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Ravi
Bhaskar,
Doshi.M.A,
Shakunthala.R.Pai,
Ravishankar.B.
EVALUATION
OF
HISTOPATHOLOGICAL
AND
BIOCHEMICAL
CHANGES
OF
TAMRA
BHASMA
(A
COPPER
BASED
MINERAL
FORMULATION)
REPEATED
DOSE
TOXICITY
STUDY
IN
WISTAR
ALBINO RATS.
Int J Anatomy Res 2016;4(4):3246-3252. DOI: 10.16965/ijar.2016.452.
Type of Article: Original Research
DOI: http://dx.doi.org/10.16965/ijar.2016.452
Page No.: 3246-3252
EVALUATION
OF
HISTOPATHOLOGICAL
AND
BIOCHEMICAL
CHANGES
OF
TAMRA
BHASMA
(A
COPPER
BASED
MINERAL
FORMULATION) REPEATED DOSE TOXICITY STUDY IN WISTAR ALBINO RATS
Ravi Bhaskar *
1
, Doshi.M.A
2
, Shakunthala.R.Pai
3
, Ravishankar.B
4
.
*1
PhD Scholor, Anatomy Department, Krishna Institute of Medical Sciences Deemed University, Karad, Maharashtra, India.
2
Prof & HOD, Anatomy Department, Krishna Institute of Medical Sciences Deemed University, Karad, Maharashtra, India.
3
Prof
&
HOD,
Anatomy
Department,
Srinivas
Institute
of
Medical
Sciences
&
Research
Centre,
Mukka,
Mangalore,
Karnataka,
Director
4
,
SDM
Research
centre for Ayurveda and Allied Sciences, Udupi, Karnataka.
Corresponding Author:
Ravi Bhaskar, Department of Anatomy, Srinivas Institute of Medical Sciences and Research Centre, Mukka, Mangaluru, India.
E-Mail:
drravibhaskarr@gmail.com
ABSTRACT
Objective:
The
objective
of
this
present
study
was
to
evaluate
safety
profile
of
Tam
ra
Bhasma
with
main
emphasis
on
biochemical
and
histopathological
changes in Wistar albino rats.
Study
design:
The
toxicity
study
was
done
by
repeated
dose
on
Wistar
albino
rats.
Animals
were
made
into
three
different
categories
or
groups
which
contains
ten
rats
in
each
group.
Group
I
administered
with
0.5%
CMC
&
considered
as
vehicle
control.
Group
II
&
III
administered
with
Tamra
Bhasma
1.98mg/kg
&
9mg/kg
respectively.
These
groups
were
administered
the
specific
drug
Tamra
Bhasma
for
28
consecutive
days.
On
28
th
day
after
one
hour
of
last dosing, animals were sacrificed.
Data sources and extraction:
The blood and important organs were subjected for biochemical and histopathological examinations.
Results:
The
repeated
administration
of
Tamra
Bhasma
didn’t
produce
remarkable
toxicity
at
therapeutic
dose
level.
However
Tamra
Bhasma
at
five
times
of
therapeutic
dose
produced
changes
in
biochemical
parameters
such
as
there
is
an
increase
in
the
serum
creatinine
level.
This
was
without
concomitant
increase
in
serum
urea
level-
which
was
found
to
be
decreased.
Total
bilirubin
level
was
found
to
be
elevated.
Kidney
sections
from
Tamra
Bhasma
administered
at
5
times
of
TED
has
shown
necrotic
changes
in
the
tubular
epithelium,
vacuolization
oedema
in
the
interstial
tissue
and
focal
cell
infiltration
however,
the
cyto-architecture
of
the
other
important
organs
were
not
affected
in
a
drastic
manner.
Conclusion:
The
repeated
administration
of
test
drugs
at
therapeutic
dose
level
confirms
their
relative
safety;
however
at
high
dose
level
they
may
have
some
concern
related
to
renal
functioning
and
hence
the
benefits of repeated administration of
Tamra Bhasma
outweighed the toxic potential.
KEY WORDS: Tamra Bhasma, Histopathology, Serum urea, Total bilirubin, Creatinine, Tubular epithelium.
References
1
.
Gogtay, Bhatt N J, Dalvi S S, The use and safety of non-allopathic Indian medicine, Drug saf.2002;25:1005-1019
2
.
O’ Dell B L, Sunde R A. Hand book of Nutritionally essential mineral elements, Marcel Dekkar Inc. New York, 1997, p.680.
3
.
Vagbhata
.
Rasa Ratna Samuchchaya
.
Ambika datta Shastri
, editor, Varanasi: Chowkamba Sanskrit Bhawan, 1
st
edition, 1988; P.99. pp.622
4
.
Vagbhata
.
Rasa Ratna Samuchchaya
.
Ambika datta Shastri
, editor, Varanasi: Chowkamba Sanskrit Bhawan, 1
st
edition, 1988; P.101. pp.622
5
.
Mookerjee
Bhudeb.
Rasajala
nidhi
–
Ocean
of
Indian
Chemistry,
Vol.
2.
Medicine
and
Alchemy.
4th
edition.
Delhi:
Chaukhambha
Orientalia;
2004.
P.279.pp.288.
6
.
Mishra Siddinandana.
Ayurvediya Rasashastra
, Varanasi: Chaukhambha Orientalia, Revised edition 2009;P.94.pp.610
7
.
Satpute AD, Ratna Samuchaya of Vagbatta (Translation), Chowkamba Sanskrit pratishtana, Varanasi, 2003.
8
.
Sonali
Dhamal,
Mrudhula
P
Wadekar,
Kulkarni
B
A,
Dhapte
V
V.
Chemical
Investigations
of
Some
Commercial
Samples
of
Calcium
Based
Ayurvedic
Drug of Marine Origin: Journal of Pharmacy and Biological Sciences. 2013;6:5-12.
9
.
Kumar
A,
Nair
A
G
C,
Reddy
A
V,
Garg
A
G,
“
Bhasmas
:
unique
Ayurvedic
metallic-herbal
preparations,
chemical
characterization,”
Biological
Trace
Element Research
. 2006;109:231–254.
1
0
.
Pondey BL, A study of the effect of Tamra Bhasma on experimental Gastric ulcers and secretion. Ind. J. Exp. Biol. 1983;21:258-264.
1
1
.
Paget
G
E,
Barnes
J
M.
Evaluation
of
drug
activities.
In:
Lawrence
DR,
Bacharach
AL,
editors.
Pharmacometrics.Vol.
1.
New
York:
Academic
press;
1964.
p. 161.
1
2
.
Ayush
Guidelines:
170
Guidelines
for
Evaluation
of
Ayurveda,
Siddha
and
Unani
drugs
&
Other
Traditional
Medicines
of
India,
Published
in
the
Gazette
of India on. 2008.
1
3
.
Bancroft J D, Gamble M. Theory and practice of histological techniques. 5th ed. Queen's Medical Centre, Nottingham, UK; Churchill Livingstone
1
4
.
Leslie P. Gartner. James L. Hiatt, Colour text book of histology, 3
rd
Ed; Elsevier publication, 2009; pp: 236-248.
1
5
.
Mescher, Junqueira’s Histology; Text & Atlas, with correlated cell and molecular biology, 6
th
Ed, Lippincott Williams & Wilkins, Philadelphia; 2011.
1
6
.
Ross, Michael H & Pawlina, wojeioch Histology, a text and atlas; Harper international edition, New York; 1985
1
7
.
Parmar
MY,
Shah
PA,
Thakkar
VT,
Gandhi.
TR.
Hepatoprotective
activity
of
A.
Subulatum
Roxb
seed
against
ethanol-induced
liver
damage
in
rats.
Int
J
Green Pharm. 2009;2:250-54.
1
8
.
Wilkinson
J
H,
Boutwell
J
H,
Winsten
S.
Evaluation
of
a
new
system
for
kinetic
measurement
of
serum
alkaline
phosphatase.
Clin
Chem.
1969;15:487-
95.
1
9
.
Chmidt
E,
Schemidt
F
W,
Mohr
J,
Otto
P,
Vido
I,
Wrongeman
K,
Herfarth
C
(eds.).
In:
Pathogenesis
and
mechanism
of
liver
cell
necrosis.
4th
ed.
Lancester, Medical and Technical Publications, 1975, p 147.
2
0
.
Bradly DW, Maynard JE, Emery G, Webster H. Transaminase activities in serum of long-term hemodialysis patients. ClinChem. 1972;18:1442.
2
1
.
Rajesh M J, Latha M S, Hepatoprotection by
Elephantopus scaber
Linn. in CCL4-induced liver injury. Indian J Physiol.Pharmacol. 2001;45:481-86.
2
2
.
Anonymous,
International
Commission
for
protectionagainst
environmental
mutagen
and
carcinogens
report
of
committee.
Mutat
Res,
1983;114:120-
77.
2
3
.
Dixon MF, Nimmo J, Prescott LF. Experimental paracetamol - induced hepatic necrosis: A histopathological study. J Pathol. 1971: 103; 225-27.
24.
Leslie P. Gartner. James L. Hiatt, colour text book of histology, 3
rd
Ed; Elsvier publication, 2009; pp: 236-248.