IJAR.2018.123
Type of Article: Original Research
Volume 6; Issue 2.1 (April 2018)
Page No.: 5091-5095
DOI: https://dx.doi.org/10.16965/ijar.2018.123
STUDY OF SEX DETERMINATION BY PRESENCE OF SEX CHROMATIN IN ORAL MUCOSAL CELLS
Kinjal jethva 1, Ashish Rathva *2, Sanjay chvda 3, S. P Rathod 4.
*1 Assistant professor, SBKS Medical and Research Centre, Waghodia, Vadodara, Gujarat, India.
*2 Tutor, Department of Anatomy, PDU Government Medical College, Rajkot, Gujarat, India.
3 Assistant professor, PDU Government Medical College, Rajkot, Gujarat, India.
4 Dean, GMERS Medical College, Junagadh, Gujarat, India.
Author for correspondence: Dr. Ashish J. Rathva, Tutor, Department of Anatomy, PDU Government Medical College, Rajkot, , Gujarat, India. Contact no. 8758898589. E-Mail: aashish.rathwa60@gmail.com
ABSTRACT:
Introduction: In oral smear, a small condensed mass of sex chromatin usually located just inside the nuclear membrane of the nucleus is called Barr body. The study of Barr bodies is advantageous for sex determination by presence or absent. Oral mucosal smear of 150 students (79 female students and 71 male students in the age group of 17 to 32 years) from P.D.U. Govt. medical college, Rajkot were selected with aim to study oral mucosal cells for presence of sex chromatin in oral mucosal smear and to measure efficacy of oral smear in determination of sex by presence of sex chromatin during 2012 to 2014.
Method: Oral smear was prepared for sex determination by scrapping buccal mucosa with wooden spatula and obtained turbid suspension, these were smeared on a glass slide, fixed by mixture of Methanol and Glacial acetic acid in the ratio of 3:1 for 10 min and stained by Carbol Fuschin for 15-20 min at room temperature, after taking informed written consent from students. Smears were examined with Compound light uniocular microscope under 100x magnification (Oil immersion), cells and nuclei were easily identified.
Observations and Results: One hundred cells were counted in each slide. Mean value of Barr body positive cells in male was 0.647 and Mean value of Barr body positive cells in female was 35.215 and range of percentage of Barr body positive cells in male was 0-5% and range of percentage of Barr body positive cells in female was 0-55%. Presence of sex chromatin in oral mucosal cell of female was statistically significant. (P value < 0.05).
Conclusion: Mean value of Barr body positive cells in male was 0.647 ± 1.148, Mean value of Barr body positive cells in female was 35.215 ± 10.28, range of percentage of Barr body positive cells in female was 0-55% and range of percentage of Barr body positive cells in male was 0-5%. Presence of sex chromatin in oral mucosal cell of female was statistically significant. (P value<0.05) Mean values of sex chromatin positive oral mucosal cells of male was lower than mean value of sex chromatin positive oral mucosal cells in female, that is the supporting fact that sex chromatin is present in female in higher frequency. Sex chromatin can be used as simple and easily available method for sex determination.
Key words: Buccal Mucosa, Microscope, Barr Body.
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