{"id":626,"date":"2017-06-30T03:24:55","date_gmt":"2017-06-30T03:24:55","guid":{"rendered":"http:\/\/www.ijmhr.org\/IntJAnatRes\/?page_id=626"},"modified":"2017-06-30T03:25:54","modified_gmt":"2017-06-30T03:25:54","slug":"ijar-2017-218","status":"publish","type":"page","link":"https:\/\/www.ijmhr.org\/IntJAnatRes\/ijar-2017-218","title":{"rendered":"IJAR.2017.218"},"content":{"rendered":"
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Type of Article:<\/strong>\u00a0\u00a0Original Research<\/h3>\n

Volume 5; Issue 2.3 (June 2017)<\/strong><\/h3>\n

Page No.:<\/strong> 3913-3922<\/h3>\n

DOI:\u00a0<\/strong>https:\/\/dx.doi.org\/10.16965\/ijar.2017.218<\/h3>\n

CORRELATING SPERM REACTIVE OXYGEN SPECIES PRODUCTION AND ITS MORPHOLOGICAL DEFECTS \u2013 WHICH CAN BE THE BEST POSSIBLE MORPHOLOGICAL PREDICTOR OF OXIDATIVE DAMAGE IN ROUTINE SCREENING?<\/h3>\n

Dinesh Kumar. V *1<\/sup>, Swetasmita Mishra 2<\/sup>, Rima Dada 2<\/sup>,\u00a0 Shaik Hussain Saheb 3<\/sup>.<\/strong><\/p>\n

*1 <\/sup>Assistant Professor, Department of Anatomy, Pondicherry Institute of Medical Sciences, Puducherry, India.<\/p>\n

2<\/sup> Laboratory of molecular reproduction and genetics, All India Institute of Medical Sciences, New Delhi, India.<\/p>\n

3<\/sup> Assistant professor, Department of Anatomy, JJM Medical College, Davangere, Karnataka. India.<\/p>\n

Address for Correspondence: <\/strong>Dr. Dinesh Kumar. V, Assistant Professor, Department of Anatomy, Pondicherry Institute of Medical Sciences, Puducherry, India. E-Mail: <\/strong>dinesh.88560@gmail.com<\/p>\n

ABSTRACT<\/strong><\/p>\n

Introduction: <\/strong>Male infertility contributes to nearly 30% of the total infertile population worldwide. Despite the advances in diagnostic workup of an infertile male in majority (40-50%) of the cases aetiology is not defined. Supra-physiological levels of ROS impair sperm function by damaging polyunsaturated fatty acid-rich sperm membrane and mitochondrial and nuclear DNA.<\/p>\n

Materials and Methods: <\/strong>In this study, we performed Chemiluminescence based estimation of Reactive Oxygen Species levels (ROS) in 40 men with idiopathic infertility and 40 age-matched fertile men who served as controls. We had correlated the ROS levels with morphological defects to find out the single best predictor for oxidative stress in sperm.<\/p>\n

Results and Discussion: <\/strong>In 74.48% infertile men, seminal ROS levels were higher than the critical value (\u2264 22 RLU\/sec\/million). A significant (p<0.05) difference was observed in the number of head defects, mid-piece defects, cytoplasmic droplets and sperm deformity index (SDI) between cases and controls.<\/p>\n

Conclusion: <\/strong>Supra-physiological ROS levels can impair the sperm function, thus resulting in infertility and excessive ROS generation is mostly by the morphologically abnormal spermatozoa. SDI had significant positive correlation with the ROS levels both in cases (p=0.00) and controls (p=0.001) suggesting it as the best surrogate marker for ROS mediated sperm damage.<\/p>\n

KEY WORDS:<\/strong> Sperm Reactive Oxygen Species, Male Infertility, Morphological Defects, Oxidative Damage, Chemiluminescence.<\/p>\n

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    \n \t Cite this article:<\/strong><\/span> Dinesh Kumar. V, Swetasmita Mishra, Rima Dada, Shaik Hussain Saheb. CORRELATING SPERM REACTIVE OXYGEN SPECIES PRODUCTION AND ITS MORPHOLOGICAL DEFECTS \u2013 WHICH CAN BE THE BEST POSSIBLE MORPHOLOGICAL PREDICTOR OF OXIDATIVE DAMAGE IN ROUTINE SCREENING?. Int J Anat Res 2017;5(2.3):3913-3922. DOI:\u00a0<\/strong>10.16965\/ijar.2017.218\u00a0 \n <\/div>\t\n\t\t\n","protected":false},"excerpt":{"rendered":"

    Type of Article:\u00a0\u00a0Original Research Volume 5; Issue 2.3 (June 2017) Page No.: 3913-3922 DOI:\u00a0https:\/\/dx.doi.org\/10.16965\/ijar.2017.218 CORRELATING SPERM REACTIVE OXYGEN SPECIES PRODUCTION AND ITS MORPHOLOGICAL DEFECTS \u2013 WHICH CAN BE THE BEST POSSIBLE MORPHOLOGICAL PREDICTOR OF OXIDATIVE DAMAGE IN ROUTINE SCREENING? Dinesh Kumar. V *1, Swetasmita Mishra 2, Rima Dada 2,\u00a0 Shaik Hussain Saheb 3. *1 Assistant
    + Read More<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/626"}],"collection":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/comments?post=626"}],"version-history":[{"count":1,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/626\/revisions"}],"predecessor-version":[{"id":629,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/626\/revisions\/629"}],"wp:attachment":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/media?parent=626"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}