Type of Article: Original Research
Year: 2016 | Volume 4 | Issue 2 | Page No. 2216-2221
Date of Publication: 30-04-2016
AN ALTERNATIVE APPROACH TO BONE CLEANING METHODS FOR ANATOMICAL PURPOSES
Navita Aggarwal *1, Monika Gupta 2, Parmod Kumar Goyal 3, Jaswinder Kaur 4.
*1 Associate Professor, Department of Anatomy, Adesh Institute of Medical Sciences and Research, Bathinda, Punjab, India.
2 Associate Professor, Department of Anatomy, Adesh Institute of Medical Sciences and Research, Bathinda, Punjab, India.
3Professor and Head, Department of Forensic Medicine, Adesh Institute of Medical Sciences and Research, Bathinda, Punjab, India.
4 Professor and Head, Department of Anatomy, Adesh Institute of Medical Sciences and Research, Bathinda, Punjab, India.
Address: Dr. Navita Aggarwal, Associate Professor, Department of Anatomy, Adesh Institute of Medical Sciences and Research, Bathinda, Punjab, India.
Mobile no.: +919855424777
Introduction: Many methods of bone cleaning have been mentioned and followed by various experts over period of years. Most of them are tedious and time consuming. Also they need training and expertise of persons doing these procedures. In this study we have tried to modify some of the older methods to make the extraction and cleaning of bones easy and less time consuming. Also we expect that the bones extracted by this method will be more presentable for study. Thus the preparation of bones specimens by this method can be of immense value and time saver for many research institutes to get their self prepared bone specimens and thus help them in becoming self sufficient.
Materials and Methods: Lots of the donated bodies under the ongoing body donation programme are being used in the department of Anatomy, AIMSR. After dissection and autopsy in the dissection hall, the cadavers which have been buried for a period of about 1 year were dug for the remains. The bones were collected with the help of technicians and washed thoroughly with tap water. The bones were then put in varying concentration of H2O2 and observed for desired changes.
It was also recorded that how much time did each concentration take to clean the bone of its soft tissue. Also the amount of bleaching/whitening of bone will be noticed. Care was taken that none of the original features of bone were lost or attenuated. If at lower concentration some of the tissue was not cleaned off the bones, then higher concentration of hydrogen peroxide were tried.
Conclusion: Finally we were able to conclude that at what concentration the bones clean easily and at earliest without damage and with required amount of bleaching. They were then dipped in solution of fixative for preventing damage and giving them a presentable look.
KEY WORDS: Bones, Cleaning, Hydrogen Peroxide.
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Navita Aggarwal, Monika Gupta, Parmod Kumar Goyal, Jaswinder Kaur. AN ALTERNATIVE APPROACH TO BONE CLEANING METHODS FOR ANATOMICAL PURPOSES. Int J Anat Res 2016;4(2):2216-2221. DOI: 10.16965/ijar.2016.181