IJAR.2018.107

Type of Article:  Original Research

Volume 6; Issue 1.3 (March 2018)

Page No.: 5041-5045

DOI: https://dx.doi.org/10.16965/ijar.2018.107

KLINGLER’S FIBER DISSECTION METHOD- AN ASSISTING APPROACH TOWARDS TEACHING NEUROANATOMY

Sachin patil 1, Madhu sethi *2, Sabita Mishra 3.

1 Assistant professor, Department of Anatomy, ANIIMS, Port Blair, A&N Islands, India.

*2 Assistant professor, Department of Anatomy, BSA Medical college and Hospital, Delhi, India.

3 Director Professor & Head, Department of Anatomy, Maulana Azad medical college, New Delhi, India.

Corresponding Author Dr Madhu Sethi, Assistant Professor, Department of Anatomy, BSA Medical College and Hospital, Rohini, Delhi, India-110085 E-Mail: madhusethi567@gmail.com

ABSTRACT:

Background and Objective: The Klingler fiber dissection technique is a simple and less complicated method for identifying the fine structure of the white fiber tracts of brain. In this study, we have used classical fiber dissection technique by Klinger’s to produce white matter specimens which can be used for explaining anatomy of various white matter tracts to students.

Materials and Methods: Five brains specimen removed from formalin fixed human cadavers (3 males and 2 female) were used in this study. Klinger’s fibers dissection method was used to obtain white fibers specimen. Dissection of the cerebrum was performed using wooden spatulas, fine curved metal spatulas, fine forceps. The white fibers were exposed by peeling brain with help of wooden spatula to expose the fibers. The dissection microscope was used to isolate small structures.

Results: Using the classical Klinger’s technique, we were able to obtain a brain specimen depicting organization of various white fibres such as corona radiata, superior longitudinal bundle, association fibres with fibres passing in relation to lentiform nucleus. In another specimen, dissection of right cerebral hemisphere medial to lentiform nucleus showed continuity of white projection fibres of corona radiata as internal capsule. Fibres of corpus callosum were delineated in two specimens which displayed spatial disposition of its various parts.

Conclusion: White matter fiber of brain are very important for understanding of function of the central nervous system function. The Klingler’s fiber dissection technique with other study material can successfully serve the purpose of the teaching of complex brain architecture of white matter. These dissected specimens will be more attractive to students, than the mere imagination of white fiber tracts during neuroanatomy classes.

Key words: Corona radiate, Fiber dissection; Klingler technique, Lentiform nucleus, Corpus callosum.

REFERENCES

  1. Familiari G, Relucenti M, Heyn R, Baldini R, D’Andrea G, Familiari P, Bozzao A, Raco A. The value of neurosurgical and intraoperative magnetic resonance imaging and diffusion tensor imaging tractography in clinically integrated neuroanatomy modules: A cross-sectional study. Anat Sci Educ 2013;6:294–306.
  2. Klingler J. Erleichterung der makroskopischen Praeparation des Gehirns durch den Gefrierprozess. Schweiz Arch Neurol Psychiatr 1935;36:247–256.
  3. Ludwig E, Klingler J. 1956. Atlas Cerebri Humani: Der innere Bau des Gehirns dargestellt auf Grund mackroskopischer Praeparate. Basel, Switzerland: S. Karger. 1956;136.
  4. Klingler J, Gloor P. The connections of the amygdala and of the anterior temporal cortex in the human brain. J Comp Neurol 1960;115:333–369.
  5. Moon K, Filis AK, Cohen AR. The birth and evolution of neuroscience through cadaveric dissection. Neurosurgery 2010;67:799–809.
  6. Collins JP. Modern approaches to teaching and learning anatomy. BMJ 2008;337:665–667.
  7. Estevez ME, Lindgren KA, Bergethon PR. A novel three-dimensional tool for teaching human neuroanatomy. Anat Sci Educ 2010;3:309–317.
  8. Ciccarelli O, Catani M, Johansen-Berg H, Clark C, Thompson A. Diffusion- based tractography in neurological disorders: Concepts, applications, and future developments. Lancet Neurol 2008; 7:715–727.
  9. Schmahmann JD, Smith EE, Eichler FS, Filley CM. Cerebral white matter: Neuroanatomy, clinical neurology, and neurobehavioral correlates. Ann N Y Acad Sci 2008; 1142:266–309.
  10. Dimou S, Battisti RA, Hermens DF, Lagopoulos J. 2013. A systematic review of functional magnetic resonance imaging and diffusion tensor imaging modalities used in presurgical planning of brain tumour resection. Neurosurg Rev 2013;36:205–214.
  11. Arnts, M. Kleinnijenhuis, J.G. Kooloos, A.N. Schepens-Franke, A.M. van Cappellen van Walsum,;Combining fiber dissection, plastination, and tractography for neuroanatomical education: Revealing the cerebellar nuclei and their white matter connections, Anat Sci Educ 2014;7:47-55.
  12. Khot Z, Quinlan K, Norman GR, Wainman B. The relative effectiveness of computer-based and traditional resources for education in anatomy. Anat Sci Educ 2013;6:211–215.
  13. Ture U, Yasargil MG, Friedman AH, Al-Mefty O. Fiber dissection technique: Lateral aspect of the brain. Neurosurgery 2000;47:417–426.
  14. Agrawal A, Kapfhammer JP, Kress A, Wichers H, Deep A, Feindel W, Sonntag VK, Spetzler RF, Preul MC. Josef Klingler’s models of white matter tracts: Influences on neuroanatomy, neurosurgery, and neuroimaging. Neurosurgery 2011;69:238–252.
  15. L. Kier, L.H. Staib, L.M. Davis, R.A. Bronen,;1; Anatomic dissection tractography: a new method for precise MR localization of white matter tracts, AJNR Am J Neuroradiol 2004;25:670-676.
  16. Zemmoura, I., E. Blanchard, P. I. Raynal, C. Rousselot-Denis,C. Destrieux, and S. Velut. How Klingler’s Dissection Permits Exploration of Brain Structural Connectivity?. An electron microscopy study of human white matter: Brain Struct. Funct. 2015;221:2477–2486.

Cite this article: Sachin patil, Madhu sethi, Sabita Mishra. KLINGLER’S FIBER DISSECTION METHOD- AN ASSISTING APPROACH TOWARDS TEACHING NEUROANATOMY. Int J Anat Res 2018;6(1.3):5041-5045. DOI: 10.16965/ijar.2018.107