Type of Article:  Original

Volume 7; Issue 1.3 (March 2019)

Page No.: 6254-6260

DOI: https://dx.doi.org/10.16965/ijar.2018.438


N Mugunthan *1, J Anbalagan 2, S Meenachi 3, S Rajanarayanan 4.

*1 Professor, Department of Anatomy, Sree Mookambika Institute of Medical Sciences, Kulasekharam, India.

2 Professor, Department of Anatomy, Mahatma Gandhi Medical College & Research Institute, Puducherry, India.

3 Deputy Director of Health Services, Paramakudi HUD, Tamil Nadu.

4 Professor & H.O.D, Department of Bio-technology, St.Michael College of Engineering &  Technology, Kalayarkoil, India.

Correspondence: Dr.N.Mugunthan. MBBS., M.S., D.N.B., PhD., Professor, Department of Anatomy, Sree Mookambika Institute of Medical Sciences, Padanilam, Kulasekharam, Kanyakumari District, Tamil Nadu, India, Pincode -629161. Mobile: +919443118932

EMail: dr.mugunth111@gmail.com


Background: Rapid developments in the telephone technology made the communication faster and easier. The duration of the use of mobile phones also increased beyond the limitation, and has raised public concerns about their safety. This study is designed to investigate the long term exposure of 1900 MHz to 2200 MHz radiations effect emitted from the third generation (3G) mobile phone in mice liver at histological and biochemical level.

Materials and Methods: With approval from institutional animal ethics committee, 36 mice were exposed to 3G mobile phone radiation, 48 minutes per day for a period of 30 -180 days. The control group (36 mice) was kept under similar conditions without 3G exposure. The animals were sacrificed and the liver was removed from first month to six months period. 1ml of blood was collected for SGOT and SGPT analysis. Liver was processed for antioxidant enzyme activity – SOD & GPx, and for routine histological study. The serial sections were analysed under microscope for structural changes. Two groups’ data were compared and analysed statistically. Results: In comparision to control group the liver of 3G exposed groups showed the following changes: parenchyma and the periportal regions were infiltrated with inflammatory cells. Focal haemorrhages in the liver parenchyma dilated and congested hepatic sinusoids. Swollen hepatocytes with pyknotic nuclei and vacuolations in the cytoplasm were the significant changes. Biochemical parameters did not show any variable difference.

Conclusion: The long term exposure of ultra-high frequency radiation emitted from a 3G mobile phone could cause microscopic structural changes in the liver.

Key words: 3G mobile phone, liver, radiation


  1. International Commission on Non-Ionizing Radiation Protection. Review of the scientific evidence on dosimetry, biological effects, epidemiological observations and health consequences concerning exposure to high frequency electromagnetic fields (100 KHz to 300 GHz). Germany. ICNIRP 16/2009.
  2. Guidelines for Limiting Exposure to Time-varying Electric, Magnetic and Electromagnetic Fields (up to 300 GHz). Health Physics. 1998; 74( 4) : 494-522.
  3. D’Andrea JA, Emmerson RY, Bailey CM, Olsen RG, Gandhi OP. Microwave radiation absorption in the rat: Frequency-dependent SAR distribution in body and tail. Bioelectromagnetics. 1985; 6(2):199-206.
  4. Lai H, Singh NP. Single and double strand DNA breaks in rat brain cells after acute exposure to radiofrequency electromagnetic radiation. Int J Radiat Biol.1996; 69(4):513-21.
  5. Osman Fikret Sonmez, Ersan Odaci, Orhan Bas and Suleyman Kaplan. Purkinje cell number decreases in the adult female rat cerebellum following exposure to 900 MHz electromagnetic field. Brain Research. 2010; 1356: 95-101.
  6. Ashok Agarwal, Nisarg Desai, KartikeyaMakker, Alex Varghese, Rand Mouradi, Edmund Sabanegh, Rakesh Sharma. Effects of radiofrequency electromagnetic waves (RF-EMW) from cellular phones on human ejaculated semen: an in vitro pilot study. Fertil Steril.2008;1318-1325.
  7. Meo SA, Al-DreesAM, Husain S, Khan MM, Imran MB. Effects of mobile phone radiation on serum testosterone in Wistar albino rats. Saudi Med J.2010; 30(8):869-73.
  8. Ntzouni MP, Stamatakis A, Stylianopoulou F, Margaritis LH. Short- term memory in mice is affected by mobile phone radiation. Pathophysiology.2010: 25.
  9. Christoph Augner, Gerhard W. Hacker. Are people living next to mobile phone base stations more strained? Relationship of health concerns, self-estimated distance to base station, and psychological parameters. Indian Journal of Occupational and Environmental Medicine.2009; 13 (3):141-145.
  10. Al-Glaib B, Al-Dardfi M, Al-Tuhami A, Elgenaidi A and Dkhil M. A technical report on the effect of electromagnetic radiation from a mobile phone on mice organs. Libyan J Med.2007; AOP: 080107:8-9.
  11. Laila K. Hanafy, Sawsan H, Karam, AnisaSaleh. The adverse effects of mobile phone radiation on some visceral organs. Research Journal of Medicine and Medical Sciences. 2010; 5(1):95-99.
  12. Latifa Ishaq Khayyat. The histopathological effects of an electromagnetic field on the kidney and testis of mice. EurAsian Journal of BioSciences.2011; 5:103-109.
  13. N Hanafi, F.Eid, A.El-Dahshan.Radiation emitted from mobile phone induces amyloidosis features in some tissues of infant mice. The Egyptian Journal of Hospital Medicine.2012; 47:132-144.
  14. Ingole IV, Ghosh SK. Cell phone radiation and developing tissues in chick embryo: A light microscopic study of kidneys. J. Anat. Soc. India.2006; 55(2):19-23.
  15. Ingole IV, Ghosh SK.Exposure of radiofrequency radiation emitted by cell phone and mortality in chick embryo (gallus domesticus). Biomed Res. 2006; 17(3):205-210.
  16. Shahram Gharachorlou, Amirali Ebrahimneghazd, Hamid Mirzaie. Histopathological study on electromagnetic low frequency (50Hz) effects on induction of apoptosis in liver of rat. Journal of Current Research in Science.  2013; 1( 6) : 620-624.
  17. Belyaev IY, Koch CB, Terenius O, Roxstrom Lindquist K, Malmgren LO, H Sommer W, Salford LG, Persson BR. Exposure of rat brain to 915 MHz GSM microwaves induces changes in gene expression but not double stranded DNA breaks or effects on chromatin conformation. Bioelectromagnetics. 2006; 27(4):295-306.
  18. Ke Yao, YiBoYu, KaiJun Wang, Juan Ye, DeQiang Lu, Huai Jiang. Absence of effect of power-frequency magnetic fields exposure on mouse embryonic lens development.Bioelectromagnetics. 2007; 28 (8):628-635.
  19. Curcio G, Valentini E, MoroniF,Ferrara M, De GennaroL,Bertini M. Psychomotor performance is not influenced by brief repeated exposures to mobile phones. Bioelectromagnetics. 2008; 29 (3):237-241.
  20. Hae – June Lee, Jeong – Ki Pack, Tae – Hong Kim, Nam Kim, Soo – Yong Choi, Jae – Seon Lee, Sung – Ho Kim, Yun – Sil Lee. The lack of histological changes of CDMA cellular phone – based radio frequency on rat testis. Bioelectromagnetics. 2010; 31 (7):528-534.
  21. Anjana V.Y, David G.K, Janardan K.R.Chapter 17.Cell injury and cellular adaptations. In: Anderson’s Pathology (Ivan Damjanov and James Linder 10th edition). Elsevier Inc.2009; (1): 361-366.
  22. Kamal G.I, Rodney S.M. Chapter 57. Liver In: Anderson’s Pathology (Ivan Damjanov and James Linder 10th edition). Elsevier Inc.2009; (2):1799 – 1822.
  23. Robert E.Anderson, Morgan Berthrong, Louis F.Fajardo. Radiation injury. Anderson’s Pathology. 10th edition. Volume.1.Missouri. Von Hoffman Press.1996:484-512.
  24. Ma HR, Li YY, Luo YP, Ma XL, Gong ZQ. Effect of Guilingji Capsule on the fertility, liver functions, and serum LDH of male SD rats exposed by 900 MHz cell phone. Zhongguo Zhong Xi Yi Jie He Za Zhi. 2014 ; 34(4):475-9.
  25. Mohammed Fathy Assasa. Effect of cellular phone field on body weight, liver enzymes blood indices and role of some antioxidant in albino rats. AAMJ, 2010; 8(3).
  26. Sakai H, Horiguchi N, Endoh D, Nakayama K, Hayashi M. Radiofrequency radiation at 40 kHz induces hepatic injury in Long-Evans Cinnamon (LEC) rats, an animal model for human Wilson disease. J Vet Med Sci. 2011; 73(3):299-304.
  27. Emre M, Cetiner S, Zenier S, Unlukurt I, Kahraman I, Topcu Z. Oxidative stress and apoptosis in relation to exposure to magnetic field. Cell Biochem Biophys.2011; 59:71-77.
  28. Guler G, Seyhan N, Aricioglu A. Effects of static and 50Hz alternating electric fields on superoxide dismutase activity and TBAR’s levels in guinea pigs. Gen Physiol Biophys.2006; 25:177-193.
  29. Zehra Topal, Hatice Hanci, Tolga Mercantepe, Huseyin Serkan Erol, Osman Nuri Keles, Haydar Kaya, Sevdegul Mungan, Ersan Odaci. The effects of prenatal long-duration exposure to 900-MHz electromagnetic field on the 21-day-old newborn male rat liver. Turk J Med Sci. 2015; 45.
  30. F. Oktem, F. Ozguner, H. Mollaoglu, A. Koyu, and E. Uz, “Oxidative damage in the kidney induced by 900-MHz-emitted mobile phone: protection by melatonin,” Archives of Medical Research.2005; 36, (4): 350–355.
  31. Merhan M.R. Effect of exposure and withdrawal of 900MHz electromagnetic waves on brain, kidney and liver oxidative stress and some biochemical parameters in male rats. Electromagn Biol and Med. 2014. DOI:10.3109/15368378.2014.906446.
  32. Martinez S.J, Torres –Duran P.V, Juarez Oropeza M.A. Effects of acute electromagnetic field exposure and movement restraint on antioxidant system in liver, heart, kidney and plasma of Wister rats. A preliminary report. Int. J.Radiat. Biol.2010; 86:1088-1094.

Cite this article: N Mugunthan, J Anbalagan, S Meenachi, S Rajanarayanan. STRUCTURAL CHANGES OBSERVED IN MICE LIVER DUE TO CHRONIC EXPOSURE OF 1900-2200 MHZ RADIATIONS EMITTED FROM MOBILE PHONE. Int J Anat Res 2019;7(1.3):6254-6260. DOI: 10.16965/ijar.2018.438