IJAR.2017.138

Type of Article:  Original Research

Volume 5; Issue 2.1 (April 2017)

Page No.: 3676-3681

DOI: https://dx.doi.org/10.16965/ijar.2017.138

GENOMICS OF ACETYLCHOLINESTERASE (AChE) ENZYME AND ITS RELATED RNA CONTENT IN THE BRAIN OF RATTUS NORVEGICUS: A PROSPECTIVE STUDY

Rajesh B 1, Ramesh B 2, Rajkiran Reddy B 3, Vimala devi N 4, Gayathri KB 5, Bhargav PRK *6.

1 Assistant Professor of Anatomy, Mediciti Institute of Medical Sciences, Hyderabad, India.

2 Assistant Professor of Biochemistry, Mediciti Institute of Medical Sciences, Hyderabad, India.

3 Senior Research Associate, SMART, Sunshine Hospitals, Hyderabad, India.

4 Physician Assistant and Research Scholar, Endocare Hospital, Vijayawada, India.

5 Associate Professor of Gynaecology, PSIMS, Vijayawada, India.

*6 Consultant Endocrine and Metabolic Surgeon, Endocare Hospital, Vijayawada, India.

Address of Corresponding Author: Dr. PRK Bhargav  MS, MCh, FACS, FIMSA, FAIS, Consultant Endocrine and Metabolic Surgeon, Director and Associate Professor of Endocrine Surgery, Endocare Hospital, Vijayawada, India – 520002, Phone No: +91-866-6617633; +91-9490130798

www.drbhargav.org; endoanswers@gmail.com

ABSTRACT

Inhibition of acetylcholinesterase (AChE), the metabolizing enzyme of acetylcholine is evolving as the most important therapeutic target for development of cognitive enhancers. However, AChE activity in brain has not been properly evaluated on the basis of sex. In the present study, AChE expression was investigated in different brain areas of cerebrum and cerebellum in male and female Rattus norvegicus. On comparing male and female genders, increased AChE activity was seen in cerebrum and cerebellum of female Rattus norvegicus.  However, no significant change in AChE activity was found between cerebrum and cerebellum within the same male and female. Thus it appears that sex alters AChE activity in different brain regions (G4 isoform) that may vary in male and female. Sequence analysis revealed that highest divergence was found in between male cerebrum and female cerebrum (11.9) and least divergence was found in between male cerebellum and female cerebellum (6.4) with control AChE NM_EF534897

Key Words: Acetylcholinesterase, Physiology, Invertebrates, Vertebrates, Cerebrum.

REFERENCES

  1. Boell J, Nechmansohn D. Electron microscopic localization of cholinesterase, by a copperleadthiocholine technique. J Neurochem  1966;3:1345
  2. Aldridge WN, Johnson MK. Cholinesterase, succinic dehydrogenase, nucleic acids, esterase and glutathione reductase in subcellular fractions from rat brain. J Biochem 1959;73:270.
  3. Gerebtzoff MA. Cholinesterases: A histochemical Contribution to the solution of some functional problems. 1959 (Pergemon press, London)1.
  4. Chacho LW,Cerf JA. Histochemical localization of cholinesterase in the amphibian spinal cord and alterations following ventral root section, J Anat.1960;94:74.
  5. Hebb C. Cholinergic neurons in vertebrates, Nature (London). 1961;192:527.
  6. De Robertis E, Pellegraino De Iraldi A, Rodriguez De Lores AG, Salganicoff L. Cholinergic and noncholinergie nerve endings in rat brain. Isolation and subcellular distribution of acetylcholine and acetylcholinesterase. J Neurochem 1962;9:23.
  7. Lewis PR, Shute CD. Confirmation from choline acetylase analyses of a massive cholinergic innervation to the hippocampus. J Physiol 1964;172:9.
  8. Shute CCD, Lewis PR.The fine localization of cholinesterase in the hippocampal formation. J Anat 1965;99:938.
  9. Papp M, Bozsik G. Comparison of the cholinesterase activity in the reticular formation of the lower brain stem of cat & rabbit. J Neurochem 1966;12:697.
  10. Bennett EL, Diamond MC, Morimoto H, Herbert M. Acetylcholinesterase activity and weight measures in fifteen brain areas from six lines of rats. J Neurochem 1966;3:563.
  11. Phillis JW. Acetylcholinesterase in the feline cerebellum. J. Neurochem1968;15:611.
  12. Tripathi A, Srivastva UC. Histoenzymological distribution of acetylcholinesterase in the cerebral hemispheres of Indian wall lizard, Hemidactylus sflaviviridis. Ann Neuro Sci 2007;14:64.
  13. Bhasker KS, Joy KP. Acetylcholinesterase positive intrapineal neuronal system in the palm squirrel Funambulus pennati. Biol struct Morphog 1989;2:7.
  14. Brightman MW, Alberr R.W. Species differences in the distribution of extra neuronal cholinesterase within the vertebrate central nervous system. Neurochem  1959;3:244.
  15. Koelle GB. The histochemical localization of cholinesterases in the central nervous system of the rat. J Comp Neurol 1954;100:211.
  16. Bon S. Molecular forms of acetylcholinesterase in developing Torpedo embryo, Neuro Chem198;2(4):577.
  17. Shen T, Tai, Henchm H, Mc Cammon HA. Molecular dynamics of Acetylcholinesterase, Acc Chem Res 2002;35:332.
  18. Soreq H, Seidman S. Acetylcholinesterase – new roles for an old actor. Nat Rev Neurosci 2001;2:294.
  19. Downes GB, Granto M. Acetylcholinesterase function is dispensable for sensary neurite growth but is critical for neuromuscular synapse stability. Dev Biol 2004;270:232.
  20. Silman I, Sussman JL. Acetylcholinesterase: Classical and nonclassical functions and Pharmacology, Curr opin pharmaco 2005;5:293.
  21. Talesa V, Grauso M, Arpagaus M, Giovannini E, Romani R. Molecular cloning and expression of a full-length cDNA encoding acetylcholinesterase in optic lobes of the squid Loligo opalescens: a new member of the cholinesterase family resistant to diisopropyl fluorophosphates. J. Neurochem 1999;72:1250.
  22. Jones AK, Bentley GN, Parra WG, Agnew A. Molecular characterization of an acetylcholinesterase implicated in the regulation of glucose scavenging by the parasite Schistosoma. The FASEB Journal. 2002 Mar 1;16(3):441-3.
  23. Zhao P, Zhu K.Y, Iang H. Heterologous expression, purification, and biochemical characterization of a greenbug (Schizaphis graminum) acetylcholinesterase encoded by a paralogous gene (ace-1). J Biochem Mol Toxicol 2010;24:51.
  24. Keyhani E, Maigne J. Acetylcholinesterase in mammalian erythroid cells. J. Cell Sci 1981;52:327.
  25. Boudinot E, Bernard V, Camp S, Taylor P, Champagnat J. Influence of differential expression of acetylcholinesterase in brain and muscle on respiration. Respir Physiol Neurobiol 2009;165:40.
  26. Askar KA, Kudi AC, Moody AJ. Purification of Soluble Acetylcholinesterase from Sheep Liver by Affinity Chromatography, Appl Biochem Biotechnol 2011;165:336.
  27. McKenna OC, Angelakos ET. Acetylcholinesterase-containing nerve fibers in the canine kidney. Circ Res 1968;23:645.
  28. El-Bermani AW, Bloomquist EI. Acetylcholinesterase- and norepinephrine- containing nerves in developing rat lung. J Embryol Exp Morphol1978;48:177.
  29. Anguiano GA, Amador A, Moreno-Legorreta ., Arcos-Ortega F, Vazquez- Boucard C. Effects of exposure to oxamyl, carbofuran, dichlorvos, and lindane on acetylcholinesterase activity in the gills of the Pacific oyster Crassostrea gigas. Environ Toxicol 2010;25:327.
  30. Zaitseva OV, Kuznetsova TV. Distribution of acetylcholinesterase activity in the digestive system of the gastropod molluscs Littorina littorea and Achatina fulica. Morfologiia 2008;133:55.
  31. Hornstein EP, Sambursky DL, Chamberlain SC. Histochemical localization of acetylcholinesterase in the lateral eye and brain of Limulus polyphemus: might acetylcholine be a neurotransmitter for lateral inhibition in the lateral eye ? Vis Neurosci 1994;11:989.
  32. Arpagaus M, Richier P, Berge Jb, Toutant Jp. Acetylcholinesterases of the nematode Steinernema carpocapsae. The FEBS Journal. 1992 Aug 1;207(3):1101-8.
  33. Kang JS, Lee DW, Koh YH, Lee SH. A soluble acetylcholinesterase provides chemical defense against xenobiotics in the pinewood nematode. 2001;PLoS One 6: e19063.
  34. von Wachtendonk D, Neef J. Isolation, purification and molecular properties of an acetylcholinesterase (e.c. 3.1.1.7) from the haemolymph of the sea mussel Mytilus edulis, Comp Biochem Physiol 1978;63:279.
  35. Steitz TA, Shulman RG. Crystallographic and NMR studies of the serine proteases, Annu Rev Biophys Bioeng 1982;11:419.
  36. Monnet-Tschudi F, Zurich MG, Schilter B, Costa LG, Honegger P. Maturation-dependent effects of chlorpyrifos and parathion and their oxygen analogs on acetylcholinesterase and neuronal and glial markers in aggregating brain cell cultures. Toxicol Appl Pharmacol 2001;165:175.
  37. Oron U. Acetylcholinesterase and nerve axon formation during muscle regeneration in rats.Cell Mol Biol1984; 30:411.
  38. Das UN. Acetylcholinesterase and butyrylcholinesterase as possible markers of low-grade systemic inflammation. Med Sci Monit 2007;13:214.
  39. Cymborowski B, Skangiel-Kramska J, Dutkowski A. Circadian changes of acetylcholinesterase activity in the brain of house-crickets (Acheta domesticus L). Comp Biochem Physiol 1970;32:367.
  40. Rybicka K. Emryogenesis in Hymenolepis diminuta V. Acetylcholinesterase in embryos. Exp Parasitol 1967;20:263.
  41. Seravin LN. Role of Acetylcholinesterase in Water Metabolism of the „„Dorsal Muscle‟‟ in Leeches. Dokl Akad Nauk SSSR1965; 160:486.
  42. Rand JB. Acetylcholine. 2007 WormBook 1.
  43. Xuereb B, Lefevre E, Garric ., Geffard O. Acetylcholinesterase activity in Gammarus fossarum (Crustacea Amphipoda): linking AChE inhibition and behavioural alteration. Aquat Toxicol 2009;94:114.
  44. Azevedo-Pereira HM, Lemos MF, Soares AM. Effects of imidacloprid exposure on Chironomus riparius Meigen larvae: linking acetylcholinesterase activity to behavior. Ecotoxicol Environ Saf 2001 ;74:1210.
  45. Gibson GE, Peterson C, Jenden DJ. Brain acetylcholine synthesis declines with senescence. Science 1981;213:674.
  46. Silman I, Sussman JL. Structural studies on cholinesterases. In cholinesterases and cholinesterase inhibitors, (Ed) Giacobini E, (Mortin Dunitz, London), 2000:9.
  47. Reiner PB., Fibiger HC. Functional heterogeneity of central cholinergic system, in Psychopharmacology: The Fourth Generation of Progress (Raven Press Ltd, New York) 1995;147.

Cite this article: Rajesh B, Ramesh B, Rajkiran Reddy B, Vimala devi N, Gayathri KB, Bhargav PRK. GENOMICS OF ACETYLCHOLINESTERASE (AChE) ENZYME AND ITS RELATED RNA CONTENT IN THE BRAIN OF RATTUS NORVEGICUS: A PROSPECTIVE STUDY. Int J Anat Res 2017;5(2.1):3676-3681. DOI: 10.16965/ijar.2017.138  
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