IJAR.2017.184

Type of Article:  Original Research

Volume 5; Issue 2.2 (May 2017)

Page No.: 3800-3804

DOI: https://dx.doi.org/10.16965/ijar.2017.184

EFFECT OF VITAMIN E AGAINST HEAT STRESS INDUCED TESTICULAR DAMAGE IN WISTAR ALBINO RATS

1 Department of Human Anatomy, B P Koirala Institute of Health Sciences, Dharan, Nepal.

2 Department of Human Anatomy, Gandaki Medical College, Pokhara, Nepal.

Corresponding Author: Dr. Janak Raj Awasthi. Department of Human Anatomy, Gandaki Medical College, Pokhara, Nepal. Mobile number: +977 9841781164 E-Mail: janu.awasthi@gmail.com

 ABSTRACT:

Background: Heat stress is one of the most important stressors especially in hot regions of the world. Spermatogenesis and normal testicular function are both temperature dependent. There is compelling evidence that there is a correlation between male infertility and testicular heat stress. Since oxidative stress is the major source of damage after testicular heat stress, it seems logical that antioxidant can prevent germ cell apoptosis and sperm damage. Therefore, the aim of the study was to observe the effect of vitamin E against heat stress induced testicular damage.

Materials and Methods: Thirty-two healthy Wistar Albino rats weighing 130-200gm were randomly divided into 4 groups i.e. group I (control) and group II (vitamin E), III (heat stress), IV (heat stress with vitamin E) each consisting of 8 rats. Rats belonging to group I and II were kept in controlled room temperature of (25±0.5 0 C) and rats of group III and IV were kept in controlled room temperature of (37 ±0.5 0 C) for two weeks. In addition, rats of group II and IV were injected 200mg/kg of vitamin E intraperitoneally. On 15th day all rats were sacrificed and testes was removed and processed for slide preparation and were observed for histological changes. Paired t- test and one way ANOVA were used for data analysis at 95% confidence interval.

Results: Heat stress caused decrease in body weight, testicular weight and size of testes. Histological study showed decrease in diameter and necrosis of epithelial lining of seminiferous tubule in rats under heat stress. Vitamin E showed partially protective effect against heat stress.

Conclusion: Heat stress caused both morphological and histological changes in testes and Vitamin E was partially effective in protecting the testicular damaging effect of heat stress.

Key words: Testes, Heat Stress, Vitamin E, Rats, Light Microscopy and Histology.

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Cite this article: Yadav G, Awasthi J R, Pandey N, Shrestha S, Jha CB. B. EFFECT OF VITAMIN E AGAINST HEAT STRESS INDUCED TESTICULAR DAMAGE IN WISTAR ALBINO RATS. Int J Anat Res 2017;5(2.2):3800-3804. DOI: 10.16965/ijar.2017.184  
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