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IJAR.2020.189

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Type of Article:  Original Research

Volume 8; Issue 3.2 (August 2020)

Page No.: 7692-7702

DOI: https://dx.doi.org/10.16965/ijar.2020.189

THE BIOCHEMICAL AND HISTOLOGICAL CHANGES OF HEPATOCYTES OF ADULT MALE ALBINO RATS FED ON GENETICALLY MODIFIED CORN

Nancy Mohamed Ali El Sekily *1, Dalia Ahmed Esmat Abd El Hamid 1, Fardous Sorour Katb Karawya 2, Amal Abd El Monsef Abo El Magd 1.

*1 Human Anatomy and Embryology Department, Faculty of Medicine, Alexandria University, Egypt.

2 Histology and Cell Biology Department, Faculty of Medicine, Alexandria University, Egypt.

Corresponding Author: Nancy Mohamed Aly El-Sekily, Human Anatomy and Embryology Department, Faculty of Medicine, Alexandria University, Egypt. +0201222359100 E-Mail: nancyelsekily@yahoo.com

ABSTRACT

Introduction: Genetically engineered foods saturate our diet today. A food safety assessment of genetically modified corn has been performed by different researchers on nutrition, allergenicity and toxicology analyses in agreement to the substance equivalence principle, and only few were supported by histopathological confirmations.

Aim: is to study the biochemical and histological changes of hepatocytes of adult male albino rats fed on genetically modified corn.

Materials and methods: Twenty adult male albino rats were randomly classified into two main groups: Group I: (control group) that were given standard diet for three months. Group II: (genetically modified corn group) were fed 50% genetically modified corn and 50% standard diet for three months. At the end of experimental period, analysis of aspartate aminotransferase (AST)/ serum glutamic oxaloacetic transaminase (SGOT) and alanine  aminotransferase (ALT)/ serum glutamic-pyruvic transaminase (SGPT) were done. Fresh specimens were taken from the right lobe of liver of all rats for light microscopic examination, Gomori’s trichrome stain, Best’s carmine stain, Aoyama’s stain and Electron microscopy.

Results: In the current work, the mean of ALT and AST in group (II) was significantly increased compared to that of control group. Light microscopic examination of the histological sections of group II showed, diffuse affection of the hepatic lobules. Gomori’s trichrome revealed newly formed collagen fibers in the portal tract. Best’s carmine stain showed diffuse increase in glycogen granules in the majority of hepatocytes. Aoyama’s stain revealed dilatation and proliferation of bile canaliculi. Electron microscopic examination revealed evident ultra-structural changes.

Key Words: Genetically modified corn (GMC), Hepatocytes, Albino Rats.

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Cite this article: Nancy Mohamed Ali El Sekily, Dalia Ahmed Esmat Abd El Hamid, Fardous Sorour Katb Karawya, Amal Abd El Monsef Abo El Magd. THE BIOCHEMICAL AND HISTOLOGICAL CHANGES OF HEPATOCYTES OF ADULT MALE ALBINO RATS FED ON GENETICALLY MODIFIED CORN. Int J Anat Res 2020;8(3.2):7692-7702. DOI: 10.16965/ijar.2020.189