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IJAR.2020.190

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Type of Article:  Original Research

Volume 8; Issue 3.2 (August 2020)

Page No.: 7703-7706

DOI: https://dx.doi.org/10.16965/ijar.2020.190

EFFECT OF VARIABLE DOSES OF CISPLATIN ON MICROANATOMY OF TESTIS IN ALBINO RATS

Rabiya Amin 1,  Ubaid Ali *2, Maheen Nazir 3.

1 Senior resident, Department of Anatomy, Dr Chandramma Dayananda Sagar Institute Of Medical Education  And Research, Harohalli, Bangalore, Karnataka, India.

*2 Department of Urology, Institute of Nephrourology, Bangalore, Karnataka, India.

3 Senior resident, Department of Anatomy, Govt medical college Srinagar, India.

Corresponding  Author: Dr Ubaid Ali, Department of urology .Institute of Nephrourology Bangalore, Karnataka, India. Phone number -7006560089 E-Mail: dr.ubaidali22@gmail.com

ABSTRACT

Cisplatin is an organic metal co-ordination complex and is  a widely used chemotherapeutic agent against a variety of malignant tumors,  like sarcomas and carcinomas. It interferes with the DNA synthesis and causes apoptosis in view of the fact that the growth of normal cells is also affected. These toxic effects alter the metabolic function of certain tissues and organs. .60 albino rats weighing on an average 100 grams were taken. The animals were divided into three groups. Group A animals received no drug. Group B was injected with 1.3mg/m2 of cisplatin by intraperitoneal route and group C received  2.5mg/m2of cisplatin. The process of drug administration was continued for 12 weeks. The animals were sacrificed in five sittings, after interval of 1,3,6,9 and 12 weeks after drug administration. At the termination of experiments the testis were fixed and stained with H&E. There was degeneration of germ cells with increase in capsular wall thickness of testis, and leydig cell  depletion and sertoli cell depletion in seminiferous tubules. All these findings were prominent in high dose group and progressively increased in severity. Besides this, there was loss of spermatozoa in the lumen of seminiferous tubules in both high and low dose group ,but was profound in high dose group at 12th week. Though cisplatin effectively treats cancer, at an increased dose it has toxic effects to the genitourinary system and other parts of the body.

Key words: Cisplatin, Rat, Testis, Chemotherapeutic agent, Apoptosis, DNA synthesis

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Cite this article: Rabiya Amin, Ubaid Ali, Maheen Nazir. EFFECT OF VARIABLE DOSES OF CISPLATIN ON MICROANATOMY OF TESTIS IN ALBINO RATS. Int J Anat Res 2020;8(3.2):7703-7706. DOI: 10.16965/ijar.2020.190