Type of Article: Original Research
Year: 2016 | Volume 4 | Issue 3 | Page No. 2547-2551
Date of Publication: 31-07-2016
MICROSCOPIC STUDY OF SKELETAL MUSCLE IN EMBALMED CADAVERS
Archana Kalyankar *1, Shivaji Sukre 2, Santoshkumar Dope 1, Sanjog Pahade 3.
*1 Associate Professor, Department Of Anatomy, Government Medical College, Aurangabad, Maharashtra, India.
2 Professor and Head, Department Of Anatomy, Government Medical College, Aurangabad, Maharashtra, India.
3 House Officer, Department Of Anatomy, Government Medical College, Aurangabad, Maharashtra, India.
Address: Dr. Archana G. Kalyankar, Associate Professor, Department Of Anatomy, Government Medical College, Aurangabad, Maharashtra, India. Mobile No.: +919975133601
Introduction: Histology slides are prepared from animals like guinea pigs, mice etc. routinely for the purpose of teaching and research. In the department of Anatomy, cadavers are available for dissection purpose. Here, an attempt has been made to prepare the histology slides from human cadaveric tissue obtained after embalming. The aim of present study was designed to obtain skeletal muscle sample from human cadavers after embalming for the purpose of preparation of slide. The study was undertaken to see the feasibility of prepared skeletal muscle slide from human cadaver for teaching purpose and to enhance the research activity.
Materials and Methods: Twelve human cadavers received to the department of anatomy through body donations were selected for sample collection. The taken tissues were fixed into 10% buffered formalin, processed and stained with usual H and E staining. The prepared slide was studied and compared with the skeletal muscle slide of the guinea pig.
Results: Post embalmed skeletal sections are better in staining and appearance as that of guinea pig slides.
Conclusion: The present study was undertaken on muscle tissue with H and E staining was found to be feasible for histological study, but slides from other tissues from human cadavers by using special stains needs to be studied. There is also scope to obtain normal tissues removed from living human beings during surgical procedures for preparing histology slides after taking proper consent but needs further study of its feasibility.
KEY WORDS: Skeletal Muscle, Cadaver, Embalming.
- Gupta T. and Gauba K. Cadaveric tissue histology: A viable alternative. Journal of clinical and diagnostic research 2011 DEC;5(8):1505-1509.
- Williams P L; Gray’s Anatomy, 38th edition, Churchill Livingstone, Edinburgh; 1995: pp 1592-93.
- Inderbir Singh. Textbook of Human Histology; sixth edition:(123to132).
- Judy A. and Provoklimek. A novel method of preparing histology slides to integrate the teaching of gross and microscopic anatomy: J. vet. Med. Educ. 2002; (fall)29(3): 137-41.
- Andrew W. and Susan W. Histopathology from the dissecting room: are cadavers a suitable source of educationally useful histopathology specimen. International journal of experimental and clinical anatomy 2015;9(1):26-33.
- Nicholson H.D. and Samalia L. Comparison of different embalming fluid on the quality of histological preservation in human cadavers. Eur J morpho. 2005 oct-dec;42(4-5):178-84
- Uchenna N. andEkele J. Effect of embalming fluid on histological appearance of organs from embalmed based African boat cadavers. Histologic June 13; vol XLVI No. 1:1-16.
- Alexander C.B. and Luiz A.U. Histological study of fresh vs. frozen semitendinous muscle tendon allograft. Clinics 2010;65(3):297-303.
- Richter G.T., Smith J.E., Spencer H.J., Fan C.Y., Vural E. Histological comparison of implanted cadaveric and porcine dermal matrix grafts. Pubmed: Otolaryngol Head Neck Surg. 2007 AUG; 137(2):239-42.
Archana Kalyankar, Shivaji Sukre, Santoshkumar Dope, Sanjog Pahade. MICROSCOPIC STUDY OF SKELETAL MUSCLE IN EMBALMED CADAVERS. Int J Anat Res 2016;4(3):2547-2551. DOI: 10.16965/ijar.2016.270