Type of Article:  Original Research

Volume 7; Issue 3.1 (July 2019)

Page No.: 6775-6779

DOI: https://dx.doi.org/10.16965/ijar.2019.221


Ankit Jain 1, Prateek Shrivastava *2, Rashmi Jain 3.

1 M.S., Assistant Professor, Department of Anatomy, Shyam Shah Medical College, Rewa (M.P.), India.

*2 Resident, Department of Anatomy, Mahatma Gandhi Memorial Medical College, Indore (M.P.), India.

3 M.D., Assistant Professor, Department of Pathology, Shyam Shah Medical College, Rewa (M.P.), India

Corresponding author: Dr. Prateek Shrivastava, Resident, Department of Anatomy, Mahatma Gandhi Memorial Medical College, Indore (M.P.), India. Telephone: +91 9827570908. E-Mail: ankitjain6285@gmail.com


Introduction: Fetal capability to grow in utero depends on placental development and function. The goal of this study was to appraise the effects of hypertension on placental weight and Wharton’s jelly area (WJA); and correlate them in normal and pre-eclamptic pregnancies.

Material and Methods: Eighty placentae along with umbilical cord divided into forty each of normotensive and pre-eclamptic pregnancies were studied. The cross-sectional area of the umbilical cord and vessels area was measured with the help of vernier scale and ocular micrometer respectively. WJA was calculated by deduction of the vascular area from the umbilical cord area. Placental weight was recorded by using a weighing machine and correlated with the WJA.

Results: In the present study, mean placental weight was 445.45 ± 40.31 grams and WJA was 35.28 ± 8.42 mm2 in the normal group. Whereas, in the pre-eclamptic group, mean placental weight was 408.95 ± 47.15 grams and WJA was 29.04 ± 8.09 mm2. Mean placental weight and WJA was significantly lower in the pre-eclamptic group. A significant positive correlation was found between WJA and placental weight (r = 0.710, p<0.0001) in normal group and (r = 0.764, p<0.0001) in pre-eclamptic group.

Conclusion: Pre-eclampsia is associated with reducing placental weight and WJA. Low WJA may hamper the fetal growth. The current study shows a strong positive correlation between WJA and placental weight. So, the awareness of this correlation will be helpful in the early observation of placental insufficiencies and provide sufficient information to take additional care in such conditions.

Key words: Wharton’s jelly, Placental weight, Pre-eclampsia, Umbilical cord.


  1. Teasdale F. Gestational changes in the functional structure of the human placenta in relation to foetal growth: a morphometric study. American Journal of Obstetrics and Gynecology 1980; 137(5):560-8.
  2. Das B, Dutta D, Chakraborthy S, Nath P. Placental morphology in hypertensive disorders of pregnancy and its correlation with fetal outcome. J Obstet Gynecol India. 1996; 46(1):40-6.
  3. Wang HS, Hung SC, Peng ST, Huang CC, Wei HM, Guo YJ, Fu YS, Lai MC, Chen CC. Mesenchymal stem cells in the Wharton’s jelly of the human umbilical cord. Stem cells. 2004 Dec 1; 22(7):1330-37.
  4. Vizza E, Correr S, Goranova V, Heyn R, Muglia U, Papagianni V. The collagen fibrils arrangement in the Wharton’s jelly of full-term human umbilical cord. Italian journal of anatomy and embryology.1995; 100:495- 501.
  5. Filiz AA, Rahime B, Keskin HL, Esra AK. Positive correlation between the quantity of Wharton’s jelly in the umbilical cord and birth weight. Taiwanese Journal of Obstetrics and Gynecology. 2011 Mar 31; 50(1):33-6.
  6. Sobolewski K, Bankowski E, Chyczewski L et al. Collagen and glycosaminoglycans of Wharton’s jelly. Biol Neonate 1997; 71:11–21.
  7. Sakamoto T, Ono H, Saito Y. Electron microscopic histochemical studies on the localization of hyaluronic acid in Wharton’s jelly of the human umbilical cord [in Japanese]. Nippon Sanka Fujinka Gakkai Zasshi 1996; 48:501– 507.
  8. Goodlin RC. Fetal dysmaturity,“lean cord,” and fetal distress. American journal of obstetrics and gynecology. 1987 May 31; 156(5):1357.
  9. Bankowski E, Palka J, Jaworski S. Preeclampsia is associated with alterations in insulin-like growth factor (IGF)-1 and IGF-binding proteins in Wharton’s jelly of the umbilical cord. Clinical chemistry and laboratory medicine. 2000 Jul 11; 38(7):603-8.
  10. Ali AF, Fateen B, Ezzet A, Badawy H, Ramadan A, El-tobge A. Lack of proteoglycans in Wharton’s jelly of the human umbilical cord as a cause of unexplained fetal loss in diabetic infants. Obstetrics & Gynecology. 2000 Apr 30; 95(4):S61-62.
  11. Galewska Z, Bańkowski E, Romanowicz L, Gogiel T, Wolańska M, Jaworski S. Preeclampsia-associated reduction of cathepsin D activity in the umbilical cord. Clinica chimica acta. 2005 Jan 31; 351(1):177-84.
  12. Barnwal M, Rathi SK, Chhabra S, et al. Histomorphometry of umbilical cord and its vessels in pre-eclampsia as compared to normal pregnancies. Nepal Journal of Obstetrics & Gynaecology. 2012; 7(1):28-32.
  13. Inan S, Sanci M, Can D, et al. Comparative morphological differences between umbilical cords from chronic hypertensive and preeclamptic pregnancies. Acta Medica Okayama. 2002; 56(4):177-86.
  14. Park K. Park’s textbook of Preventive and Social Medicine. 22nd Jabalpur: M/S Banarsidas Bhanot India; 2013.
  15. Udainia A, Jain ML. Morphological study of placenta in pregnancy induced hypertension with its clinical relevance. J Anat Soc India. 2001; 50(1):24-7.
  16. Krishna U, Bhalerao S. Placental insufficiency and fetal growth restriction. The Journal of Obstetrics and Gynecology of India. 2011 Oct 1; 61(5):505-11.
  17. Singh S, Gugapriya TS. A cross sectional morphometric study of hypertensive with normal placentae and its correlation with fetal outcome. Int J Anat Res. 2014; 2(2):437-42.
  18. Majumdar S, Dasgupta H, Bhattacharya K, Bhattacharya A. A study of placenta in normal and hypertensive pregnancies. J. Anat. Soc. India. 2005; 54(2):1-9.
  19. Nanaev AK, Kohnen G, Milovanov AP, Domogatsky SP, Kaufmann P. Stromal differentiation and architecture of the human umbilical cord. Placenta. 1997 Jan 31; 18(1):53-64.
  20. Klein J, Meyer FA. Tissue structure and macromolecular diffusion in umbilical cord immobilization of endogenous hyaluronic acid. Biochimica et Biophysica Acta (BBA)-General Subjects. 1983 Feb 22; 755(3):400-11.
  21. Di Naro E, Ghezzi F, Raio L, Franchi M, D’Addario V. Umbilical cord morphology and pregnancy outcome. European Journal of Obstetrics & Gynecology and Reproductive Biology. 2001 Jun 30; 96(2):150-157.
  22. Tavares FJ, Lourdes PM. Coarctation of the umbilical cord: a cause of intrauterine fetal death. International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics. 1977; 15(5):469-73.

Cite this article: Ankit Jain, Prateek Shrivastava, Rashmi Jain. HISTOMORPHOMETRIC ASSESSMENT OF WHARTON’S JELLY AREA IN NORMAL AND PRE-ECLAMPTIC PREGNANCIES AND ITS CORRELATION WITH PLACENTAL WEIGHT. Int J Anat Res 2019;7(3.1):6775-6779. DOI: 10.16965/ijar.2019.221