Type of Article:  Original Research

Volume 11; Issue 2 (June2023)

Page No.: 8619-8624

DOI: https://dx.doi.org/10.16965/ijar.2023.109

Resin cast in situ: A technique to demonstrate the vasculature of the Nervous system: In Goat Model

Ravindra Kumar B *1, K Thyagaraju 2, Subhadra Devi Velichety 3.

*1 Assistant Professor, Department of Anatomy, Parul Institute of Medical Sciences & Research, Parul University, Vadodara, Gujarat, India. ORCiD: 0000-0003-0569-1472

2 Assistant Professor, Department of Anatomy, Sri Padmavathi Medical College for Women, SVIMS University, Tirupati, Andhra Pradesh, India. ORCiD: 0009-0006-3244-6308

3 Professor and Head, Department of Anatomy, Apollo Institute of Medical Sciences and Research, Chittoor, Andhra Pradesh, India. ORCiD: 0000-0002-3995-8449

Corresponding Author: Dr. Ravindra Kumar B, Assistant Professor, Department of Anatomy, Parul Institute of Medical Sciences & Research, Parul University, Vadodara, Gujarat, India.
E-Mail: dr.ravindrakumar@gmail.com


Background: As Anatomical education advances very rapidly, and at the same time, there is huge demand and scarcity of true biological models, mainly brain tissue.  In this scenario, in 1977, Guther Von Hagens worked on plastics and experimented voraciously on diffusing various plastics into large specimens and ultimately succeeded and coined the term “Plastination”. This technique is very popular in Western countries as the latter greatly minimizes the health hazards due to formalin exposure while dealing with biological tissue. In continuation with the advancement of expensive plastination to cost-effective resin casting, especially studies over the complex structures like the brain and spinal cord.  In This study, we emphasize the cost-effective Resin cast-in-situ method to demonstrate the vasculature of the brain and spinal cord.

Materials and Methods: After obtaining the prior permission, we collected the Five (5) Goat heads from the slaughterhouse. Following the standard dissection procedure over the neck region, identified and canulated the major neck vessels. Through the vessels normal saline water, formalin, and pigmented resin were administered and preserved the goat head using routine preservation technique. After 36 to 48 hours, the routine dissection was scheduled to expose the brain and upper spinal cord segments, and finding were captured and recorded.

Results and Discussion: The specimens show good penetration of dye in the artery and veins, and it’s easy to appreciate and study the vasculature of the brain and upper spinal cord segments, including the Bastons plexus of veins.

Conclusion:  In comparison with regular silicon casting, resin casting is very cost-effective and long-lasting, with good penetration of the resin substance up to the capillary level. Furthermore, similar studies may be conducted using in combination with whole organ plastination using silicon and resin embedding.        

KEYWORDS:  Resin cast in situ, Brain Vasculature, Plastination, Silicon Casting.


[1]. von HAGENS G. Heidelberg plastination folder. Anatomisches Institut I, Universit at Heildel berg 1986.
[2]. von Horst C, von Hagens R, Sora CM, Henry RW. History and development of plastination techniques. Anat Histol Embryol. 2019 Nov;48(6):512-517.
[3]. Soubam P, Mishra S, Suri A, Dhingra R, Mochan S, Lalwani S, Roy T S, Mahapatra AK. Standardization of the technique of silicon injection of human cadaveric heads for opacification of cerebral vasculature in Indian conditions. Neurol India 2018;66:439-43.
[4]. Hagens GV. Impregnation of soft biological specimens with thermos setting resins and elastomers. The Anatomical Record 1979; 194: 247-255.
[5]. Sanan A, Abdel Aziz KM, Janjua RM, van Loveren HR, Keller JT. Colored silicone injection for use in neurosurgical dissections: Anatomic technical note. Neurosurgery 1999;45:1267-74.
[6]. Edirisinghe S, Kotalawala H, De Fonseka D, Dissanayake H, Yasewardene S. Room temperature resin casting technique, a low-cost and effective teaching tool in human anatomy. Sri Lanka Anatomy Journal, 2017;1(1):53-59.
[7]. Fraser Chisholm, Ourania Varsou. Resin-embedded anatomical cross-sections as a teaching adjunct for medical curricula: is this technique an alternative to potting and plastination? J. Anat. 2018;233:98-105.
[8]. Pirici, Ionica, Liliana Cercelaru, Diana Iulia Stanca, Andrei Osman, Lorena Sas, Daniel Pirici, and Ion Mindrila. Simple Universal Whole-Organ Resin-Embedding Protocol for Display of Anatomical Structures. Biomedicines 2023;11(5):1433.

Cite this article: Ravindra Kumar B, K Thyagaraju, Subhadra Devi Velichety. Resin cast in situ: A technique to demonstrate the vasculature of the Nervous system: In Goat Model. Int J Anat Res 2023;11(2):8619-8624. DOI: 10.16965/ijar.2023.109