Type of Article: Original Research
Year: 2016 | Volume 4 | Issue 1 | Page No. 1961-1966
Date of Publication: 29-02-2016
EFFECT OF CENTELLA ASIATICA LEAVES EXTRACT ON HIPPOCAMPAL CA3 NEURONS: A MORPHOLOGICAL STUDY IN MICE
Hemamalini *1, Rao M.S 2.
*1 Assistant professor, Dept of Anatomy, JSS Medical College, JSS University, Mysore, Karnataka, India.
2 Department of Anatomy, Faculty of Medicine, Kuwait University, P.O. Box 24923, 13110, Kuwait.
Address: Dr. Hemamalini, Assistant Professor, Department of Anatomy, Jagadguru Sri Shivarathreeshwara Medical College, Mysore-570015, Karmataka, India. Phone number: +918762357259, Fax number: +91 821-2548345, 2493819
Introduction:Centella asiatica is a perennial herbaceous creeper, growing in moist soil. It has anti-thrombotic, antibacterial and antiinflammatory properties. In Ayurvedic medicine, Centella asiatica is used extensively as brain tonic to enhance the neural function, learning and memory. In the present study, the neuroprotective role of Centella asiatica (CeA) leaves extract on hippocampal CA3 neurons against stress induced neurodegeneration in mice was investigated.
Materials and Methods: Three months old albino mice were divided into four groups and treated as follows: (i) Normal control (NC) - remained without any treatment, (ii) Saline control (SC) - treated with saline, (iii) Stressed group (S) - stressed in a wire mesh restrainer for 6 hours/day for 6 weeks, (iv) Stress + CeA treated group (S+CeA) - stressed and treated orally with CeA leaves extract throughout the stress period (n=6 in all groups). At the end of the experimental period, mice in all groups were anesthetised, perfused transcardially with 50ml of saline followed by 100ml of 10% formalin. Brain was removed and processed for paraffin sections, sections were stained with cresyl violet stain. Number of neurons in the hippocampal CA3 region was counted in ten randomly selected fields (1000μ2area each).
Results: Neurons in the hippocampal CA3 region, S group was found to be shrunken, irregular in shape with pyknotic nuclei compared to NC and SC groups. However such neurodegenerative changes were not seen in S+CeA group. Number of neurons in S group was significantly less compared to control groups and S+CeA group (P<0.001).
Conclusion: Oral intubation of Centella asiatica leaves extract can protect the hippocampal CA3 neurons from degeneration in stressed mice.
Key words: Restraint stress, Hippocampal CA3 neurons, Neurodegeneration.
- Bliss TV, Collingridge GL. A synaptic model of memory: long-term potentiation in the hippocampus. Nature 1993;361:31-9.
- Knigge KM, Hays M. Evidence of inhibitive role of hippocampus in neural regulation of ACTH release. Proc Soc Exp Biol Med 1963;114:67-9.
- Hyman BT, Van Hoesen GW, Damasio AR, Barnes CL. Alzheimer's disease: cell specific pathology isolates the hippocampal formation. Science 1984;225:1168-70
- Slovitier RS. Epiletptic brain damage in rats induced by sustained electrical stimulation of the perforant path. 1. Acute electrophysiological and light microscopic studies. Brain Res Bull 1983;10:765-97.
- Shukla SD, Jain S, Sharma K and Bhatnagar M. Stress induced neuron degeneration and protective effects of Semicarpus anacardium Linn. and Withania somnifera Dunn. in hippocampus of albino rats: an ultrastructural study. Indian J Exp Biol.2000;38(10):1007-1013.
- Saxena G, Mamta singh, Meena P, Barber S, Sharma D, Shukla S, Bhatnagar M. Neuroprotective effects of asparagus racemosus linn root extract: An experimental and clinical evidence. Annals of Neurosciences. 2007,14(3).
- Mohandas Rao KG, Muddanna Rao S, Gurumadhva Rao S. Centella asiatica (L.) leaf extract treatment during the growth spurt period enhances hippocampal CA3 neuronal dendritic arborization in rats. Evid Based Complement Alternat Med. 2006 Sep;3(3):349-57.
- Mohandas Rao K G, Muddanna Rao, Gurumadhva Rao. Enhancement of hippocampal CA3 neuronal dendritic arborization by Centella asiatica (Linn) fresh leaf extract treatment in adult Rats. Journal of the Chinese Medical Association. 2008;71(1):6-13.
- Mizoguchi K, Kunishita T, Chui DH, Tabira T. Stress induces neuronal death in the hippocampus of castrated rats. Neurosci Lett 1992;138:157-60.
- Stein-Behrens BA, Lin WJ, Sapolsky RM. Physiological elevations of glucocorticoids potentiate glutamate accumulation in the hippocampus. J Neurochem 1994a;63(2):596-602
- Gilad GM, Gilad VH, Wyatt JR, Tizobers Y. Region selective stress induced increase of glutamate uptake and release in rat forebrain. Brain Res. 1990;525:335-338.
- Steward O. Topographic organization of the projections from the entorhinal area to the hippocampal formation of the rat. J Comp Neurol.1976;167(3):285-314.
- Crawford IL, Connor JD. Localization and release of glutamic acid in relation to the hippocampal mossy fibre pathway. Nature.1973;244(5416):442-3
- Sunanda, Meti BL, Raju TR. Entorhinal cortex lesioning protects hippocampal CA3 neurons from stress-induced damage. Brain Res; 1997;770(1-2):302-6.
- McEwen BS. Stress and hippocampal plasticity. Annu Rev Neurosci. 1999;22:105-22.
- McEwen BS, Sapolsky RM. Stress and cognitive function. Curr Opin Neurobiol. 1995;5(2):205-16.
- Magarinos AM, McEwen BS. Stress induced atrophy of apical dendrites of hippocampal CA3 neurons: involvement of glucocorticoid secretion and excitatory amino acid receptors. Neurosci. 1995a:69: 89-98.
- Moghaddam B. Stress preferentially increases extraneuronal levels of excitatory amino acids in the prefrontal cortex: comparison to hippocampus and basal ganglia. J Neurochem. 1993;60(5):1650-7.
- Moghaddam B, Bolinao ML, Stein-Behrens B, Sapolsky R. Glucocorticoids mediate the stress-induced extracellular accumulation of glutamate. Brain Res 1994;655(1-2):251-4.
- Chou YC, Lin WJ, Sapolsky RM. Glucocorticoids increase extracellular [3H]D-aspartate overflow in hippocampal cultures during cyanide-induced ischemia. Brain Res. 1994;654(1):8-14.
- Hiramine C. [Definition and morphological features of apoptosis]. Rinsho Byori. 1997;45(5):459-69.
- Sloviter RS, Dean E, Sollas AL, Goodman JH. Apoptosis and necrosis induced in different hippocampal neuron populations by repetitive perforant path stimulation in the rat. J Comp Neurol. 1996 Mar 11;366(3):516-33.
- McAllister AK, Katz LC, Lo DC. Neurotrophins and synaptic plasticity. Annu Rev Neurosci. 1999;22:295-318.
- Smith MA, Makino S, Kvetnansky R, Post RM. Stress and glucocorticoids affect the expression of brain-derived neurotrophic factor and neurotrophin-3 mRNAs in the hippocampus. J Neurosci. 1995;15(3 Pt 1):1768-77.
- Nitta A, Ohmiya M, Sometani A, Itoh M, Nomoto H, Furukawa Y, Furukawa S. Brain-derived neurotrophic factor prevents neuronal cell death induced by corticosterone. J Neurosci Res 1999;57(2):227-35.
- Kempermann G, Kuhn HG, Gage FH . Experience-induced neurogenesis in the senescent dentate gyrus. J Neurosci 1998;18(9):3206-12.
- Gould E, Tanapat P. Stress and hippocampal neurogenesis. Biol Psychiatry. 1999 Dec 1;46(11):1472-9.
- Lemaire V, Aurousseau C, Le Moal M, Abrous DN. Behavioural trait of reactivity to novelty is related to hippocampal neurogenesis. Eur J Neurosci.1999;11(11):4006-14.
- Gould E, Tanapat P, Rydel T, Hastings N. Regulation of hippocampal neurogenesis in adulthood. Biol Psychiatry. 2000 Oct 15;48(8):715-20.
- Ramanathan M, Sivakumar S, Anandvijayakumar PR, Saravanababu C, Pandian PR. Neuroprotective evaluation of standardized extract of Centella asciatica in monosodium glutamate treated rats. Indian J Exp Biol. 2007;45(5):425-31.
- Gnanapragasam A, Ebenezar KK, Sathish V, Govindaraju P, Devaki T. Protective effect of Centella asiatica on antioxidant tissue defense system against adriamycin induced cardiomyopathy in rats. Life Sci. 2004;76(5):585-97.
- Lee MK, Kim SR, Sung SH, Lim D, Kim H, Choi H, Park HK, Je S, Ki YC. Asiatic acid derivatives protect cultured cortical neurons from glutamate-induced excitotoxicity. Res Commun Mol Pathol Pharmacol.2000;108(1-2):75-86.
Hemamalini, Rao M.S. EFFECT OF CENTELLA ASIATICA LEAVES EXTRACT ON HIPPOCAMPAL CA3 NEURONS: A MORPHOLOGICAL STUDY IN MICE. Int J Anat Res 2016;4(1):1961-1966. DOI: 10.16965/ijar.2016.121