Type of Article: Original Research
Year: 2016 | Volume 4 | Issue 3 | Page No. 2854-2859
Date of Publication: 30-09-2016
ASSOCIATION OF SDF-1ß GENE SINGLE NUCLEOTIDE POLYMORPHISM IN TYPE 2 DIABETES MELLITUS PATIENTS IN NORTH INDIAN POPULATION
Hina Nafees *1, Satyam Khare 2, Shilpi Jain 3, Gautam Sarkar 4.
*1 Assistant Professor, Department of Anatomy, TMU, Moradabad, India.
2 Professor, Department of Anatomy, Subharti Medical College, Meerut, India.
3 Professor, Department of Anatomy, Subharti Medical College, Meerut, India.
4 Professor and Head, Department of Biochemistry, Subharti Medical College, Meerut, India.
Address: Dr. Hina Nafees, Assistant Professor, Department of Anatomy, TMU, Moradabad, India. Contact no. 9761684179.
Introduction: T2D affects over 340 million people worldwide. T2D affects low – and middle – income populations accounting for more than 80% of the deaths due to diabetes. The genetic variants associated with T2D are complicated by lifestyle and environmental factors that play a major role in disease onset and progression. This research work is an attempt to contribute in the search for genes responsible for complex forms of T2DM.
Objective of the Study: Our study explores the association of SDF- 1β between genetic variants and T2DM in North Indian Population.
Materials and Methods: This study was retrospective, analytical case control study & was carried out in the Central Research lab of Subharti Medical College. Meerut. Peripheral blood was collected from 150 unrelated T2DM patients attending Out – patient and In-patient departments of medicine C.S.S.H Meerut. India for diabetes & 150 healthy controls. Whole venous blood was collected & used for DNA extraction. DNA was further amplified by the technique of polymerase chain reaction (PCR). Two sets of primers were used in PCR i-e forward and reverse primer. These fragments were further subjected to the digestion by restriction endonuclease enzyme Msp1 and were subjected to agarose gel electrophoresis.
Results: The odd ratio of SDF -1ß genotypes and alleles were calculated in T2DM and compare them with control subjects. The frequency of SDF -1 ß G / G homozygous wild in T2DM showed a significantly high relative risk, when compared with healthy controls. Relative risk associated with this genotype was 4.500. p value was calculated to be < 0.0001.
Conlusion: On the basis of our study we can conclude that The “GG” genotype of SDF-1β gene is confined to be the risk allele and is associated with T2DM patients in North Indian population. Although this study focused on a relatively small number of individuals, its finding contribute to the growing evidence of the presence and effects of genetic variant in the understudied North Indian population.
Key Words: T2DM, SDF- 1β gene, Genotypes, Alleles.
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Hina Nafees, Satyam Khare, Shilpi Jain, Gautam Sarkar. ASSOCIATION OF SDF-1ß GENE SINGLE NUCLEOTIDE POLYMORPHISM IN TYPE 2 DIABETES MELLITUS PATIENTS IN NORTH INDIAN POPULATION. Int J Anat Res 2016;4(3):2854-2859. DOI: 10.16965/ijar.2016.355