IJAR.2022.252

Type of Article:  Original Research

Volume 10; Issue 4 (December 2022)

Page No.: 8512-8522

DOI: https://dx.doi.org/10.16965/ijar.2022.252

Effects of Vitamin D On Cisplatin Induced Heart and Lung Toxicity in Albino Rat

Sally Mahmoud Mohamed Hussein Omar 1, Marwa Mohamed Abd El Aziz Ahmed 2, Ola Abd El-Samie Mohamed Khalil *3, and Marwa Mahmoud Mady 4.

1 Lecturer, Department of Anatomy and Embryology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.

2 Lecturer, Pathology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt.

*3 Assistant Lecturer, Department of Anatomy and Embryology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.

4 Lecturer, Department of Anatomy and Embryology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.

ORCiD:

Sally Mahmoud Mohamed Hussein Omar: 0000-0001-6601-4243

Marwa Mohamed Abd El Aziz Ahmed: 0000-0003-4230-7775

Ola Abd El-Samie Mohamed Khalil: 0000-0002-0728-5761

Marwa Mahmoud Mady: 0000-0003-2318-7368

Corresponding author: Ola Abd El-Samie Mohamed Khalil, Assistant Lecturer, Department of Anatomy and Embryology, Faculty of Medicine, Alexandria University, Alexandria, Egypt.
E Mail: Ola.khalil@alexmed.edu.eg

ABSTRACT

Background:  While Cisplatin (CP) is a powerful DNA alkylating agent used to treat many malignancies, its clinical use is linked to a number of negative side effects. It has been proposed that vitamin D can shield biological systems against harm caused by CP. The current study’s objective was to look into how vitamin D protects the rat heart and lung against cisplatin-induced damage.

Material and methods: Thirty adult male Albino rats; 180–220 g body weight were allocated into 3 groups; Group I (n=10) receiving saline, Group II (n=10); rats receiving CP (single dose of 6.5 mg/kg intraperitoneal) and Group III (n=10); receiving CP and 50 ng/kg/day alfacalcidol.

Results: Alterations included a significant increase in malondialdehyde (MDA) level in the CP group compared with the other groups (p value for comparing between control and each other group, statistically significant at p ≤ 0.05). Histopathologically, CP induced severe changes were observed. However, the CP-induced disturbances significantly improved by treatment with Vitamin D.    

Conclusion: According to this study, CP treatment significantly harmed rats’ hearts and lungs; however, treatment with vitamin D significantly lessened these harms.

Keywords: Cisplatin, Immunohistochemical, Vitamin D, Malondialdehyde, Oxidative stress.

REFERENCES

[1]. Addie, Diane D et al. Disinfectant Choices in Veterinary Practices, Shelters and Households: ABCD Guidelines on Safe and Effective Disinfection for Feline Environments. Journal of feline medicine and surgery 2015;17(7): 594–605.
[2]. Chaudhari, L K et al. Antimicrobial Activity of Commercially Available Essential Oils against Streptococcus Mutans.” J Contemp Dent Pract 2012;13(1): 71–74.
[3]. Ismaili, Nabil, Mounia Amzerin, and Aude Flechon. Chemotherapy in Advanced Bladder Cancer: Current Status and Future. Journal of hematology &oncology 2011;4:35. https://pubmed.ncbi.nlm.nih.gov/21906310.
[4]. Dasari, Shaloam, and Paul Bernard Tchounwou. Cisplatin in Cancer Therapy: Molecular Mechanisms of Action. European journal of pharmacology 2014;740: 364–78.
[5]. K.P. Kang, et al., Alpha-lipoic acid attenuates cisplatin-induced acute kidney injury in mice by suppressing renal inflammation, Nephrol. Dial. Transplant. 2009;24(10):3012–3020.
[6]. Y. Chen, P. Jungsuwadee, M. Vore, D. A. Butterfield, and D. K. St Clair. Collateral damage in cancer chemotherapy: oxidative stress in nontargeted tissues. Molecular Interventions 2007;7(3):147–156.
[7]. F. Leo, G. Pelosi, A. Sonzogni, M. Chilosi, G. Bonomo, and L. Spaggiari, “Structural lung damage after chemotherapy: fact or fiction?,” Lung Cancer, 2010;67(3):306–310.
[8]. R. Pratibha, R. Sameer, P. V. Rataboli, D. A. Bhiwgade, and C. Y. Dhume. Enzymatic studies of cisplatin induced oxidative stress in hepatic tissue of rats. European Journal of Pharmacology, 2006;532(3):290–293.
[9]. Ali, B.H. and Al, M.M. Agents ameliorating or augmenting the nephrotoxicity of cisplatin and other platinum compounds: a review of some recent research. Food Chem. Toxicol., 2006;44:1173-1183.
[10]. Yagmurca, M., Bas, O., Mollaoglu, H., Sahin, O., Nacar, A., Karaman, O., and Songur, A. Protective effects of erdosteine on doxorubicin-induced hepatotoxicity in rats. Arch. Med. Res., 2007;38:380-385.
[11]. Walentowicz-Sadlecka M, Sadlecki P, Walentowicz P, Grabiec M. [The role of vitamin D in the carcinogenesis of breast and ovarian cancer]. Ginekol Pol 2013; 84: 305-308.
[12]. Bae S, Singh SS, Yu H, Lee JY, Cho BR, Kang PM. Vitamin D signaling pathway plays an important role in the development of heart failure after myocardial infarction. J Appl Physiol2013;114:979-987.
[13]. Assalin HB, Rafacho BP, dos Santos PP, Ardisson LP, Roscani MG, Chiuso-Minicucci F, et al. Impact of the length of vitamin D deficiency on cardiac remodeling. Circ Heart Fail 2013;6: 809-816.
[14]. M.M. El-Sawalhi, L.A. Ahmed, Exploring the protective role of apocynin, a specific NADPH oxidase inhibitor, in cisplatin-induced cardiotoxicity in rats, Chem. Biol. Interact. 2014;207:58–66.
[15]. Li XW, Feng LX, Zhu XJ, Liu Q, Wang HS, Wu X, et al. Human umbilical cord blood mononuclear cells protect against renal tubulointerstitial fibrosis in cisplatin-treated rats. Biomed Pharmacother. 2020; 121:109662.
[16]. Moneim LMA, Helmy MW, El-Abhar HS. Co-targeting of endothelin-A and vitamin D receptors: a novel strategy to ameliorate cisplatin-induced nephrotoxicity. Pharmacol Rep. 2019;71(5):917-25.
[17]. T. Afsar, S. Razak, A. Almajwal, and M. R. Khan. Acacia hydaspica R. Parker ameliorates cisplatin induced oxidative stress, DNA damage and morphological alterations in rat pulmonary tissue. BMC Complementary and Alternative Medicine 2018;18 (1).article 49.
[18]. A. Kisaoglu, B. Borekci, O. E. Yapca, H. Bilen, and H. Suleyman. Tissue damage and oxidant/antioxidant balance. The Eurasian Journal of Medicine, 2013;45 (1):47–49.
[19]. R. F. Del Maestro. An approach to free radicals in medicine and biology. Acta Physiologica Scandinavica. Supplementum, 1980;492:153–168.
[20]. Antunes LMG, Darin JDC, Bianchi MLP. Protective effects of vitamin C against cisplatin-induced nephrotoxicity and lipid peroxidation in adult rats. Pharmacol Res 2000; 41:405–11.
[21]. Silva CR, Greggi Antunes LM, Bianchi ML. Antioxidant action of bixin against cisplatin-induced chromosome aberrations and lipid peroxidation in rats. Pharmacol Res 2001; 43:561–6.
[22]. Y. Chen, P. Jungsuwadee, M. Vore, D. A. Butterfield, and D. K. St Clair, “Collateral damage in cancer chemotherapy: oxidative stress in nontargeted tissues,” Molecular Interventions, 2007;7(3):147–156.
[23]. R. Pratibha, R. Sameer, P. V. Rataboli, D. A. Bhiwgade, and C. Y. Dhume. Enzymatic studies of cisplatin induced oxidative stress in hepatic tissue of rats. European Journal of Pharmacology 2006;532(3):290–293.
[24]. F. Leo, G. Pelosi, A. Sonzogni, M. Chilosi, G. Bonomo, and L. Spaggiari. Structural lung damage after chemotherapy: fact or fiction?, Lung Cancer, 2010;67(3):306–310.
[25]. J. A. Cooper Jr., D. A. White, and R. A. Matthay. Drug-induced pulmonary disease. Part 1: cytotoxic drugs. The American Review of Respiratory Disease, 1986;133 (2):321–340.
[26]. İ. Topal, A. Özbek Bilgin, F. Keskin Çimen et al., The effect of rutin on cisplatin-induced oxidative cardiac damage in rats. The Anatolian Journal of Cardiology, 2018;20:136–142.
[27]. A.A. Al-Majed, M.M. Sayed-Ahmed, A.A. Al-Yahya, A.M. Aleisa, S.S. Al-Rejaie, O.A. Al-Shabanah, Propionyl-L-carnitine prevents the progression of cisplatin-induced cardiomyopathy in a carnitine-depleted rat model, Pharmacol. Res. 2006;53(3):278–286.
[28]. S.H. Ismail, A.M. Attyah, Protective effect of ginger extract against cisplatin-induced hepatotoxicity and cardiotoxicity in rats, Iraqi J. Pharm. Sci. 2012;21(1):27–33.
[29]. M. FLORESCU, M. CINTEZA, D. VINEREANU, Chemotherapy-induced cardiotoxicity, Maedica 2013;8(1):59.
[30]. Z.V. Varga, P. Ferdinandy, L. Liaudet, P. Pacher, Drug-induced mitochondrial dysfunction and cardiotoxicity, Am. J. Physiol. Heart Circ. Physiol. 2015;309(9):H1453–H1467.
[31]. M.M. El-Sawalhi, L.A. Ahmed, Exploring the protective role of apocynin, a specific
NADPH oxidase inhibitor, in cisplatin-induced cardiotoxicity in rats, Chem. Biol. Interact. 2014;207:58–66.
[32]. P. Zhang, L.-h. Yi, G.-y. Meng, H.-y. Zhang, H.-h. Sun, L.-q. Cui, Apelin-13 attenuates cisplatin-induced cardiotoxicity through inhibition of ROS-mediated DNA damage and regulation of MAPKs and AKT pathways, Free Radic. Res. 2017;51 (5):449–459.
[33]. M. Khan, J.C. Shobha, I.K. Mohan, M.U.R. Naidu, C. Sundaram, S. Singh, P. Kuppusamy, V.K. Kutala, Protective effect of Spirulina against doxorubicin‐induced cardiotoxicity, Phytother. Res. 2005;19(12):1030–1037.
[34]. A.V. Swamy, U. Wangikar, B. Koti, A. Thippeswamy, P. Ronad, D. Manjula, Cardioprotective effect of ascorbic acid on doxorubicin-induced myocardial toxicity in rats, Indian J. Pharmacol. 2011;43(5):507.
[35]. N. Santos, C. Catao, N. Martins, C. Curti, M. Bianchi, A. Santos, Cisplatin-induced nephrotoxicity is associated with oxidative stress, redox state unbalance, impairment of energetic metabolism and apoptosis in rat kidney mitochondria, Arch. Toxicol. 2007;81 (7):495–504.
[36]. K.A. Conklin, G.L. Nicolson, Molecular replacement in cancer therapy: reversing cancer metabolic and mitochondrial dysfunction, fatigue and the adverse effects of therapy, Curr. Cancer Ther. Rev.2008;4:66–76.
[37]. J.-S. Kim, L. He, J.J. Lemasters, Mitochondrial permeability transition: a common pathway to necrosis and apoptosis, Biochem. Biophys. Res. Commun. 2003;304(3):463–470.
[38]. Wimalawansa SJ. Vitamin D deficiency: Effects on oxidative stress, epigenetics, gene regulation, and aging. Biology (Basel). 2019;8:30.
[39]. Norman AW, Nemere I, Zhou LX, et al. 1,25(OH)2-vitamin D3, a steroid hormone that produces biologic effects via both genomic and nongenomic pathways. J Steroid Biochem Mol Biol. 1992;41:231-40.
[40]. Wang H, Chen W, Li D, et al. Vitamin D and chronic diseases. Aging Dis. 2017;8:346-53.
[41]. Wiseman H. Vitamin D is a membrane antioxidant Ability to inhibit iron-dependent lipid peroxidation in liposomes compared to cholesterol, ergosterol and tamoxifen and relevance to anticancer action. FEBS Lett. 1993;326:285-8.
[42]. Câmara AB, Brandão IA. The relationship between vitamin D deficiency and oxidative stress can be independent of age and gender. Int J Vitam Nutr Res. 2019:1-16.
[43]. BaSalamah MA, Abdelghany AH, El-Boshy M, et al. Vitamin D alleviates lead induced renal and testicular injuries by immunomodulatory and antioxidant mechanisms in rats. Sci Rep. 2018;8:1-13.
[44]. Liu Y, He Y, Wang Q, et al. Vitamin D 3 supplementation improves testicular function in diabetic rats through peroxisome proliferator-activated receptor-γ/transforming growth factor-beta 1/nuclear factor-kappa B. J Diabetes Investig. 2019;10:261-71.
[45]. Jørgensen A, Jensen MB, Nielsen JE, et al. Influence of vitamin D on cisplatin sensitivity in testicular germ cell cancer-derived cell lines and in a NTera2 xenograft model. J Steroid Biochem Mol Biol. 2013;136:238-46.

Cite this article: Sally Mahmoud Mohamed Hussein Omar, Marwa Mohamed Abd El Aziz Ahmed, Ola Abd El-Samie Mohamed Khalil, and Marwa Mahmoud Mady. Effects of Vitamin D On Cisplatin Induced Heart and Lung Toxicity in Albino Rat. Int J Anat Res 2022;10(4):8512-8522. DOI: 10.16965/ijar.2022.252