Type of Article:  Original Research

Volume 8; Issue 1.1 (January 2020)

Page No.: 7256-7270

DOI: https://dx.doi.org/10.16965/ijar.2019.355


Shimaa Anter Fareed *¹´², Eman Mohamed Kamel ¹, Nassar Ayoub ²,³.

*¹ Human Anatomy and Embryology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.

² Anatomy Department, Al-Rayan Colleges for medical Studies, AL Madinah, Saudi Arabia.

³ Anatomy Department, Faculty of Medicine, Suhag University.

Corresponding Author: Dr. Shimaa Anter Fareed, Human Anatomy and Embryology Department, Faculty of Medicine, Suez Canal University, Ismailia, Egypt. E-Mail: shimaa_ant@hotmail.com


Nitrate (NO3) and nitrite (NO2) are naturally-occurring oxidation products of nitrogen which widely used in the food industry. The goal of this study was to illustrate the histopathological effects of sub-lethal dose of sodium nitrite on jejunal mucosa and the possible protective effect of vitamin A. Thirty adult male Albino rats were divided into 3 groups as follows: Group I (control) received distilled water, Group II (Nitrite-treated) which received intra-gastric daily dose of 50 mg/kg NaNO2  and Group III (Nitrite & vit-A treated group) which received intra-gastric daily dose of 50 mg/kg NaNO2 +10,000 I.U/ rat vitamin A. Animals were sacrificed 8 weeks after treatment. Blood samples were collected and examined. Specimens from jejunal mucosa of rats were collected for the optical and electron microscope study. The blood samples showed significant reduction in all blood cells counting and albumin level with elevated methemoglobin level in nitrite treated group. Light microscopic examination revealed that; Sodium nitrite cause jejunal mucosa damage represented by abnormal shape and length of jejunal mucosa villi, necrosis with marked loss of covering epithelium and excessive cellular infiltration in its core of lamina. PAS stained sections exhibited weak or negative PAS reaction of goblet cells of brush border of villi and crypts. The entrocytes and goblet cells showed ultrastructural changes. Vitamin A administration resulted in marked regression of the previously mentioned jejunal mucosal effects. Results of the current study revealed that diatry supplemtation of nitrite cause multible jejunal mucosal injury but the co-administration of Vitamin A greatly reduce the toxic effects of nitrite supplementation on jejunal mucosa.

Key word: Sodium nitrite, Vitamin-A,  Jejunal mucosa, Histopathology, Ultra-structure.


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Cite this article: Shimaa Anter Fareed, Eman Mohamed Kamel, Nassar Ayoub. THE EFFECTS OF DIETARY SODOIUM NITRITE SUPPLEMENTATION ON JEJUNAL MUCOSA AND POSSIBLE PROTECTIVE EFFECT OF VITAMIN A IN ALBINO RATS. Int J Anat Res 2020;8(1.1):7256-7270. DOI: 10.16965/ijar.2019.355