4 , Saravanan Jagadeesan 5, Mohamad Taufik Hidayat B. Baharuldin6, Warren Thomas7, Mohamad Aris Mohd Moklas *8.

1 Lecturer, Department of Anatomy, Human Biology Division, School of Medicine, International Medical University, Kuala Lumpur, Malaysia. ORCiD: 0000-0002-9517-3726

2 Ph.D candidate, Department of Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.

3 Lecturer, Department of Physiology, Human Biology division, School of Medicine, International Medical University, Kuala Lumpur, Malaysia. ORCiD: 0000-0002-1428-5305

4 Associate Professor, Department of Pathology, Faculty of Medicine, Bioscience and Nursing, MAHSA university, Bandar Saujana Putra, Selangor, Malaysia. ORCiD: 0000-0002-5751-346X

5 Associate Professor, Department of Anatomy, School of Medicine, Taylors University, Lakeside Campus, Selangor, Malaysia. ORCiD: 0000-0001-7389-7363

6 Unit of Physiology, Dept.of Preclinical, Faculty of Medicine and Defence Health, Universiti Pertahanan Nasional Malaysia, Kuala Lumpur, Malaysia. ORCiD: 0000-0003-4773-8531

7 Associate Professor, Department of Human Biology, Royal College of Surgeons in Ireland – Medical University of Bahrain, Al Sayh, Muharraq Governate, Kingdom of Bahrain. ORCiD: 0000-0003-2706-1770

8 Associate Professor, Department of Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia. ORCiD: 0000-0002-9282-129X.

Corresponding author: Assoc. Prof. Dr. Mohamad Aris Bin Mohd Moklas. PhD (Nottingham University), Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Selangor, Malaysia. +603-97692783 E-Mail: aris@upm.edu.my


Background: Injury to the liver resulting from drug exposure is known as hepatotoxicity. Drug-induced liver damage is a clinical consequence that can be challenging to recognise, avoid, and manage. After administering Centella asiatica extract to rats that have hepatotoxicity induced by AlCl3 and D-galactose, we evaluated the histopathological alterations in the hepatic tissue and measured the concentration of oxidative and anti-oxidative activity.

Objective: The objective of this study is to investigate the protective benefits of Centella asiatica extract on AlCl3 and D-galactose-induced hepatotoxicity.

Materials and methods:  Male albino Wistar rats were used in this study. Centella asiatica extract (100mg, 200mg and 300mg/kg/day) was given orally to the AlCl3 and D-gal induced hepatotoxic rats for seventy days. At the end of treatment, the liver was harvested and the activity levels of oxidative and antioxidative enzymes were determined.  Histopathological changes in the liver were also documented.

Results: Centella asiatica extract significantly raised the levels of SOD and catalase in liver homogenates, while lowering MDA levels and suppressing histopathological changes such as bridging necrosis, intralobular degeneration, focal necrosis, and fibrosis alterations in the liver.

Conclusion: Centella asiatica extract reduced oxidative stress levels in drug-induced hepatotoxicity by attenuating histological changes and normalising the levels of oxidative stress indicators in the liver.

Keywords: Centella asiatica, Hepatotoxicity, AlCl3, D-galactose, Oxidative stress.


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Cite this article: Thirupathirao. Vishnumukkala, Prarthana Kalerammana Gopalakrishna, Barani Karikalan, Saravanan Jagadeesan, Mohamad Taufik Hidayat B. Baharuldin, Warren Thomas, Mohamad Aris Mohd Moklas. Protective Effect of Centella asiatica on AlCl3 and D-Galactose Induced Hepatotoxicity in Rats through the Alleviation of Oxidative Stress as Demonstrated by Histological Changes in Liver. Int J Anat Res 2023;11(4):8740-8747. DOI: 10.16965/ijar.2023.204