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IJAR.2019.174

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Type of Article:  Original Research

Volume 7; Issue 2.2 (May 2019)

Page No.: 6581-6589

DOI: https://dx.doi.org/10.16965/ijar.2019.174

ANATOMICAL AND HISTOPATHOLOGICAL EFFECTS OF ZINC OXIDE NANOPARTICLES ON LUNGS IN ADULT MALE ALBINO RATS

Nehal Mohamed Nabil *1, Samar El Achy 2.

*1 Anatomy and Embryology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt.

2 Pathology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt.

Correspondence Author: Nehal Mohamed Nabil, Anatomy and Embryology department, Faculty of Medicine, Alexandria University, Alexandria, Egypt.  E-Mail: nehalnabil895@gmail.com

ABSTRACT:

Background: Zinc oxide nanoparticles (ZnO-NPs) are frequently used in many fields, including food industry for their antimicrobial activity. Acute exposure to high doses of such particles was found to be toxic to many organs. However, the lung toxicity resulting from chronic exposure to oral doses of ZnO-NPs was not adequately assessed before.

Aim of the work: to detect the anatomical and histopathological effects of chronic exposure to ingested ZnO-NPs on the lung of normal adult male albino rat.

Material and methods: It was carried out on 30 adult male Swiss albino rats with an average weight of 150-200 gm. They were divided into two groups: Group I: 10 rats serving as control group; Group II: 20 rats serving as experimental groups, divided into 2 subgroups (a&b) receiving oral ingestion by orogastric tube of a single daily dose (125mg/ kg) of average 20 nm sized ZnO-NPs for different durations: Group IIa (n=10):  for 120 days; Group IIb (n=10): for 180 days. Histopathology and immunohistochemistry of the lungs in the three groups was performed to detect the possible effect of such exposure.

Results: Oral administration of ZnO-NPs induced lung damage manifested by congested blood vessels, interstitial inflammation, infiltration with macrophages& lymphocytes, supurative granuloma, thickened interalveolar septa. These changes were more evident with longer exposure for 180 days (P≤0.5). This substantial damage to the lungs is caused by oxidative stress and chronic inflammation.

Conclusion: Caution should be considered when using these particles in food packaging and food additives, and for those who are in close contact with these particles especially in factories.

Key words: ZnO-NPs, Oral ingestion, Immunohistochemistry, lungs.

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Cite this article: Nehal Mohamed Nabil, Samar El Achy. ANATOMICAL AND HISTOPATHOLOGICAL EFFECTS OF ZINC OXIDE NANOPARTICLES ON LUNGS IN ADULT MALE ALBINO RATS. Int J Anat Res 2019;7(2.2):6581-6589. DOI: 10.16965/ijar.2019.174