Type of Article:  Original Research

Volume 10; Issue 3 (September 2022)

Page No.: 8463-8467

DOI: https://dx.doi.org/10.16965/ijar.2022.203

Histo-Functional Improvement of Mammalian Testis after Platelets-Rich Plasma Treatment

Thaer M. Farhan *1, Aiman Al-Maathidy 2.

*1 Associate Professor in Clinical Anatomy and Embryology, Department of Human Anatomy, College of Medicine-Al-Nahrain University, Baghdad, Iraq.

2 Associate Professor, Department of Anatomy and Histology,  College of Medicine, University of Mutah, Karak, Jordan.

Corresponding Author: Dr. Thaer M. Farhan, Associate Professor in Clinical Anatomy and Embryology, Department of Human Anatomy, College of Medicine-Al-Nahrain University, Baghdad, Iraq. Mobile: 00964-790161102 E-Mail: aljomaili2005@gmail.com


Background: Among new therapies emerging in the medical field, the use of platelet-rich plasma (PRP) in human reproduction has not yet been explored. Platelet-rich plasma (PRP) has a potential effect on tissue repair through proliferation and differentiation of tissue progenitor cells. The aim of this study was to evaluate the effect of PRP on the testis structure and function in the rabbit model.

Material and methods: A total of 30 male rabbits were recruited in this study. They were allocated into two groups (15 in each group) to receive an injection of PRP (PRP Group), or normal saline (Control Group)

Results: there were statistically significant differences in Means of germinal layer width, Leydig cell number, and Sertoli cell number was significantly higher in the PRP group compared to that in the control group ( P ≤ 0.05). The PRP group had a higher means of sperm concentration and normal morphology compared with the control groups (P ≤ 0.05).

Conclusion: the platelet-rich plasma is found to have a good potential effect on the testicular tissue that improved the histological and functional aspects and could be considered a promising future treatment for hypogonadism status in many disorders.

Keywords: PRP, reproductive function, testis, Sertoli cells, Leydig cells, the height of the germinal layer

KEY WORDS: Platelet-Rich Plasma (PRP), Tissue Progenitor Cells, Testis.


[1]. Smith, L. B., & Walker, W. H. The regulation of spermatogenesis by androgens. Seminars in cell & developmental biology, 2014;30:2–13.
[2]. Herrera-Alarcón, J., Villagómez-Amezcua, E., González-Padilla, E., & Jiménez-Severiano, H. Stereological study of postnatal testicular development in Blackbelly sheep. Theriogenology, 2007;68(4):582–591.
[3]. Yasser AA., Mahmoud MA. and Gamal KA. Histological and Histomorphometric Changes of the Rabbit Testis During Postnatal Development. Research Journal of Veterinary Sciences. 2012;5(2):42-50.
[4]. Figure cited in https://embryology.med.unsw.edu.au/embryology/images/thumb/a/a9/Testis_histology_2.jpg/300px-Testis_histology_2.jpg cited in https://pin.it/2ihBS4Q January 15, 2022
[5]. Kutluhan MA, Özsoy E, Şahin A, Ürkmez A, Topaktaş R, Toprak T, Gümrükçü G, Verit A. Effects of platelet-rich plasma on spermatogenesis and hormone production in an experimental testicular torsion model. Andrology. 2021;9(1):407-413.
[6]. Lubkowska A, Dolegowska B, Banfi G. Growth factor content in PRP and their applicability in medicine. J Biol Regul Homeost Agents. 2012;26(2 Suppl 1):3S-22S. PMID: 23648195.
[7]. Pavlovic, V., Ciric, M., Jovanovic, V., & Stojanovic, P. Platelet Rich Plasma: a short overview of certain bioactive components. Open medicine (Warsaw, Poland), 2016;11(1):242–247.
[8]. Bader, R., Ibrahim, J. N., Mourad, A., Moussa, M., Azoury, J., Azoury, J., & Alaaeddine, N. Improvement of Human Sperm Vacuolization and DNA Fragmentation Co-Cultured with Adipose-Derived Mesenchymal Stem Cell Secretome: In Vitro Effect. International journal of stem cells, 2019;12(3):388–399.
[9]. Magalon J, Bausset O, Serratrice N, Giraudo L, Aboudou H, Veran J, Magalon G, Dignat-Georges F, Sabatier F. Characterization and comparison of 5 platelet-rich plasma preparations in a single-donor model. Arthroscopy. 2014 May;30(5):629-38. PMID: 24725317.
[10]. Borrione P, Gianfrancesco AD, Pereira MT, Pigozzi F. Platelet-rich plasma in muscle healing. Am J Phys Med Rehabil. 2010;89(10):854-61. PMID: 20855985.
[11]. Ding YQ, Jiang H, Wang CL. ERK and P38MAPK expressions and human sperm motility]. Zhonghua Nan Ke Xue. 2011;17(9):809-12. Chinese. PMID: 21961242.
[12]. Dehghani F, Sotoude N, Bordbar H, Panjeshahin MR, Karbalay-Doust S. The use of platelet-rich plasma (PRP) to improve structural impairment of rat testis induced by busulfan. Platelets.2019;30(4):513-520. PMID: 29883240
[13]. Ribeiro, M. V. M. R., Melo, V. F., Barbosa, M. E. F. C., Tozzi, M. U. F., Ramos, M. S. B., Gaia, N. M. S. R. S., Santos, V. M. G., Neri, W. O., Barbosa, F. T, Ribeiro, A. N. The use of platelet rich-plasma in Ophthalmology: a literature review. Rev Bras Oftalmol., 2017;76(6):319-324.
[14]. Suvarna KS, Layton C., Bancroft JD., (2018). Bancroft’s theory and practice of histological techniques: expert consult. 8th ed. Elsevier. UK.
[15]. Griswold M. D. (1998). The central role of Sertoli cells in spermatogenesis. Seminars in cell & developmental biology, 1998;9(4):411–416.
[16]. Majdic, G., McNeilly, A. S., Sharpe, R. M., Evans, L. R., Groome, N. P., & Saunders, P. T. (1997). Testicular expression of inhibin and activin subunits and follistatin in the rat and human fetus and neonate and during postnatal development in the rat. Endocrinology, 1997;138(5):2136–2147.
[17]. Jegou B. and Skinner MK. (2018) Male Reproduction cited in Encyclopedia of reproduction. 2nd ed. Elsevier. Amsterdam.
[18]. Wijayarathna R., de Kretser D.M., (2016) Activins in reproductive biology and beyond, Human Reproduction Update, 2016;22(3):342–357.
[19]. Halim, B., Angellee, J., Lubis, H.P., Bachsinar, B. Sperm Quality and Deoxyribonucleic Acid Fragmentation after 5 and 10 min Centrifugation with Swim-Up Processing Technique: A Prospective Cohort Study. Open Access Macedonian Journal of Medical Sciences 2012;9:626–630.
[20]. Bo Yan, Yingchun Zhang, Shan Tian, Rui Hu, Bin Wu, (2021). Effect of autologous platelet-rich plasma on human sperm quality during cryopreservation. Cryobiology. 2021;98:12-16.
[21]. Swierstra, E.E. and Foote, R.H. Duration of spermatogenesis and spermatozoan transport in the rabbit based on cytological changes, DNA synthesis and labeling with tritiated thymidine. Am. J. Anat., 1965;116:401-411.

Cite this article: Thaer M. Farhan, Aiman Al-Maathidy. Histo-Functional Improvement of Mammalian Testis after Platelets-Rich Plasma Treatment. Int J Anat Res 2022;10(3):8463-8467. DOI: 10.16965/ijar.2022.203