IJAR.2017.140

Type of Article:  Original Research

Volume 5; Issue 2.1 (April 2017)

Page No.: 3687-3692

DOI: https://dx.doi.org/10.16965/ijar.2017.140

RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) BASED MOLECULAR CHARACTERIZATION OF EVOLUTIONARY ADVANCEMENT AMONGST HIERARCHAL FIVE VERTEBRATE SPECIES

Rajesh B 1, Ramesh B 2, Rajkiran Reddy B 3, Vimala devi N 4, Gayathri KB 5, Bhargav PRK *6.

1 Assistant Professor of Anatomy, Mediciti Institute of Medical Sciences, Hyderabad, India.

2 Assistant Professor of Biochemistry, Mediciti Institute of Medical Sciences, Hyderabad, India.

3 Senior Research Associate, SMART, Sunshine Hospitals, Hyderabad, India.

4 Physician Assistant and Research Scholar, Endocare Hospital, Vijayawada, India.

5 Associate Professor of Gynaecology, PSIMS, Vijayawada, India.

*6 Consultant Endocrine and Metabolic Surgeon, Endocare Hospital, Vijayawada, India.

Address of Corresponding Author: Dr. PRK Bhargav  MS, MCh, FACS, FIMSA, FAIS, Consultant Endocrine and Metabolic Surgeon, Director and Associate Professor of Endocrine Surgery, Endocare Hospital, Vijayawada, India – 520002 Phone No: +91-866-6617633; +91-9490130798
E-Mail: www.drbhargav.org, endoanswers@gmail.com

ABSTRACT

Random Amplified Polymorphic DNA is molecular technique with unique advantage of utility in genetically isolated species without prior genomic information. The prime goal of the present study was to identify molecular characterization and understands the evolutionary advancement among five different vertebrate species. On the basis of the analysis of RAPD profiles amplified by six arbitrary primers that gave the best results in producing species-specific bands (OPG-04, OPG-12, OPG-13, OPG-16, OPG-17 and OPG19), the polymorphic bands were unique in 31.4%, 23.2%, 19.2%,11.3% and 10.8% of rats, avain, gecko, toads and murrels respectively. Based on results, dendrogram constructed for phylogenetic relationship shows wistar rats were most unique and distinct.

Key words: Random Amplified Polymorphic DNA, wistar rat, Gecko, Murrel, Toads, Dendrogram, Phylogeny.

REFERENCES

  1. Bardakci F, Skibinski DOF. Applications of the RAPD technique in tilapia fish: species and subspecies identification. Heredity 1994; 73: 117-123.
  2. Callejas S, Gutiérrez JC. A simple and rapid PCR-based method to isolate complete small macronuclear minichromosomes from hypotrich ciliates: 5S rDNA and S26 ribosomal protein gene of Oxytricha (Sterkiella) nova.  2002 Jun;153(2):133-42.
  3. Chalmers KJ, Waugh R, Sprent JI, Simons A J and Powell W. Detection of genetic variation between and within populations of Gliricidiasepium and G. maculata using RAPD markers.Heredity 1992; 69: 465-472.
  4. Baruffi L, Damiani G, Guglielmino CR, Bandi C, Malacrida AR and Gasperi G. Polymorphism within and between populations of Ceratitiscapitata: comparison between RAPD and multilocus enzyme electrophoresis data. Heredity 1995;71:425-437.
  5. Borowsky RL, McClelland M, Cheng R and Welsh J. Arbitrarily primed DNA fingerprinting for phylogenetic reconstruction in vertebrates: the Xiphophorus model. Mol. Biol. Evol 1995; 12: 1022-1032.
  6. Jobet E, Bougnouxm-E, Morands S, RivaultcC, Cloarec A and Hugot J-P . Use of random amplified polymorphic DNA (RAPD) for generations specific DNA probes for ouxyuroid species (nematode).parasite-journal.org 1998; 5: 47:50.
  7. Kambhampati S, Blavk WC and Rai K S. Random Amplified Polymorphic DNA of mosquito species and populations (Diptera: Culicidae): techniques, statistical analysis, and applications. J. Medical Entomology 1992; 29:939- 945.
  8. Megnegneau B, Debets F and Hoekstra R F. Genetic variability and relatedness in the complex group of black Aspergilli based on random amplified polymorphic DNA. Curr.Genet 1992; 23: 323-329.
  9. Russel J R, Horsein F, Johnson E, Waugh R and Powell W().Genetic differentiation of cocoa (Theobroma cacao L.) populations revealed by RAPD analysis.Molecular Ecology 1993; 2:89-97.
  10. Tibayrenc M, Meubauer K, Barnabe C, Guerrini F, Skarecky D and Ayala FJ. Genetic characterization of six parasitic protozoa: parity between random-primer DNA typing and multilocus enzyme electrophoresis. Proc. Natl. Acad. Sci V;90:1335-1339.
  11. Fani R, Damiani G, Di Serio C, Gallari E, Grifoni A and Bazzicalupa M. Use of random amplified polymorphic DNA (RAPD) for generating specific DNA probes for microorganisms. Molecular Ecology 1993;2: 243- 250.
  12. Bardakci F and Skibinski DOF . Applications of the RAPD technique in tilapia fish: species and subspecies identification. Heredity 1994;73: 117-123.
  13. Naish KA, Warren M, Bardakci F, Skibinski DOF, Carvalho G R and Mair G C.Multilocus DNA fingerprinting and RAPD reveal similar genetic relationships between strains of Oreochromisniloticus (Pisces: Cichlidae). Molecular Ecology 1995; 4:271-274.
  14. Dinesh KR, Lim TM, Chua KL, Chan WK , Phang VPE. RAPD analysis: an efficient method of DNA fingerprinting in fishes. Zoological Science 1993; 10:849-855.
  15. Bardakci F. Applications of the random amplified polymorphic DNA (RAPD) technique in tilapia: species, subspecies and sex identification. Ph.D. Thesis, University of Wales Swansea. 1996.
  16. Beckmann JS and Soller M. Restriction fragment length polymorphisms in genetic improvement: methodologies, mapping and costs. Theor. Appl. Genet 1983; 67:35-43.
  17. Soller M ,Backmann JS. Genetic polymorphism in varietal identification and genetic improvement.Theor. appl. Genet 1983; 67: 25-33.
  18. Smith ML, Bruhn JN, Anderson J B. The fungus Armillariabulbosa is among the largest and oldest living organisms. Nature 1992; 356: 428-43.
  19. Welsh J, Honeycutt R S, McClelland M and Sobral B W S().Parentage determination in maize hybrids using the arbitrarily primed polymerase chain reaction (AP-PCR). Theor. Appl. Genet 1991; 82:473-476.
  20. Hadrys H, Balick M and Schierwater B (). Applications of random amplified polymorphic DNA (RAPD) in molecular ecology.Molecular Ecology, 1: 55-63.
  21. Arnold ML, Buckner CM and Robinson JJ. Pollen mediated introgression and hybrid speciation in Louisiana irises. Proc. Natl. Acad. Sci. USA 1991; 88: 1398-1402.
  22. Riedy MF, Hamilton III WJ , Aquadro C F. Excess of non-parental bands in offspring from known primate pedigrees assayed using RAPD PCR. Nucleic Acids Res 1992; 20:918.
  23. Scott MP, Haymes KM and Williams SM. Parentage analysis using RAPD-PCR. Nucleic Acid Res 1992;20, 5493.
  24. Aldmeida, Miguel AM, Moreira, Cibele R, Bonvicino ,Cerqueira . RAPD Analysis of Nectomys squamies (Rodentia,sigmodontinae) populations. Gentics and molecular biology 2000 ; 23 :4 :793-797.
  25. Rocco L, Valentino IV, Scapigliati G, Stingo V . RAPD-PCR analysis for molecular characterization and genotoxic studies of a new marine fish cell line derived from  Cytotechnology 2014; 66(3): 383–393.
  26. Johnson SL, Midson CN, Ballinger E W and Postlethwait J H. Identification of RAPD primers that reveal extensive polymorphisms between laboratory strains of zebrafish.Genomics 1993;19:152-156.
  27. Bielawski JP, Pumo DE .Randomly amplified polymorphic DNA (RAPD) analysis of Atlantic Coast striped bass. Heredity (Edinb) 19977; 8: Pt 1:32-40.
  28. William . DNA polymorphic bands amphilified by arbitrary primers are useful as genetic markers nucleic acids Res 1990; 25:18 (22):6531-6535.

Cite this article: Rajesh B, Ramesh B, Rajkiran Reddy B, Vimala devi N, Gayathri KB, Bhargav PRK. RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) BASED MOLECULAR CHARACTERIZATION OF EVOLUTIONARY ADVANCEMENT AMONGST HIERARCHAL FIVE VERTEBRATE SPECIES. Int J Anat Res 2017;5(2.1):3687-3692. DOI: 10.16965/ijar.2017.140  
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