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Shweta
Jha,
Dinesh
Kumar,
JM
Kaul.
EFFICACY
OF
IMMEDIATE,
24
HOURS
VERSUS
48
HOURS
CULTURE
METHOD
IN
CYTOGENETIC
STUDY
OF
PEDIATRIC
ACUTE
LYMPHOBLASTIC
LEUKEMIA.
Int
J
Anatomy
Res
2016;4(4):3175-3178.
DOI:
10.16965/ijar.2016.432.
Type of Article: Original Research
DOI: http://dx.doi.org/10.16965/ijar.2016.432
Page No.: 3175-3178
EFFICACY OF IMMEDIATE, 24 HOURS VERSUS 48 HOURS CULTURE METHOD IN CYTOGENETIC STUDY OF PEDIATRIC ACUTE LYMPHOBLASTIC LEUKEMIA
Shweta Jha *
1
, Dinesh Kumar
2
, JM Kaul
3
.
*
1
. Senior Resident, Department of Anatomy, University College of Medical Sciences, Delhi, India.
2
Professor, Department of Anatomy, Maulana Azad Medical College, Delhi, India.
3
Consultant Professor, Dr Baba Saheb Ambedkar Medical College, Delhi, India.
Correspondence
address:
Dr.
Shweta
Jha,
Department
of
Anatomy,
University
college
of
medical
sciences,
Dilshad
Garden,
Delhi
110095,
India.
Phone:
9654173164
E-Mail:
jha350@gmail.com
ABSTRACT
Background:
Leukemia
is
the
most
common
childhood
cancer
in
India
with
relative
proportion
varying
between
25
and
40%.
60
to
85%
of
all
leukemias
reported
are
acute
lymphoblastic
leukemia
(ALL).Karyotyping
is
a
technique
that
provides
critical
diagnostic
and
prognostic
information
that
allows
the
delivery of appropriate treatment. Success rate depends on sample conditions, type of disease and culture conditions.
Aim:
To compare efficacy of culture methods at varying times in karyotyping for genetic abnormalities of Acute Lymphoblastic Leukemia.
Materials
and
Methods:
Bone
marrow
aspirates
(0.6
ml)
after
adding
15
ml
RPMI
medium
were
divided
into
three
parts
for
immediate(direct)
culture,
24
hour culture and 48 hour culture method , were incubated according to their respective time duration and karyotyping was done.
Result:
In
immediate
culture,
there
were
very
few
small
sized
cells
with
no
metaphases.
In
24
hour
culture,
cells
had
a
moderate
population
with
no
analysable metaphases. In 48 hour culture, cell population was high with bigger sized cells and 20 or more metaphases were observed per slide.
Conclusion:
48 hours culture method is better than immediate and 24 hour culture in ALL patients.
KEY WORDS:
Acute Lymphoblastic Leukemia (ALL), Bone Marrow, Karyotyping, Culture.
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