IJAR.2016.486

Type of Article:  Original Research

Volume 5; Issue 1 (January 2017)

Page No.: 3389-3393

DOI: https://dx.doi.org/10.16965/ijar.2016.486

STRUCTURAL ABERRATIONS OF ‘Y’ CHROMOSOME IN AZOOSPERMIC MALES

Gajanan Laxmanrao Maske 1, Archana Damdharrao Kannamwar *2.

1 Assistant Professor, Department of Anatomy, Shri. V. N. Government Medical College, Yavatmal, India.

*2 Associate Professor, Department of Anatomy, Shri. V. N. Government Medical College, Yavatmal, India.

Address for Correspondence: Dr. Archana Damdharrao Kannamwar, Shri. V. N. Government Medical College, Yavatmal.445001. India. E-Mail: drgajananlmaske@gmail.com

ABSTRACT 
Background: Male factor infertility is a distressing condition that adds to the psychological trauma to majority of couples. Infertility affects about 15% of all couples attempting pregnancy, with male factor identified in approximately half the cases. One of the major contributing factors of failure of sperm production in testis is genetic disturbance. This can be seen either at chromosomal level or at gene level. Chromosomal abnormalities, numerical or structural can occur in somatic cells (mitotic), testicular germ cells (meiotic) or spermatozoa (gametic).

The context and purpose of the study: 30 non-obstructive Azoospermic infertile males from Acharya Vinoba Bhave Rural Hospital, Sawangi (Meghe), Wardha, were selected for present study. From each subject 3 ml venous blood was collected in a sterile bulb with the help of preheparizined syringes. Chromosomal analysis was carried out by conventional as well as Giemsa Trypsin Giemsa (GTG) technique in cytogenetic laboratory, Department of Anatomy, J. N. Medical College, Sawangi (Meghe), Wardha. For each subject, total 25 metaphases i. e.  15 conventional and 10 G-banded metaphases were analyzed. In cases with chromosomal abnormalities, total 45 metaphases i. e. 25 conventional and 20 G-banded metaphases were studied. Selected metaphases were photographed using CCD camera.

Results: Structural aberration of ‘Y’ chromosome was found in 2 subjects. In both these subjects, the ‘q’ arm of ‘Y’ chromosome was long (46, XYq+) as compare to chromosomes of ‘F’ group. The total percentage of 46, XYq+ in present study comes to 6.67%. This was confirmed by G-banding.

Conclusions:  2 subjects (6.67%)   were detected with long ‘q’ arm of ‘Y’ chromosome (46, XYq+) which compares favorably with literatures on the same subject.

Potential implications: Assisted method of reproduction was an option of treatment for infertile males. The same study could be combined with molecular genetic studies to ascertain the chromosomal anomalies at molecular level and thereby proper counseling could be given to infertile couples. This can be of vital role in planning of parenthood.

Key words: Azoospermia, Metaphase, Y Chromosome, q’ arm.

REFERENCES

  1. Ken McElreavey, Csilla Krausz. Male infertility and Y chromosome. American Jr of Hum Genet 1999;64:928-933.
  2. Singh Amarjeet, Dhaliwal L K and Kaur Arvinder. Infertility in a Primary Health Centre of North India: A follow-up study. The Journal of Family Welfare March 1996;42(1):51-56.
  3. Hargreave T B. In- The management of male infertility. P. G. Publishing Pte. Ltd. Singapore, 1990;1-5.
  4. Okabe M, Ikawa M and Ashkenas J. Gametogenesis ’98. Male infertility and the genetics of spermatogenesis. American journal of human genetics 1998;62:1274-1281.
  5. de Krester D M. Male infertily. The lancet 1997;349:787-790.
  6. Assche E, Bonduelle M, Tournaye H, Joris H, Verheyen G, Devroey P, Steirteghem A and LiebaersI. Cytogenetics of infertile men. Human Reproduction 1996;2(Suppl. 4):1-24.
  7. Elreavey K M, Krause C and Bishop C E. The human Y chromosome and male infertility. Result and Problem in Cell Differention 2000;28:211-232.
  8. Laboratory manual for the examination of human semen and sperm-cervical mucus interaction. IV Edition, Cambridge University Press, UK, 2000.
  9. Nielsen J and Friedrich U. Length of Y chromosome in criminal males. Clinical Genetics 1972;3(4):281-285.
  10. Abramsson L, Beckman G, Duchek M and Nordenson I. Chromosomal aberrations and male infertility. The Journal of Urology, July 1982;128:52-53.
  11. Philip J, Skakkebaek N E, Hammen R, Johnsen S G and Rebbe H. Cytogenetic investigation in male infertility. Acta Obstetrica Gynecologia Scandinavia 1970;49:235-239.
  12. Ghosh P K. Chromosome heteromorphism in children with congenital malformation. Indian Journal of Experimental Biology November 1979;17:1186-1189.
  13. Hendry W F, Polani P E, Pugh RCB, Sommerville I F and Wallace D M. 200 infertile males: correlation of chromosome, histological, endocrine and clinical studies. British Journal of Urology 1976;47:899-908.
  14. Koulischer L and Robert Schoysman. Chromosomes and human infertility. I. Mitotic and Meiotic chromosome studies in 202 consecutive male patients. Clinical Genetics 1974;5:116-126.
  15. Peter J J, Vaidya R A, Rege N R, Gokarn V V, Phadke A G and Ambani L M. Cytogenetic abnormalities in male infertility. Indian Journal of Medical Research February 1980;71: 225-231.
  16. Chandley A C, Edmond P, Christie S, Gowans L, Fletcher J, Frackeiewicz A and Newton M. Cytogenetics and infertility in man. I. Karyotype and seminal analysis. Results of a five-year survey of men attending a sub fertility clinic. Annals of Human Genetics, London 1975;39:31-252.
  17. Micic M, Micic S and Diklic V. Chromosomal constitution of infertile men. Clinical Genetics 1984;25:33-36.
  18. Tarnekar A, Anbalgan J, ghosh S, Fulzele R, Pal A. Cytogenetic study of azoospermic men. Perspective in cytology and genetics 2004;11:443-447.
  19. Court- Brown W M, Jacobs P A and Brunton M. Chromosome studies on randomly chosen men and women. The lancet Sept 1965;561-562.
  20. Muller H, Kinger H P and Glasser M. Chromosome polymorphism in a human newborn population. II. Potentials of polymorphic chromosome variants for characterizing the ideogram of an individual. Cytogenetics and Cell Genetics 1975;15:239-255.
  21. Tiepolo L and Zuffardi O. Localization of factors controlling spermatogenesis in the nonfluorescent portion of the human Y chromosome long arm. Human Genetics 1976;34:119-124.
  22. Foresta Carlo, Moro Enrico and Ferlin Alberto. Y- Chromosome micro deletions and alterations of spermatogenesis. Endocrine Reviews April 2001;22(2):226-239.
  23. Sharpe R M. Life style and environmental contribution to male infertility. British Medical Bulletin 2000;56(3):630-642.

Cite this article: Gajanan Laxmanrao Maske Archana Damdharrao Kannamwar. STRUCTURAL ABERRATIONS OF ‘Y’ CHROMOSOME IN AZOOSPERMIC MALES. Int J Anat Res 2017;5(1):3389-3393. DOI: 10.16965/ijar.2016.486
Share this Research
Share on FacebookShare on Google+Tweet about this on TwitterShare on LinkedInEmail this to someonePrint this pagePin on Pinterest