International Journal of Anatomy and Research

Welcome to International Journal of Anatomy and Research




Type of Article: Original Research

Year: 2016 | Volume 4 | Issue 3 | Page No. 2854-2859

Date of Publication: 30-09-2016



Hina Nafees *1, Satyam Khare 2, Shilpi Jain 3, Gautam Sarkar 4.

*1 Assistant Professor, Department of Anatomy, TMU, Moradabad, India.

2 Professor, Department of Anatomy, Subharti Medical College, Meerut, India.

3 Professor, Department of Anatomy, Subharti Medical College, Meerut, India.

4 Professor and Head, Department of Biochemistry, Subharti Medical College, Meerut, India.

Address: Dr. Hina Nafees, Assistant Professor, Department of Anatomy, TMU, Moradabad, India. Contact no. 9761684179.


Introduction:  T2D affects over 340 million people worldwide. T2D affects low – and middle – income populations accounting for more than 80% of the deaths due to diabetes. The genetic variants associated with T2D are complicated by lifestyle and environmental factors that play a major role in disease onset and progression. This research work is an attempt to contribute in the search for genes responsible for complex forms of T2DM.
Objective of the Study: Our study explores the association of SDF- 1β between genetic variants and T2DM in North Indian Population.
Materials and Methods:  This study was retrospective, analytical case control study & was carried out in the Central Research lab of Subharti Medical College. Meerut. Peripheral blood was collected from 150 unrelated T2DM patients attending Out – patient and In-patient departments of medicine C.S.S.H Meerut. India for diabetes & 150 healthy controls. Whole venous blood was collected & used for DNA extraction. DNA was further amplified by the technique of polymerase chain reaction (PCR). Two sets of primers were used in PCR i-e forward and reverse primer. These fragments were further subjected to the digestion by restriction endonuclease enzyme Msp1 and were subjected to agarose gel electrophoresis.
Results: The odd ratio of SDF -1ß genotypes and alleles were calculated in T2DM and compare them with control subjects. The frequency of SDF -1 ß G / G homozygous wild in T2DM showed a significantly high relative risk, when compared with healthy controls. Relative risk associated with this genotype was 4.500. p value was calculated to be < 0.0001.
Conlusion: On the basis of our study we can conclude that The “GG” genotype of SDF-1β gene is confined to be the risk allele and is associated with T2DM patients in North Indian population.  Although this study focused on a relatively small number of individuals, its finding contribute to the growing evidence of the presence and effects of genetic variant in the understudied North Indian population.
Key Words: T2DM, SDF- 1β gene, Genotypes, Alleles.


  1. Dunstan DW, Zimmet PZ, Welborn TA, De Courten MP, Cameron AJ, Sicree RA et al. The rising prevalence of diabetes and impaired glucose tolerance: the Australian Diabetes, Obesity and Lifestyle Study. Diabetes Care.2002;25:829-834.
  2. Mohan V, Deepa M, Deepa R, Shanthirani CS, Farooq S, Ganesan A, et al.Secular trends in the prevalence of diabetes and impaired glucose tolerance in urban South India: the Chennai Urban Rural Epidemiology Study (CURES-17): Diabetologia. 2006;49:1175–1178.
  3. Ramachandran A, Snehalatha C, Kapur A, Vijay V, Mohan V, Das AK, et al. High prevalence of diabetes and impaired glucose tolerance in India: National Urban Diabetes Survey. Diabetologia 2001;44:1094–1101.
  4. Gu D, Reynolds K, Duan X, Xin X, Chen J, Wu X, Mo J, Whelton PK, He J. Prevalence of diabetes and impaired fasting glucose in the Chinese adult population: International Collaborative Study of Cardiovascular Disease in Asia (InterASIA): Diabetologia. 2003; 46:1190–1198.
  5. Shaw JE, Sicree RA, Zimmet PZ. Global estimates of the prevalence of diabetes for 2010                                                     and 2030. Diabetes Res Clin Pract. 2009;87:4-14.
  6. Das KVK. Textbook of medicine. New Delhi: Medical Publishers. 2002;4:429-46.
  7. Bertin E, Arner P, Bolinder J, Toft EH. Action of glucagon and glucagon – like petide -1- (7 - 36) amide on lipolysis in human subcutaneous adipose tissue and skeletal muscles in vivo. The Journal of Clinical Endocrinology and Metabolism. 2001;86(3):1229-34.
  8. Dumonteil E, Magnan C, Ritz’ Laser B, Meda P, Dussoix P, Gilbert M, Ktorza A, Phillipe J. Insulin but not glucose lowering corrects the hyperglucagonemia and increased proglucagon messenger ribonucleic acid levels observed in insulinopenic diabetes. Endocrinology. 1998;139(11):4540-6.
  9. Unger RH. Diabetes and the alpha cell. Diabetes. 1976;25:136-51.
  10. D. Rossi, A. Zlotnik , The biology of chemokines and their receptors, Annu, Rev. Immunol. 2000;18:217-242 .
  11. A. Zlotnic, O. Yoshie , chemokines a new classification system and their role in immunity, Immunity. 2000;12:121-127.
  12. Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes: Estimates for the year 2000 and projections for 2030. Diabetes Care. 2004;27:1047-53.
  13. Derakhshan R, Arababadi MK, Ahmadi Z, et al. Increased Circulating Levels of SDF-1 (CXCL12) in Type 2 Diabetic Patients Are Correlated to Disease State but Are Unrelated to  Polymorphism of the SDF-1β Gene in the Iranian Population. Inflammation. 2012;  35(3):900-4.
  14. Vijay Viswanathan, Dhamodharan Umapathy, Ezhilarasi Krishnamoorthy et al. Stromal Cell-derived Factor (SDF-1 β) Gene Single Nucleotide Polymorphism at Position G801A is Associated with Type 2 Diabetes Mellitus in a South Indian Population”, International Journal of Genetic Engineering. 2013;3(1):1-5.
  15. Chaudhary O, Rajsekara K, Ahmeda I, et al. Polymorphic variants in DC-SIGN, DC- SIGN and SDF-1 in high risk seronegative and HIV-1 patients in Northern Asian Indians. J Clin Virol. 2008;43(2):196-201.
  16. Vairaktaris E, Vylliotis A, Spyridonodou S, et al. A DNA Polymorphism of Stromal-derived Factor-1is Associated with Advanced Stages of Oral Cancer. Anticancer Res. 2008;28(1A):271-5. 


Hina Nafees, Satyam Khare, Shilpi Jain, Gautam Sarkar. ASSOCIATION OF SDF-1 GENE SINGLE NUCLEOTIDE POLYMORPHISM IN TYPE 2 DIABETES MELLITUS PATIENTS IN NORTH INDIAN POPULATION. Int J Anat Res 2016;4(3):2854-2859. DOI: 10.16965/ijar.2016.355




Volume 1 (2013)

Volume 2 (2014)

Volume 3 (2015)

Volume 4 (2016)

Submit Manuscript