IJAR.2018.350

Type of Article:  Original Research

Volume 6; Issue 4.1 (October 2018)

Page No.: 5820-5829

DOI: https://dx.doi.org/10.16965/ijar.2018.350

SELENIUM MODULATES HEPATORENAL DAMAGE AND GENOTOXICITY INDUCED BY POTASSIUM DICHROMATE IN ADULT ALBINO RATS

Asmaa Mohammed Tolba *1, Samia Hussein 2.

*1 Anatomy and Embryology Department, Faculty of medicine, Zagazig University, Egypt .

2 Medical Biochemistry and Molecular Biology Department, Faculty of Medicine, Zagazig University, Egypt.

The corresponding authors’ address: Dr. Asmaa Mohammed Tolba, Anatomy and Embryology Department, Faculty of medicine, Zagazig University, 44519, Egypt. tel: 002 01142203098 E-Mail : amtolba@zu.edu.eg

ABSTRACT:

Background: Potassium dichromate (K2Cr2O7) is a major environmental pollutant and is known for its wide toxic manifestations. The present study shows the protective role of selenium (Na2SeO3) against chromium induced hepatorenal toxicity.

Materials and Methods: Sixty  adult albino rats were divided into four groups of fifteen each. Group I is control group which received standard diet; group II received K2Cr2O7 (67mg/kg B.W) in drinking water; group III received selenium only (0.5 μg/kg B.W) and group IV received both K2Cr2O7 (67mg/kg B.W) and selenium (0.5 μg/kg B.W) for 6 weeks.

Results: Selenium supplementation to the group IV improved all the kidney and liver chemical function parameters. In addition, it down-regulates endoplasmic reticulum stress genes.

Conclusion: The biochemical results confirmed the improvement of histopathological findings. Therefore, our study revealed that selenium  was effective in preventing K2Cr2O7-induced hepatorenal toxicity.

Key words: Selenium, hepatorenal, Potassium dichromate, PCR, Rats.

REFERENCES

  1. Arreola-Mendoza L., J.L. Reyes, E. Melendez, D. Martin, M.C. Namorado, E. Sanchez, L.M. Del Razo,Alpha-tocopherol protects against the renal damage caused by potassium dichromate.Toxicology, 2006;218: 237–246.
  2. O’Brien, T.J., Ceryak, S., Patierno, S.R.Complexities of chromium carcinogenesis: role of cellular response, repair and recovery mechanisms. Mutat Res, 2003;533, 3–36.
  3. Xu XR, Li HB, Li XY, Gu JD. Reduction of hexavalent chromium by ascorbic acid in aqueous solutions. Chemosphere, 2004; 57: 609–13.
  4. Zhitkovich A. Chromium: exposure, toxicity and biomonitoring approaches. In biomarkers of environmentally associated disease: technologies, concepts, and perspectives. CRC Press, 2002;p. 269–87.
  5. Pedraza-Chaverri J., D. Barrera, O.N. Medina-Campos, R.C. Carvajal, R. Hernandez- Pando, N.A. Macias-Ruvalcaba, P.D. Maldonado, M.I. Salcedo, E. Tapia, L. Saldivar, M.E. Castilla, M.E. Ibarra-Rubio, Time course study of oxidative and nitrosative stress and antioxidant enzymes inK2Cr2O7-induced nephrotoxicity. 2005; BMC Nephrol. 64.
  6. Bosgelmez II, Soylemezo˘glu T, Guvendik G.The protective and antidotal effects of taurine on hexavalent chromium-induced oxidative stress in mice liver tissue. Biol Trace Elem Res,2008;125:46–58.
  7. Fatima S, Mahmood R.Vitamin C attenuates potassium dichromate-induced nephrotoxicity and alterations in renal brush border membrane enzymes and phosphate transport in rats. Clin Chim Acta, 2007; 386: 94–99.
  8. Bagchi D, Balmoori J, Bagchi M, Ye X,Williams CB, Stohs SJ. Comparative effects of TCDD endrin, naphthalene and chromium (VI) on oxidative stress and tissue damage in the liver and brain tissues of mice. Toxicology, 2002;175:73–82.
  9. Ueno S, Kashimoto T, Susa N, Furukawa Y, Ishii M, Yokoi K, et al.Detection of dichromate (VI)-induced DNA strand breaks and formation of paramagnetic chromium in multiple mouse organs. Toxicol Appl Pharmacol, 2001;170:56–62.
  10. Eisler R.Selenium handbook of chemical risk assessment: health hazards to humans, plants, and animals. vol. 3. Boca Raton, FL: CRC Press;. 2000; p. 1649–705.
  11. Tinggi U. Selenium: its role as antioxidant in human health. Environ Health Prev Med 2008;13:102–8.
  12. Soudani Nej , Mediha Sefi , Ibtissem Ben Amara,Tahia Boudawara , Boudawara B, Najiba Zeghal. Protective effects of Selenium (Se) on Chromium (VI) induced nephrotoxicity in adult rats. Ecotoxicology and Environmental Safety 2010;73:671-678.
  13. Ferri KF, Kroemer G. Organelle-specific initiation of cell death pathways. Nat Cell Biol 2001;3: E255–E263.
  14. Ron D.Translational control in the endoplasmic reticulum stress response. J Clin Invest 2002;110: 1383–1388.
  15. Nakagawa T, Zhu H, Morishima N, Li E, Xu J , Yankner BA, Yuan J. Caspase-12 mediates endoplasmic reticulum specific apoptosis and cytotoxicity by amyloid- β. Nature 2000;403: 98–103.
  16. Bancroft J, Gamble M. Theory and Practice of Histological Techniques, 6th ed. Churchill Living-stone, Scotland, London 2008; pp 125-138.
  17. Sun Y.,G. Liu, T.Song, F.Liu, W. Kang, Y. Zhang, Z.Ge. Upregulation of GRP78 and caspase-12 in diastolic failing heart. Acta Biochimica Polonica 2008;55(3), 511–516.
  18. Plaa LG, Hewitt RW (1997) Toxicology of the liver, 2nd ed. In: Target Organ Toxicology Series. Washington, DC: Taylor & Francis.PP431.
  19. Acharya UR, Mishra M, Tripathy RR, Mishra I.Testicular dysfunction and antioxidative defense system of Swiss mice after chromic acid exposure. Reprod Toxicol, 2006; 22:87–91.
  20. Banu SK, Samuel JB, Arosh JA, Burghardt RC, Aruldhas MM. Lactational exposure to hexavalent chromium delays puberty by impairing ovarian development, steroidogenesis and pituitary hormone synthesis in developing Wistar rats.Toxicol Appl Pharmacol, 2008; 232:180–9.
  21. Barrera, P.D. Maldonado, O.N. Medina-Campos, R. Hernandez- Pando, M.E. Ibarra-Rubio, J. Pedraza-Chaverri. HO-1induction attenuates renal damage and oxidativestressinducedby K2Cr2O7. Free Radical Biol Med 2003;34: 1390–1398.
  22. Kotyzov D ,Hodkov A ,Bludovsk M ,Eybl V. Effect of chromium (VI) exposure on antioxidant defense status and trace element homeostasis in acute experiment in rat. Toxicol Ind Health .Toxicology and Industrial Health 2013; 2, 31(11) 1044–1050.
  23. Soudani Nej , Mediha Sefi , Ibtissem Ben Amara , Tahia Boudawara , Najiba Zeghal.Effects of selenium on chromium (VI)-induced hepatotoxicity in adult rats Exp Toxicol Pathol 2011; 63 541– 548.
  24. Kalayarasan S, Sriram N, Sureshkumar A, Sudhandiran G.Chromium (VI)-induced oxidative stress and apoptosis is reduced by garlic and its derivative Sallylcysteine through the activation of Nrf2 in the hepatocytes of Wistar rats. J Appl Toxicol, 2008; 28: 908–19.
  25. Shi L, Wenjuan XUN, Wenbinen YUE, Chunxiangh ZHANG, Youshe REN, Lei  SHI, etal Effect of sodium selenite, Se-yeast and nano-elemental selenium on growth performance, Se concentration and antioxidant status in growing male goats. Small Rumin Res, 2011; 96:49–52.
  26. Jin, S. Zhang, R. Tao, J. Huang, X. He, L. Qu, Z. Fu.Oral Exposure of Mice to Cadmium (II), Chromium (VI) and Their Mixture Induce Oxidative- and Endoplasmic Reticulum-Stress Mediated Apoptosis in the Livers. Environ Toxicol, 2016; 31: 693–705.
  27. Liu H, Li X, Qin F, Huang K. Selenium suppresses oxidative-stress-enhanced vascular smooth muscle cell calcification by inhibiting the activation of the PI3K/AKT and ERK signaling pathways and endoplasmic reticulum stress. J Biol Inorg Chem, 2014; 19(3):375–388.
  28. Karaca S, Eraslan G.The effects of flaxseed oil on cadmium-induced oxidative stress in rats. Biol Trace Elem Res, 2013;155(3):423–430.
  29. Sinha K, Das J, Pal PB,Sil PC. Oxidative stress: the mitochondria-dependent and mitochondria-independent pathways of apoptosis. Arch Toxicol 2013;87(7):1157–1180.
  30. Walte P, Ron D. The unfolded protein response: from stress pathway to homeostatic regulation. Science 2011; 334(6059):1081-1086.
  31. Lee AS. The ER chaperone and signaling regulator GRP78/BiP as a monitor of endoplasmic reticulum stress. Methods, 2005;35(4): 373-381.
  32. Xia Q, Feng X, Huang H, Du L, Yang X,Wang K.Gadolinium-induced oxidative stress triggers endoplasmic reticulum stress in rat cortical neurons. J Neurochem 2011; 117(1):38–47.
  33. Ron D, Walter P. Signal integration in the endoplasmic reticulum unfolded protein response. Nat Rev Mol Cell Biol 2007; 8(7): 519–529.

Cite this article: Asmaa Mohammed Tolba, Samia Hussein. SELENIUM MODULATES HEPATORENAL DAMAGE AND GENOTOXICITY INDUCED BY POTASSIUM DICHROMATE IN ADULT ALBINO RATS. Int J Anat Res 2018;6(4.1):5820-5829. DOI: 10.16965/ijar.2018.350