https://www.ijmhr.org/ijar.htm editor_anatomy@ijmhr.org
ISSN:       2321-4281 (E) 2321-8967 (P)

IJAR.2019.338

DOWNLOAD PDF
Table of Contents

Type of Article:  Original Research

Volume 7; Issue 4.3 (December 2019)

Page No.: 7187-7192

DOI: https://dx.doi.org/10.16965/ijar.2019.338

HISTOMORPHOMETRIC CHANGES OF GONADS IN MALE GUINEA PIGS AFTER THYMECTOMY

P. Murali 1, J. Radhika *2.

*1 Research scholar, Department of Anatomy, SRM Medical College Hospital and Research Centre, Kattankulathur,Tamilnadu,India.

2 Professor, SRM Medical College Hospital and Research Centre, Kattankulathur, Tamilnadu, India.

Address for Correspondence: Dr. J. Radhika, Professor, SRM Medical College Hospital and Research Centre, Kattankulathur, Tamilnadu, India. E-Mail: pmurali.pt@gmail.com

ABSTRACT

Introduction: The presence of the thymus gland during early life is essential for the normal development of immune system and as well as proper maturation of the hypothalamic – pituitary- gonadal axis.  This study was to investigate the influence of thymus gland on male reproductive function.

Aim: The aim of this study to investigate the effect of thymectomy on Morphometric changes of gonads in neonatal, prepubertal and pubertal thymectamized male guinea pigs.  

Material and Methods: In this study, a total of thirty male guinea pigs, thymectamized (n-6) and sham-operated (n-4) were studied in each group. The comparison of diameter of seminiferous tubules, luminal diameter and epithelial thickness in the thymectamized and sham operated male guinea pigs.

Results: No Significant changes in the morphometry of seminiferous tubules of testis in thymectamized male guinea pigs compared to the sham-operated guinea pigs in all age groups. These results suggest that thymectomy had no detrimental effects on reproductive system in males at any intervals.

Key words: Thymectomy, Seminiferous diameter, Luminal diameter and Epithelial thickness.

REFERENCES

  1. Safieh B, Kendall MD, Norman JC, Metreau E, Dardenne M, Bach J-F & Pleau JM A new radioimmunoassay for the thymic peptide thymulin and its application for measuring thymulinin blood samples. Journal of Immunological Methods 1990; 127: 255-262.
  2. Hinojosa L, Chavira R, Dominguez R & Rosa P Effect of thymulin on spontaneous puberty and Gonadotrophins – induced ovulation in prepubertal normal and hypothymic Mice. Journal of Endocrinology 1999; 163: 255-260.
  3. Celok Lek, Interaction involving the thymus and the hypothalamus – pituitary axis, immunomodulation by hormones. Srp Arh 2004;132:187-93.
  4. Nishizuka, Y & Sakakura, T. Thymus and reproduction: sex‐linked dysgenesia of the gonad after neonatal thymectomy in mice. Obstet Gynecol Surv 1970;25: 353– 355.
  5. Nishizuka, Y & Sakakura, T. Ovarian dysgenesis induced by neonatal thymectomy in the mouse. Endocrinology 1971;89:886– 893.
  6. Rebar, RW, Morandini, I, Erickson, GF & Petze, JEThe hormonal basis of reproductive defects in athymic mice: diminished gonadotropin concentrations in prepubertal females. Endocrinology 1981;108:120– 126.
  7. Farookhi, R, Wesolowski, E, Trasler, J & Robaire, B. Modulation by neonatal thymectomy of the reproductive axis in male and female rats during development. Biol Reprod 1988;38:91– 99.
  8. Andersson, M. Relationships between GnRH‐induced testosterone maxima, sperm motility and fertility in Ayrshire bulls. Anim Reprod Sci 1992;27;107– 111.
  9. Bloom, DF, Bloch, GJ & Gorski, RA. Effects of thymectomy on reproductive function and behavior. Physiol Behav 1992;52:291– 298.
  10. Jin, Z, Yong‐shang, Z, Pomer, S, Staehler, G & Da‐hong, R. The influence of thymectomy on germ‐cells of the testis in mice. J Tongji Med Univ 1995;15:112– 116.
  11. Su, S, Fang, F, Liu, Y, Li, Y, Ren, C, Zhang, Y & Zhang, X. The compensatory expression of reproductive hormone receptors in the thymus of the male rat following active immunization against GnRH. Gen Comp Endocrinol 2013;185: 57– 66.
  12. Sharma, R, Kantwa, SM, Jaitawat, A, Rani, D & Jain, N. Relationship between testis and thymus during postnatal development in Swiss mice. Univ J Environ Res Tech 2014;4:208– 214.
  13. A. Recherches experimentales sur un rapport probable entre la function du thymus et cells des testicules, Arch.ital.Biol. Torino. 1898;30:71-77.
  14. Dougherty SM, Mazhawidza W, Bohn AR, Robinson KA, Mattingly KA, Blankenship KA, Huff MO, McGregor WG, Klinge CM Gender difference in the activity but not expression of estrogen receptors alpha and beta in human lung adenocarcinoma cells. Endocr Relat Cancer. 2006;13(1):113-34.
  15. Lintern-Moore. Effect of athymia on the initiation of follicular growth in the rat ovary. Biol Reprod 1977;17:155-61.
  16. Hattori M,Brandon MR,Thymus and the endocrine systemOvarian dygenesis in neonatally thymectomized rats.J Endocrinol. 1979;83(1) : 101-11.
  17. Deschaux, P, Massengo, B & Fontanges, R. Endocrine interaction of the thymus with the hypohysis, adrenals and testes: effects of two thymic extracts. 1979;Thymus 1: 95– 108.
  18. Michael SD, Taguchi O, Nishizuka Y. Effects of neonatal thymectomy on ovarian development and plasma LH, FSH, GH and PRL in the mouse. Reprod. 1980;22:343–350.
  19. Kincl, F.A., Folch PI, A., Maqueo, M., Lasso, L.H., Dorfman, and P.J. Inhibition of sexual development in male and female rats treated with various steroids at the age of five days, Acta endocr. 1965;49: 193-206.
  20. Robert W Rebar,I C Morandini,J E Petze,G F Erickson. Hormonal basis of reproductive defects in athymic mice: Reduced gonadotropins and testosterone in males. Biology of Reproduction 1983:27(5):1267-76.
  21. Patson D.N. The Thymus and Sexual organs. Their Relationship to the growth of the animal. J. Physiol. 1911;42: 267.
  22. Adams DB.The effect of thymectomy in guinea pigs on the lymphocyte content of central lymph.Aust J ExpBiol Med Sci. 1977;55(1): 49-57.
  23. Temidayo S O, Stefan S d. Diabetes mellitus and male infertility. Asian Pac J Reprod 2018;7:6-14.
  24. CHEMES,H.E.,DYM,M.,FAWCETr,D.W.,JAVADPOUR,N.&SHERINS,R.Patho-physiological observations of Sertolicellsinpatients With germinal aplasia or severe germ cell depletion.Ultra-structural findings and hormone levels.Andrologia 1977;17:108-123.
  25. Plagge, J.C. (1941) Thymus gland in relation to sex hormones and reproductive procecesses in albino rats J.morphol.1941;68:519.
  26. Yasuaki Nishizuka, Teruyo Sakakura.Thymus and Reproduction: Sex-Linked Dysgenesia of the Gonad after Neonatal Thymectomy in Mice. 1969;166 (3906): 753-755.
  27. Robert W. Rebar C. Morandini John E. Petze Gregory F. Erickson. Hormonal Basis of Reproductive Defects in Athymic Mice: Reduced Gonadotropins and Testosterone in Males. Biology of Reproduction, 1982;27(5) : 1267–1276.
  28. Vos JO. Berkvens JM. Kruijt BC. The athymic nude rat I. Morphology of lymphoid and endocrine organs. Clipi Immuno and Imniunopathol 1980;15:213-228.
  29. Ommati MM, Heidari R, Zamiri MJ, Shojaee S, Akhlaghi A, Sabouri S. Association of open field behavior with blood and semen characteristics in roosters: an alternative animal model. Rev Int Androl.2018;16(2):50-58.
  30. Bartke A, Dalteiro. Effects of prolactin on the sensitivity of the testis to LH. Biol Reprod 1976;15:90-93
  31. Shire, J.G.M. & Pantelouris, E.M. Comparison of endocrine function in normal and genetically athymic mice. Comparative biochemistry and physiology. A, Comparative physiology. 1974;47: 93-100.
  32. Zipper J., Tatum H.J., Medel M., Pastene L. and Rivera M. Presented at the 15th Nobel Symposium on Control of Human Fertility. 1970;May 27–29.
  33. Dym M, Clermont Y. Role of spermatogonia in the repair of the seminiferous epithelium following X-irradiation of the rat testis.  J. Anat. 1970;128:265–282.
  34. Kincl F.A., Rudel H.W. Possible Mechanism of the Anti-Fertility Effect of Copper Current Problems in Fertility1971;pp 187-190.
  35. Potapov, A., Merrill, E., Pybus, M., & Lewis, M. A.. Chronic Wasting Disease: Transmission Mechanisms and the Possibility of Harvest Management. PloS one, 2016;11(3).
  36. Guaragna, Regina & B. S. Rosa, Neila & G. S. Moraes, Galba & L. S. Perry, Marcos & A. Bernard, Elena. Effect of protein malnutrition on glycoprotein synthesis by testes of 20‐day‐old rats. International journal of andrology. 1987;9: 467-76.
  37. Smith NM, Byard RW, Bourne AJ Testicular regression syndrome–a pathological study of 77 cases. Histopathology 1991;19:269–272.

Cite this article: P. Murali, J. Radhika. HISTOMORPHOMETRIC CHANGES OF GONADS IN MALE GUINEA PIGS AFTER THYMECTOMY. Int J Anat Res 2019;7(4.3):7187-7192. DOI: 10.16965/ijar.2019.338