Type of Article:  Original Research

Volume 5; Issue 2.1 (April 2017)

Page No.: 3687-3692

DOI: https://dx.doi.org/10.16965/ijar.2017.140


Rajesh B 1, Ramesh B 2, Rajkiran Reddy B 3, Vimala devi N 4, Gayathri KB 5, Bhargav PRK *6.

1 Assistant Professor of Anatomy, Mediciti Institute of Medical Sciences, Hyderabad, India.

2 Assistant Professor of Biochemistry, Mediciti Institute of Medical Sciences, Hyderabad, India.

3 Senior Research Associate, SMART, Sunshine Hospitals, Hyderabad, India.

4 Physician Assistant and Research Scholar, Endocare Hospital, Vijayawada, India.

5 Associate Professor of Gynaecology, PSIMS, Vijayawada, India.

*6 Consultant Endocrine and Metabolic Surgeon, Endocare Hospital, Vijayawada, India.

Address of Corresponding Author: Dr. PRK Bhargav  MS, MCh, FACS, FIMSA, FAIS, Consultant Endocrine and Metabolic Surgeon, Director and Associate Professor of Endocrine Surgery, Endocare Hospital, Vijayawada, India – 520002 Phone No: +91-866-6617633; +91-9490130798
E-Mail: www.drbhargav.org, endoanswers@gmail.com


Random Amplified Polymorphic DNA is molecular technique with unique advantage of utility in genetically isolated species without prior genomic information. The prime goal of the present study was to identify molecular characterization and understands the evolutionary advancement among five different vertebrate species. On the basis of the analysis of RAPD profiles amplified by six arbitrary primers that gave the best results in producing species-specific bands (OPG-04, OPG-12, OPG-13, OPG-16, OPG-17 and OPG19), the polymorphic bands were unique in 31.4%, 23.2%, 19.2%,11.3% and 10.8% of rats, avain, gecko, toads and murrels respectively. Based on results, dendrogram constructed for phylogenetic relationship shows wistar rats were most unique and distinct.

Key words: Random Amplified Polymorphic DNA, wistar rat, Gecko, Murrel, Toads, Dendrogram, Phylogeny.


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Cite this article: Rajesh B, Ramesh B, Rajkiran Reddy B, Vimala devi N, Gayathri KB, Bhargav PRK. RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) BASED MOLECULAR CHARACTERIZATION OF EVOLUTIONARY ADVANCEMENT AMONGST HIERARCHAL FIVE VERTEBRATE SPECIES. Int J Anat Res 2017;5(2.1):3687-3692. DOI: 10.16965/ijar.2017.140