IJAR.2017.184

Type of Article:  Original Research

Volume 5; Issue 2.2 (May 2017)

Page No.: 3800-3804

DOI: https://dx.doi.org/10.16965/ijar.2017.184

EFFECT OF VITAMIN E AGAINST HEAT STRESS INDUCED TESTICULAR DAMAGE IN WISTAR ALBINO RATS

1 Department of Human Anatomy, B P Koirala Institute of Health Sciences, Dharan, Nepal.

2 Department of Human Anatomy, Gandaki Medical College, Pokhara, Nepal.

Corresponding Author: Dr. Janak Raj Awasthi. Department of Human Anatomy, Gandaki Medical College, Pokhara, Nepal. Mobile number: +977 9841781164 E-Mail: janu.awasthi@gmail.com

 ABSTRACT:

Background: Heat stress is one of the most important stressors especially in hot regions of the world. Spermatogenesis and normal testicular function are both temperature dependent. There is compelling evidence that there is a correlation between male infertility and testicular heat stress. Since oxidative stress is the major source of damage after testicular heat stress, it seems logical that antioxidant can prevent germ cell apoptosis and sperm damage. Therefore, the aim of the study was to observe the effect of vitamin E against heat stress induced testicular damage.

Materials and Methods: Thirty-two healthy Wistar Albino rats weighing 130-200gm were randomly divided into 4 groups i.e. group I (control) and group II (vitamin E), III (heat stress), IV (heat stress with vitamin E) each consisting of 8 rats. Rats belonging to group I and II were kept in controlled room temperature of (25±0.5 0 C) and rats of group III and IV were kept in controlled room temperature of (37 ±0.5 0 C) for two weeks. In addition, rats of group II and IV were injected 200mg/kg of vitamin E intraperitoneally. On 15th day all rats were sacrificed and testes was removed and processed for slide preparation and were observed for histological changes. Paired t- test and one way ANOVA were used for data analysis at 95% confidence interval.

Results: Heat stress caused decrease in body weight, testicular weight and size of testes. Histological study showed decrease in diameter and necrosis of epithelial lining of seminiferous tubule in rats under heat stress. Vitamin E showed partially protective effect against heat stress.

Conclusion: Heat stress caused both morphological and histological changes in testes and Vitamin E was partially effective in protecting the testicular damaging effect of heat stress.

Key words: Testes, Heat Stress, Vitamin E, Rats, Light Microscopy and Histology.

REFERENCES

  1. Lee DHK. Climatic stress indices for domestic animals. Int J Biometeorol. 1965;9(1):29–35.
  2. Stott GH. What is Animal Stress and How is it Measured? J Anim Sci. 1981;52(1):150–3.
  3. Dodd KT, Mundie TG, Lagutchik MS, Morris JR. Cardiopulmonary effects of high-impulse noise exposure. J Trauma. 1997 Oct;43(4):656–66.
  4. Altan O, Pabuçcuoğlu A, Altan A, Konyalioğlu S, Bayraktar H. Effect of heat stress on oxidative stress, lipid peroxidation and some stress parameters in broilers. Br Poult Sci. 2003 Sep;44(4):545–50.
  5. Moreno RD, Alvarado CP. The mammalian acrosome as a secretory lysosome: New and old evidence. Mol Reprod Dev. Wiley Subscription Services, Inc., A Wiley Company; 2006 Nov;73(11):1430–4.
  6. Harrison, R and Weiner J. Abdomino-testicular temperature gradients. J Physiol. 1948;18:256–62.
  7. Waites GM. and SBP. Physiology of mammalian testis. In: Lamming G., editor. Marshall’s Physiology of Reproduction. 2nd ed. New York: Churchill Livingstone; 1990. p. 1–106.
  8. Jung A, Eberl M, Schill WB. Improvement of semen quality by nocturnal scrotal cooling and moderate behavioural change to reduce genital heat stress in men with oligoasthenoteratozoospermia. Reproduction. 2001 Apr;121(4):595–603.
  9. Mieusset R, Bujan L. The potential of mild testicular heating as a safe, effective and reversible contraceptive method for men. Int J Androl. 1994 Aug;17(4):186–91.
  10. Zhu B, Walker SK, Oakey H, Setchell BP, Maddocks S. Effect of paternal heat stress on the development in vitro of preimplantation embryos in the mouse. Andrologia. 2004 Dec;36(6):384–94.
  11. Jung A, Schill W-B, Schuppe H-C. Genital heat stress in men of barren couples: a prospective evaluation by means of a questionnaire. Andrologia. Blackwell Verlag GmbH; 2002 Dec;34(6):349–55.
  12. Paul C, Melton DW, Saunders PTK. Do heat stress and deficits in DNA repair pathways have a negative impact on male fertility? Mol Hum Reprod. 2008 Jan;14(1):1–8.
  13. Collins P, Lacy D. Studies on the structure and function of the mammalian testis. II. Cytological and histochemical observations on the testis of the rat after a single exposure to heat applied for different lengths of time. Proc R Soc London Ser B, Biol Sci. The Royal Society; 1969 Mar;172(1026):17–38.
  14. Kim B, Park K, Rhee K. Heat stress response of male germ cells. Cell Mol Life Sci. 2013 Aug;70(15):2623–36.
  15. Pérez-Crespo M, Pintado B, Gutiérrez-Adán A. Scrotal heat stress effects on sperm viability, sperm DNA integrity, and the offspring sex ratio in mice. Mol Reprod Dev. 2008 Jan;75(1):40–7.
  16. Jannes P, Spiessens C, Van der Auwera I, D’Hooghe T, Verhoeven G, Vanderschueren D. Male subfertility induced by acute scrotal heating affects embryo quality in normal female mice. Hum Reprod. 1998 Feb;13(2):372–5.
  17. Yin Y, Stahl BC, DeWolf WC, Morgentaler A. Heat-induced testicular apoptosis occurs independently of hormonal depletion. Apoptosis. 1998 Sep;3(4):281–7.
  18. Halliwell B, Gutteridge JM, Cross CE. Free radicals, antioxidants, and human disease: where are we now? J Lab Clin Med. 1992 Jun;119(6):598–620.
  19. Thonneau P, Bujan L, Multigner L, Mieusset R. Occupational heat exposure and male fertility: a review. Hum Reprod. 1998 Aug;13(8):2122–5.
  20. Wang Y, Zhao H, Sheng X, Gambino PE, Costello B, Bojanowski K. Protective effect of Fructus Lycii polysaccharides against time and hyperthermia-induced damage in cultured seminiferous epithelium. J Ethnopharmacol. 2002 Oct;82(2-3):169–75.
  21. Gonzalez-Alonso J, Calbet JAL, Nielsen B. Metabolic and thermodynamic responses to dehydration-induced reductions in muscle blood flow in exercising humans. J Physiol. 1999 Oct;520(2):577–89.
  22. Ikeda M, Kodama H, Fukuda J, Shimizu Y, Murata M, Kumagai J, et al. Role of radical oxygen species in rat testicular germ cell apoptosis induced by heat stress. Biol Reprod. 1999;61(2):393–9.
  23. Lucesoli F, Fraga CG. Oxidative stress in testes of rats subjected to chronic iron intoxication and ??-tocopherol supplementation. Toxicology. 1999;132(2-3):179–86.
  24. Gavazza MB, Catala A. The effect of alpha-tocopherol on lipid peroxidation of microsomes and mitochondria from rat testis. Prostaglandins Leukot Essent Fat Acids. 2006;74(4):247–54.

Cite this article: Yadav G, Awasthi J R, Pandey N, Shrestha S, Jha CB. B. EFFECT OF VITAMIN E AGAINST HEAT STRESS INDUCED TESTICULAR DAMAGE IN WISTAR ALBINO RATS. Int J Anat Res 2017;5(2.2):3800-3804. DOI: 10.16965/ijar.2017.184