IJAR.2019.206

Type of Article:  Original Research

Volume 7; Issue 3.1 (July 2019)

Page No.: 6722-6726

DOI: https://dx.doi.org/10.16965/ijar.2019.206

COMPARATIVE EVALUATION OF THE HEPATOPROTECTIVE EFFECT OF COSTUS PICTUS D DON METHANOLIC LEAF EXTRACT AND SILYMARIN ON PARACETAMOL INDUCED LIVER DAMAGE IN ALBINO WISTAR RATS

Anitha Nancy 1, Jeneth Berlin Raj *2, Manimekalai K 3.

1 Assistant Professor, Department of Anatomy, Mahatma Gandhi Medical College & Research Institute, Sri Balaji Vidyapeeth’ (Deemed to be university), Puducherry, India.

2 Professor, Department of Physiology, Mahatma Gandhi Medical College & Research Institute, Sri Balaji Vidyapeeth’ (Deemed to be university),  Puducherry, India.

3 Professor, Department of Pharmacology, Mahatma Gandhi Medical College & Research Institute, Sri Balaji Vidyapeeth’ (Deemed to be university), Puducherry, India.

Corresponding Author: Dr. Jeneth Berlin Raj, Professor, Department of Physiology, Mahatma Gandhi Medical College & Research Institute, Sri Balaji Vidyapeeth’ (Deemed to be university), Puducherry, India. E-Mail: ancyjean2010@gmail.com

ABSTRACT:

Background: NSAIDs are the common group of drugs used in self-medication, and this is true for especially Paracetamol (acetaminophen).Although considered safe at therapeutic doses, in overdose, paracetamol causes centrilobular hepatic necrosis which can be fatal. As no data is available on the hepatoprotective effect of Costus pictus D Don, we have made an attempt to investigate the protective effect of Costus pictus D Don leaf extract on paracetamol induced liver damage in rats. The aim of the study is to compare the hepatoprotective effect of methanolic leaf extract of Costus pictus D Don and silymarin on liver damage induced by paracetamol in Wistar rats.

Materials and Methods: 30 Healthy male adult Wistar rats (16 weeks old) weighing > 250g were used for the study. The animals will be maintained in a standard cage under controlled temperature (25+2 °C) and light (12:12 light-dark cycle) in MGMC & RI central animal house. The animals were fed with standard rat pellet and hygienic water ad libitum.30 adult Wistar rats will be randomized into 5 groups with 6 rats each as (Normal control -0.5% carboxymethylcellulose (7 days), Toxic control- 0.5% (7 days)+paracetamol 2g/kg(5th day), Test group I-200 mg/kg methanolic leaf extract+paracetamol 2g/kg(5th day) , Test group II-100 mg/kg methanolic leaf extract+paracetamol 2g/kg(5th day)  & Standard group – silymarin 25mg/kg (7 days) + Paracetamol 2 g/kg (5th day) . The animals were sacrificed on 8th day using sodium pentobarbitone 150mg/kg i.p. serum was sent for biochemical analysis for liver function test. Liver was harvested and a portion was taken for histological examination.

Results: In our study methanolic leaf extract of Costus pictus D Don showed beneficial effect on paracetamol induced liver toxicity which was evident by the significant improvement in liver function test consisting of AST, ALT and ALP in a dose dependent manner which is in consistent with the histological findings.

Conclusions: The study has proved the methanolic leaf extract of Costus pictus D Don posses a significant hepatoprotective activity which was comparable to the standard drug silymarin.

Key words: Costus pictus D Don, Silymarin, Hepatoprotection.

REFERENCES

  1. Bruden P (1988). World drug situation. Geneva: WHO.
  2. World Health Organization (1998) The Role of pharmacist in Heath Care System; Available from: http://www.apps.who.int/medicinedocs/en/d/ Jwhozip32e [last accessed on 2016 Jan19].
  3. Abay SM, Amelo W. Assessment of self-medication practices among medical, pharmacy and health science students in Gondar University, Ethiopia. J Young Pharm.2010; 2 (3): 306-310.
  4. Prescott LF . Hepatotoxicity of mild analgesics. Br J Clin Pharmacol.1980;10(2): 373 S- 379S.
  5. Laura PJ, Philip RM, Jack AH. Acetaminophen-induced hepatotoxicity. ASPET journals.2003; 31 (12): 1499 – 1506.
  6. F.El-kott, M.M. Bin-Meferij. Use of Aractiumlappa against acetaminophen-induced hepatotoxicity in rats. Current therapeutic research.2015; 77: 73-78.
  7. Mishra S, Aeri V, Katare DP. Hepatoprotective medication for liver injury. WJPPS.2014; 3(5): 891-932.
  8. Suganya S, Narmatha R, Gopalakrishnan VK, Devaki K. Hypoglycaemic effect of Costus pictus D. Don on alloxan induced type 2 diabetes mellitus in albino rats. Asian Pacific Journal of Tropical Disease.2012: 117-123.
  9. Nandumane VK, Rajashekar S, Narayana P, Adinarayana S, Vijayan S, Prakash S, Sharma A . Evaluation of the anti-cancer potential of costuspictus on fibrosarcoma (HT-1080) cell line. J Natural Pharmaceutical.2011; 2(2): 72-76.
  10. Majumdar M, Parihar PS. Antibacterial and antiglycation potential of Costuspictus from southern region, India. Asian J Plant Sci Res.2012; 2(2): 95-101.
  11. Raj JB, Kalaivani R. Comparative in-vitro evaluation of anthelmintic property of leaves and rhizome of Costus pictus D. Don against albendazole. Natl J Physiol Pharm Pharmacol 2016;6(5):438-441.
  12. Raj JB, Kalaivani R. In-vitro evaluation of antimicrobial activity of Costus pictus D. Don aqueous leaf extract. Natl J Physiol Pharm Pharmacol 2018;8(8):1107-1109.
  13. Almajwal AM, Elsadek MF. Lipid-lowering and hepatoprotective effects of Vitis vinifera dried seeds on paracetamol-induced hepatotoxicity in rats. Nutrition research and 2015;9(1):37-42.
  14. Reuben A. Hy’s law. Hepatology.2004;39(2):574-8.
  15. Sheehan M, Haythorn P. Liver function tests and their interpretation. Indian J  2007;74(7):663-71.
  16. Kazemifar AM, Hajaghamohammadi AA, Samimi r, Alavi Z, Abbasi E, Asl MN. Hepatoprotective property of oral silymarin is comparable to n-acetyl cysteine in acetaminophen poisoning.Gastroenterol Res.2012;5(5):190-4.
  17. Muriel P, Garciapina T, Perez-Alvarez V, Mourelle M. Silymarin protects against paracetamol-induced lipid peroxidation and liver damage. J Appl Toxicol.1992;12(6):439-42.
  18. Zhang X, Ouyang J, Thung SN. Histopathological manifestations of drug induced hepatotoxicity. Clin Liver Dis. 2013 Nov;17(4):547-64.
  19. Girish C, Koner BC, Jayanthi S, Ramachandra RK, Rajesh B, Pradhan SC. Hepatoprotective activity of picroliv,curcumin and ellagic acid compared to silymarin on paracetamol induced toxicity in mice. Fundam Clin Pharmacol.2009;23(6):735-45.

Cite this article: Anitha Nancy, Jeneth Berlin Raj, Manimekalai K. COMPARATIVE EVALUATION OF THE HEPATOPROTECTIVE EFFECT OF COSTUS PICTUS D DON METHANOLIC LEAF EXTRACT AND SILYMARIN ON PARACETAMOL INDUCED LIVER DAMAGE IN ALBINO WISTAR RATS. Int J Anat Res 2019;7(3.1):6722-6726. DOI: 10.16965/ijar.2019.206