IJAR.2016.492

Type of Article:  Original Research

Volume 5; Issue 1 (January 2017)

Page No.: 3415-3420

DOI: https://dx.doi.org/10.16965/ijar.2016.492

MODIFIED LENDRUM’S TECHNIQUE TO DEMONSTRATE METACHROMASIA OF BRAIN SAND IN PINE

Vasudha Kulkarni *1, Ramesh BR 2.

*1 Associate Professor, Department of Anatomy, Dr.B.R. Ambedkar Medical College, Bangalore, Karnataka, India.

2 Professor and Head,   Department of Anatomy, Dr.B.R. Ambedkar Medical College, Bangalore, Karnataka, India.

Address for correspondence:  Dr. Vasudha Kulkarni, Associate Professor, Department of Anatomy, Dr.B.R. Ambedkar Medical College, Bangalore, Karnataka, India. E-Mail: vasuanil77@rediffmail.com

ABSTRACT

Background: Tissue components such as amyloid, mast cell granules and sulfated compounds stain differently from original dye. This is known as metachromasia. Calcium aggregations are known to exhibit metachromasia in certain situations. Local factor which could cause metachromasia is high levels of chondroitin sulfate in tissues undergoing calcification. Pineal gland is one such tissue with calcific concretions in its parenchyma known as Corpora arynecea/ Brain sand.

Objective: The objective of the present study was to demonstrate metachromasia of these calcific concretions using modified Lendrum’s technique.

Methodology and Results: By using modified Lendrum’s technique, metachromasia was demonstrated by corpora arenecea of pineal gland for aqueous methylene blue.

Conclusion: This technique is of importance in histopathology as pineal gland has clinical relevance. The hormone melatonin secreted in excess by pineal gland is associated with depression, peptic ulcers and sexual dysfunction. Melatonin deficiency results in insomnia.

Key words: Pineal gland, Corpora arynecea, Metachromasia, Brain sand.

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Cite this article: Vasudha Kulkarni, Ramesh BR. MODIFIED LENDRUM’S TECHNIQUE TO DEMONSTRATE METACHROMASIA OF BRAIN SAND IN PINEAL GLAND. Int J Anat Res 2017;5(1):3415-3420. DOI: 10.16965/ijar.2016.492
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