{"id":2946,"date":"2024-05-30T09:22:57","date_gmt":"2024-05-30T09:22:57","guid":{"rendered":"https:\/\/www.ijmhr.org\/IntJAnatRes\/?page_id=2946"},"modified":"2024-05-30T09:43:26","modified_gmt":"2024-05-30T09:43:26","slug":"ijar-2024-104","status":"publish","type":"page","link":"https:\/\/www.ijmhr.org\/IntJAnatRes\/ijar-2024-104","title":{"rendered":"IJAR.2024.104"},"content":{"rendered":"<div class=\"su-row\"><div class=\"su-column su-column-size-1-2\"><div class=\"su-column-inner su-u-clearfix su-u-trim\"><div class=\"su-button-center\"><a href=\"https:\/\/www.ijmhr.org\/ijar.12.2\/IJAR.2024.104.pdf\" class=\"su-button su-button-style-default\" style=\"color:#FFFFFF;background-color:#6b0e00;border-color:#560c00;border-radius:5px;-moz-border-radius:5px;-webkit-border-radius:5px\" target=\"_self\"><span style=\"color:#FFFFFF;padding:6px 16px;font-size:13px;line-height:20px;border-color:#98574d;border-radius:5px;-moz-border-radius:5px;-webkit-border-radius:5px;text-shadow:none;-moz-text-shadow:none;-webkit-text-shadow:none\"><i class=\"sui sui-cloud-download\" style=\"font-size:13px;color:#FFFFFF\"><\/i> DOWNLOAD PDF<\/span><\/a><\/div><\/div><\/div> <div class=\"su-column su-column-size-1-2\"><div class=\"su-column-inner su-u-clearfix su-u-trim\"><div class=\"su-button-center\"><a href=\"https:\/\/ijmhr.org\/ijar-vol-12-2.htm\" class=\"su-button su-button-style-default\" style=\"color:#FFFFFF;background-color:#6b0e00;border-color:#560c00;border-radius:5px;-moz-border-radius:5px;-webkit-border-radius:5px\" target=\"_self\"><span style=\"color:#FFFFFF;padding:6px 16px;font-size:13px;line-height:20px;border-color:#98574d;border-radius:5px;-moz-border-radius:5px;-webkit-border-radius:5px;text-shadow:none;-moz-text-shadow:none;-webkit-text-shadow:none\"><i class=\"sui sui-book\" style=\"font-size:13px;color:#FFFFFF\"><\/i> Table of Contents<\/span><\/a><\/div><\/div><\/div><\/div>\n<h3 style=\"text-align: justify;\"><strong>Type of Article:<\/strong> \u00a0Original Research<\/h3>\n<h3 style=\"text-align: justify;\"><strong>Volume 12; Issue 2 (June 2024)<\/strong><\/h3>\n<h3 style=\"text-align: justify;\"><strong>Page No.:<\/strong> 8903-8909<\/h3>\n<h3 style=\"text-align: justify;\"><strong>DOI:\u00a0<\/strong>https:\/\/dx.doi.org\/10.16965\/ijar.2024.104<\/h3>\n<h3 style=\"text-align: justify;\">Tissue Processing using Microwave Oven: A Boon for Histology Slide Preparation<\/h3>\n<p><strong>Dane Chandy <sup>1<\/sup>, Prima Swetha D\u2019Souza <sup>*2<\/sup>, Susie Jeyalyn David <sup>3<\/sup>.<\/strong><\/p>\n<p style=\"text-align: justify;\"><sup>1 <\/sup>Assistant Professor, Department of Anatomy, Father Muller Medical College, Mangalore, Karnataka, India. <strong>ORCiD: <\/strong>0000-0003-0800-8894<\/p>\n<p style=\"text-align: justify;\"><sup>*2 <\/sup>Assistant Professor, Department of Anatomy, K. S. Hegde Medical Academy, Mangalore<strong>, <\/strong>Karnataka, India. <strong>ORCiD: <\/strong>0009-0007-3355-2601<\/p>\n<p style=\"text-align: justify;\"><sup>3 <\/sup>Assistant Professor, Department of Anatomy, Sree Mookambika Institute of Medical Sciences, Kulasekharam, Kanyakumari District, Tamilnadu, India.\u00a0 <strong>ORCiD:<\/strong>\u00a00000- 0001-6509-8879<\/p>\n<p style=\"text-align: justify;\"><strong>Corresponding Author:<\/strong> Dr. Prima Swetha D\u2019Souza, Assistant Professor, Department of Anatomy, K. S. Hegde Medical Academy, Mangalore<strong>, <\/strong>Karnataka, India.\u00a0<strong>E-Mail:<\/strong> <a href=\"mailto:prima@nitte.edu.in\">prima@nitte.edu.in<\/a><\/p>\n<p style=\"text-align: justify;\"><strong>ABSTRACT<\/strong>\u00a0<\/p>\n<p style=\"text-align: justify;\"><strong>Background:<\/strong> Tissue processing is an important step in histology laboratories. Routine tissue processing is time-consuming. Due to the same time factor, microwave tissue processing is now increasingly being used in many histology and pathology laboratories.<\/p>\n<p style=\"text-align: justify;\"><strong>Objective:<\/strong> To compare the time taken to process the tissues using the routine conventional and microwave methods.<\/p>\n<p style=\"text-align: justify;\"><strong>Methods:<\/strong> The study was conducted in the Histology laboratory. 100 slides (50 conventional method + 50 microwave method) were processed using both methods, sectioned, stained, and analyzed by an independent observer who was unaware of the processing method.<\/p>\n<p style=\"text-align: justify;\"><strong>Results: <\/strong>The study showed a significant decrease in processing time by the microwave method (p-value &lt; .0001).<\/p>\n<p style=\"text-align: justify;\"><strong>Conclusion: <\/strong>The present study concluded that the time taken for dehydrating, clearing, and embedding the tissues in paraffin wax was found to be considerably lower than that taken for the conventional method of tissue processing. Chloroform was used here as a clearing agent, and it had desirable effects.<\/p>\n<p><strong>Keywords<\/strong><strong>:<\/strong> Microwave tissue processing, Conventional tissue processing.<\/p>\n<p style=\"text-align: justify;\"><strong>REFERENCES<\/strong><\/p>\n<p style=\"text-align: justify;\">[1]. Shoja MM, Tubbs RS, Loukas M, Shokouhi G, Ardalan MR. Marie-Fran\u00e7ois Xavier Bichat (1771-1802) and his contributions to the foundations of pathological anatomy and modern medicine. Ann Anat. 2008 Nov 20;190(5):413-20.<br \/>https:\/\/doi.org\/10.1016\/j.aanat.2008.07.004<br \/>PMid:18819786<br \/>[2]. Visinoni F. Ultra rapid microwave\/variable pressure induced histoprocessing: description of a new tissue processor. J Histotechnol. 1998;21(3):219-24.<br \/>https:\/\/doi.org\/10.1179\/his.1998.21.3.219<br \/>[3]. Shruthi BS, Vinodhkumar P, Kashyap B, Reddy PS. Use of microwave in diagnostic pathology. J Can Res Ther. 2013 Oct;9(3):351-55.<br \/>https:\/\/doi.org\/10.4103\/0973-1482.119301<br \/>PMid:24125965<br \/>[4]. Klump VR. Working Procedures in Microwave Histology [Internet]. Available from:http:\/\/diyhpl.us\/~bryan\/irc\/protocol-online\/protocol-cache\/microwave.html<br \/>[5]. Sivadas P, Kumar H, Lakashmanan C, Bhardwaj JR. Microwave stimulated fixation and rapid processing of tissue for histopathology. Med J Armed Forces India. 1996 Jul; 52(3):157-60.<br \/>https:\/\/doi.org\/10.1016\/S0377-1237(17)30791-8<br \/>PMid:28769375<br \/>[6]. Kok LP, Boon ME. Microwaves for microscopy. J Microsc. 1990 Jun;158(3):291-322.<br \/>https:\/\/doi.org\/10.1111\/j.1365-2818.1990.tb03003.x<br \/>PMid:2203910<br \/>[7]. Kok LP, Boon ME. Physics of microwave technology in histochemistry. Histochem J. 1990; 22:381-8.<br \/>https:\/\/doi.org\/10.1007\/BF01003174<br \/>PMid:2211184<br \/>[8]. Datta A. Principles of General Anatomy. Kolkata: KP Basu Publishing Co.; 2001.<br \/>[9]. Bennett HS. The role of histology in medical education and biological thinking. Anat Rec. 1956 Jun;125(2):327-54.<br \/>https:\/\/doi.org\/10.1002\/ar.1091250210<br \/>PMid:13354992<br \/>[10]. Hussein IH, Raad M, Safa R, Jurjus R, Jurjus A. Once Upon a Microscopic Slide: The Story of Histology. Journal of Cytology &amp; Histology. 2015;06(06):1-4.<br \/>https:\/\/doi.org\/10.4172\/2157-7099.1000377<br \/>[11]. Bosch F, Rosich L. The contributions of Paul Ehrlich to pharmacology: a tribute on the occasion of the centenary of his Nobel Prize. Pharmacology. 2008;82(3):171-79.<br \/>https:\/\/doi.org\/10.1159\/000149583<br \/>PMid:18679046 PMCid:PMC2790789<br \/>[12]. Chen X, Zheng B, Liu H. Optical and digital microscopic imaging techniques and applications in pathology. Anal Cell Pathol (Amst). 2011;34(1-2):5-18.<br \/>https:\/\/doi.org\/10.1155\/2011\/150563<br \/>[13]. Kango PG, Deshmukh RS. Microwave processing: A boon for oral pathologists. J Oral Maxillofac Pathol. 2011 Mar;5(1):6-13.<br \/>https:\/\/doi.org\/10.4103\/0973-029X.80031<br \/>PMid:21731271 PMCid:PMC3125658<br \/>[14]. Kumar H, Kalkal P, Chandanwale SS, Bamanikar S, Jain A. Role of microwaves in rapid processing of tissues for histopathology. Med J DY Patil Univ. 2014 Jun;7(4):45862.<br \/>https:\/\/doi.org\/10.4103\/0975-2870.135267<br \/>[15]. Morales AR, Essenfeld H, Essenfeld E, Duboue MC, Vincek V, Nadji M. Continuous-specimen-flow, high-throughput, 1-hour tissue processing. A system for rapid diagnostic tissue preparation. Arch Pathol Lab Med. 2002 May;126(5):583-90.<br \/>https:\/\/doi.org\/10.5858\/2002-126-0583-CSFHTH<br \/>PMid:11958665<br \/>[16]. Leong ASY. Microwaves and turnaround times in histoprocessing: Is this a new era in histotechnology? Am J Clin Pathol. 2004 Apr;121(4):46062.<br \/>https:\/\/doi.org\/10.1309\/PLQ523DENNH8R00Q<br \/>PMid:15080296<\/p>\n<p style=\"text-align: justify;\">\n\t\t\t <div class=\"promo1\" style=\"background-color:#f7f7f7; border-color: #6b0e00 #e8e6e6 #e8e6e6;\">\n             \t <span style=\"color: #800000;\"><strong>Cite this article:<\/strong><\/span> Dane Chandy, Prima Swetha D\u2019Souza, Susie Jeyalyn David. Tissue Processing using Microwave Oven: A Boon for Histology Slide Preparation. Int J Anat Res 2024;12(2):8903-8909. <strong>DOI:\u00a0<\/strong>10.16965\/ijar.2024.104\u00a0 \n             <\/div>\t\n\t\t\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Type of Article: \u00a0Original Research Volume 12; Issue 2 (June 2024) Page No.: 8903-8909 DOI:\u00a0https:\/\/dx.doi.org\/10.16965\/ijar.2024.104 Tissue Processing using Microwave Oven: A Boon for Histology Slide Preparation Dane Chandy 1, Prima Swetha D\u2019Souza *2, Susie Jeyalyn David 3. 1 Assistant Professor, Department of Anatomy, Father Muller Medical College, Mangalore, Karnataka, India. ORCiD: 0000-0003-0800-8894 *2 Assistant Professor,<br \/><a class=\"moretag\" href=\"https:\/\/www.ijmhr.org\/IntJAnatRes\/ijar-2024-104\">+ Read More<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/2946"}],"collection":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/comments?post=2946"}],"version-history":[{"count":2,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/2946\/revisions"}],"predecessor-version":[{"id":2955,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/2946\/revisions\/2955"}],"wp:attachment":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/media?parent=2946"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}