Type of Article:  Original

Volume 7; Issue 2.1 (April 2019)

Page No.: 6424-6431

DOI: https://dx.doi.org/10.16965/ijar.2019.133


Kebe, E. Obeten *1, Ettah, E. Nkanu 2, Gabriel U. Udo-Affah 3.

*1 Department of Anatomy and Forensic Science, Cross River University of Technology, Okuku, Nigeria.

   2 Department of Physiology, Cross River University of Technolog, Okuku, Nigeria.

   3 Department of Human Anatomy, University of Calabar, Calabar.

Corresponding author: Kebe E. Obeten, Department of Anatomy and Forensic Science, Cross River University of Technology, Okuku, Nigeria. Phone: +2348035505856 E-Mail: fredobeten@yahoo.com


This study was carried out to evaluate the effects of ethanolic leaf extract of Sida acuta on the histology of the testis and reproductive hormones of male adult Wistar rats. Twenty five rats weighing between 180 – 220 g were assigned to five groups (control, olive oil control, low dose, medium dose and high dose) with five animals each. Normal control fed only on rat chow, olive oil control group received 0.5ml of olive oil. The experimental groups, low dose, medium dose and high dose received 500mg/kgBW, 1000mg/kgBW 1500mg/kgBW of the extract orally for 60days respectively. The animals were sacrificed at end of sixty days using chloroform anesthesia. Blood was collected from the left ventricle of the heart through cardiac puncture for hormonal assay. The testes were dissected, sectioned for hematoxylin and eosin (H&E). Hormonal studies showed dose dependent significant (p<0.05) increase in Luteinizing Hormone (LH) across all the treatment groups when compared to the normal and olive oil control groups. Follicle Stimulating Hormone (FSH) was reduced in the low and medium dose animals that received 500mg/kgBw and 1000mg/kgBw of the extract respectively when compared to the control groups, while the high dose group that received 1500mg/kgBw of the extract showed increased FSH level. Testosterone level remained insignificant among all experimental groups when compared to the normal and olive oil control groups. Histological observations of the testes showed dose dependent distortion of the normal cytoarchitecture of the organs, as the high dose (1500mg/kgBw) treated group revealed prominent distortion.  Hence the study suggests that consumption of Sida acuta at high dose may cause adverse effect on reproductive parameters and organs.

Key words: Sida acuta, Luteinizing Hormone, Follicle Stimulating Hormone, Testosterone.


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Cite this article: Kebe, E. Obeten, Ettah, E. Nkanu, Gabriel U. Udo-Affah. HISTO-HORMONAL STUDIES OF THE EFFECT OF Sida acuta ON THE TESTIS OF ADULT WISTAR RATS. Int J Anat Res 2019;7(2.1):6424-6431. DOI: 10.16965/ijar.2019.133