{"id":2943,"date":"2024-05-30T09:57:01","date_gmt":"2024-05-30T09:57:01","guid":{"rendered":"https:\/\/www.ijmhr.org\/IntJAnatRes\/?page_id=2943"},"modified":"2024-05-30T09:57:01","modified_gmt":"2024-05-30T09:57:01","slug":"ijar-2024-117","status":"publish","type":"page","link":"https:\/\/www.ijmhr.org\/IntJAnatRes\/ijar-2024-117","title":{"rendered":"IJAR.2024.117"},"content":{"rendered":"<div class=\"su-row\"><div class=\"su-column su-column-size-1-2\"><div class=\"su-column-inner su-u-clearfix su-u-trim\"><div class=\"su-button-center\"><a href=\"https:\/\/www.ijmhr.org\/ijar.12.2\/IJAR.2024.117.pdf\" class=\"su-button su-button-style-default\" style=\"color:#FFFFFF;background-color:#6b0e00;border-color:#560c00;border-radius:5px;-moz-border-radius:5px;-webkit-border-radius:5px\" target=\"_self\"><span style=\"color:#FFFFFF;padding:6px 16px;font-size:13px;line-height:20px;border-color:#98574d;border-radius:5px;-moz-border-radius:5px;-webkit-border-radius:5px;text-shadow:none;-moz-text-shadow:none;-webkit-text-shadow:none\"><i class=\"sui sui-cloud-download\" style=\"font-size:13px;color:#FFFFFF\"><\/i> DOWNLOAD PDF<\/span><\/a><\/div><\/div><\/div> <div class=\"su-column su-column-size-1-2\"><div class=\"su-column-inner su-u-clearfix su-u-trim\"><div class=\"su-button-center\"><a href=\"https:\/\/ijmhr.org\/ijar-vol-12-2.htm\" class=\"su-button su-button-style-default\" style=\"color:#FFFFFF;background-color:#6b0e00;border-color:#560c00;border-radius:5px;-moz-border-radius:5px;-webkit-border-radius:5px\" target=\"_self\"><span style=\"color:#FFFFFF;padding:6px 16px;font-size:13px;line-height:20px;border-color:#98574d;border-radius:5px;-moz-border-radius:5px;-webkit-border-radius:5px;text-shadow:none;-moz-text-shadow:none;-webkit-text-shadow:none\"><i class=\"sui sui-book\" style=\"font-size:13px;color:#FFFFFF\"><\/i> Table of Contents<\/span><\/a><\/div><\/div><\/div><\/div>\n<h3 style=\"text-align: justify;\"><strong>Type of Article:<\/strong> \u00a0Original Research<\/h3>\n<h3 style=\"text-align: justify;\"><strong>Volume 12; Issue 2 (June 2024)<\/strong><\/h3>\n<h3 style=\"text-align: justify;\"><strong>Page No.:<\/strong> 8924-8930<\/h3>\n<h3 style=\"text-align: justify;\"><strong>DOI:\u00a0<\/strong>https:\/\/dx.doi.org\/10.16965\/ijar.2024.117<\/h3>\n<h3 style=\"text-align: justify;\">Temporomandibular Joint Histology<\/h3>\n<p><strong>Daniela Botero-Gonz\u00e1lez .<\/strong><\/p>\n<p><em><sup>*1 <\/sup><\/em>Assistant professor, Department of Basic Medical Sciences, Faculty of Health Science, Universidad Tecnol\u00f3gica de Pereira, Pereira, Colombia. <a href=\"https:\/\/orcid.org\/0000-0002-0156-4997\"><strong>ORCiD<\/strong> 0000-0002-0156-4997<\/a><\/p>\n<p><strong>Corresponding Author:<\/strong> Daniela Botero-Gonz\u00e1lez, OD, MSc, PhD., Assistant professor, Department of Basic Medical Sciences, Faculty of Health Science, Universidad Tecnol\u00f3gica de Pereira, Pereira, Colombia. <strong>E-Mail:<\/strong> dbotero@utp.edu.co.<\/p>\n<p style=\"text-align: justify;\"><strong>ABSTRACT<\/strong>\u00a0<\/p>\n<p style=\"text-align: justify;\"><strong>Background:<\/strong> The temporomandibular joint plays a crucial role in daily oral functions like mastication. A comprehensive understanding of the histological components of these joints proves valuable in deciphering their intricate anatomical structures.<\/p>\n<p style=\"text-align: justify;\"><strong>Purpose of the study:<\/strong> This study aimed to compare the histochemical stains of hematoxylin-eosin and Goldner&#8217;s trichrome applied to a temporomandibular joint sample using the Wistar Rat as a biomodel.<\/p>\n<p style=\"text-align: justify;\"><strong>Results:<\/strong> Complete sagittal slices of murine biomodel hemicranium were\u00a0evaluated and the comprehensive histological technique involved various steps, including perfusion, post-fixation, decalcification, dehydration, clarification, inclusion, cutting, staining, and assembly. The temporomandibular joint specimens were stained using H&amp;E and Goldner&#8217;s trichrome. The histological description included structures such as the external auditory canal, mandibular fossa and condyle, pterygoid muscles, joint capsule, synovial membrane, articular disc, and articular surface. Special cell types like chondrocytes and synovial cells were also identified.<\/p>\n<p style=\"text-align: justify;\"><strong>Conclusion:<\/strong> Goldner&#8217;s trichrome, an underutilized technique, exhibited superior chromatic richness in differentiating the cellular and tissue components of the temporomandibular joint.<\/p>\n<p><strong>Keywords<\/strong><strong>:<\/strong> Histology, Joint Capsule, Synovial Membrane, Temporomandibular Joint, Temporomandibular Joint Disc, Trichrome Stain, Wistar Rats.<\/p>\n<p style=\"text-align: justify;\"><strong>REFERENCES<\/strong><\/p>\n<p style=\"text-align: justify;\">[1]. Suvarna SK, Bancroft JD, Layton D. Bancoft&#8217;s theory and practice of histological techniques. 8th ed. Massachusetts: Elsevier; 2019. https:\/\/doi.org\/10.1016\/C2015-0-00143-5<br \/>[2]. Chiego DJ. Principios de histolog\u00eda y embriolog\u00eda bucal con orientaci\u00f3n cl\u00ednica. 4th ed. Barcelona: Elsevier; 2014.<br \/>[3]. G\u00f3mez de Ferraris ME, Campos Mu\u00f1oz A. Histolog\u00eda, embriolog\u00eda e ingenier\u00eda tisular bucodental. 4th ed. M\u00e9xico: Editorial m\u00e9dica panamericana; 2019.<br \/>[4]. Garant P. Oral cells and tissues. 1st ed. Italia: Quintessence Publishing; 2003.<br \/>[5]. Liang W, Li X, Gao B, Gan H, Lin X, Liao L, et al. Observing the development of the temporomandibular joint in embryonic and post-natal mice using various staining methods. Exp Ther Med 2016; 11(2):481-9.<br \/>https:\/\/doi.org\/10.3892\/etm.2015.2937<br \/>PMid:26893634 PMCid:PMC4734204<br \/>[6]. Goldner J. A Modification of the Masson Trichrome Technique for Routine Laboratory Purposes. Am J Pathol 1937; 14(2):237-243.<br \/>[7]. Schmitz N, Laverty S, Kraus V, Aigner T. Basic methods in histopathology of joint tissues. Osteoarthritis and Cartilage 2010; 18:S113-S6.<br \/>https:\/\/doi.org\/10.1016\/j.joca.2010.05.026<br \/>PMid:20864017<br \/>[8]. Garc\u00eda R. Laboratorio de anatom\u00eda patol\u00f3gica. 1st ed. Madrid: Interamericana-McGraw-Hill; 1993.<br \/>[9]. Gage GJ, Kipke DR, Shain W. Whole Animal Perfusion Fixation for Rodents. JoVE 2012; 65:3564.<br \/>https:\/\/doi.org\/10.3791\/3564-v<br \/>PMid:22871843 PMCid:PMC3476408<br \/>[10]. Gruber H. Adaptation of Goldner&#8217;s Masson trichrome stain for the study of undecalcified plastic embedded bone. Biotech Histochem 1992; 67(1):30-34.<br \/>https:\/\/doi.org\/10.3109\/10520299209110002<br \/>PMid:1617000<br \/>[11]. Orset E, Chaffanjon P, Bettega G. Temporomandibular joint model : anatomic and radiologic comparison between rat and human. Surg Radiol Anat 2014; 36(2):163-166.<br \/>https:\/\/doi.org\/10.1007\/s00276-013-1159-4<br \/>PMid:23811934<br \/>[12]. Porto GG, Vasconcelos BC, Andrade ES, Silva-Junior VA. Comparison between human and rat TMJ: anatomic and histopathologic features. Acta Cir\u00fargica Brasileira 2010; 25(3):290-293.<br \/>https:\/\/doi.org\/10.1590\/S0102-86502010000300012<br \/>PMid:20498943<br \/>[13]. Kato T, Takahashi S, Domon T. Effects of a Liquid Diet on the Temporomandibular Joint of Growing Rats. Med Princ Pract 2015; 24(3):257-262.<br \/>https:\/\/doi.org\/10.1159\/000370072<br \/>PMid:25614129 PMCid:PMC5588221<br \/>[14]. Yang B, Tian C, Zhang ZG, Han FC, Azem R, Yu H, et al. Sh3pxd2b mice are a model for craniofacial dysmorphology and otitis media. PLoS One 2011; 6(7):e22622.<br \/>https:\/\/doi.org\/10.1371\/journal.pone.0022622<br \/>PMid:21818352 PMCid:PMC3144925<br \/>[15]. Cavalcanti UDNT, Baratella L, Neto JE, Castro RM, Cardona AS, Melo MLM, et al. Morphological aspects of the embryonic development of the TMJ in rats (Rattus norvegicus albinus) treated with fluoxetine. Int J Morphol 2009; 27(3): 899-903.<br \/>https:\/\/doi.org\/10.4067\/S0717-95022009000300041<br \/>[16]. Luna LG. Histopathological Methods and Color Atlas of Special Stain and Tissue Artifacts. 1st ed. Gaithersburg: Downers Grove; 1992.<br \/>[17]. Ramfjord A, Ash M. Occlusion. 2th ed. Filadelfia: Interamericana; 1972.<br \/>[18]. Rubiano C. Tratamiento con placas y correcci\u00f3n oclusal por tallado selectivo. 1st ed. Bogot\u00e1: Amolca; 2005.<br \/>[19]. Latarjet M, Ruiz-Liard A. Anatom\u00eda Humana. 2th ed. Buenos Aires: Editorial M\u00e9dica Panamericana; 2005.<br \/>[20]. Fuentes R, Cant\u00edn M, Ottone NE, Bucchi C. Caracterizaci\u00f3n de los componentes \u00f3seos de la articulaci\u00f3n temporomandibular: Una revisi\u00f3n de la literatura. Int J Morphol 2015; 33(4):1569-1576.<br \/>https:\/\/doi.org\/10.4067\/S0717-95022015000400062<br \/>[21]. Yamaki Y, Tsuchikawa K, Nagasawa T, Hiroyasu K. Embryological study of the development of the rat temporomandibular joint : highlighting the development of the glenoid fossa. Odontology 2005; 93:30-34.<br \/>https:\/\/doi.org\/10.1007\/s10266-005-0046-9<br \/>PMid:16170473<br \/>[22]. Basting RT, Napimoga MH, De Lima JM, De Freitas NS, Clemente-Napimoga JT. Fast and accurate protocol for histology and immunohistochemistry reactions in temporomandibular joint of rats. Archives of Oral Biology 2021; 126:105115.<br \/>https:\/\/doi.org\/10.1016\/j.archoralbio.2021.105115<br \/>PMid:33819835<br \/>[23]. Sanderson C. Entering the Realm of Mineralized Bone Processing: A Review of the Literature and Techniques. Journal of Histotechnology, 1997; 20(3):259-266.<br \/>https:\/\/doi.org\/10.1080\/01478885.1997.11878769<br \/>[24]. Farf\u00e1n C, Quidel B, Borie-echevarr\u00eda E, Fuentes R. Anatomical and histological components of the bilaminar zone of the temporomandibular joint. A narrative review. Int. J. Morphol., 2021; 39(2):477-483.<br \/>https:\/\/doi.org\/10.4067\/S0717-95022021000200477<\/p>\n<p style=\"text-align: justify;\">\n\t\t\t <div class=\"promo1\" style=\"background-color:#f7f7f7; border-color: #6b0e00 #e8e6e6 #e8e6e6;\">\n             \t <span style=\"color: #800000;\"><strong>Cite this article:<\/strong><\/span> Daniela Botero-Gonz\u00e1lez. Temporomandibular Joint Histology. Int J Anat Res 2024;12(2):8924-8930. <strong>DOI:\u00a0<\/strong>10.16965\/ijar.2024.117\u00a0 \n             <\/div>\t\n\t\t\n\n\n<p><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Type of Article: \u00a0Original Research Volume 12; Issue 2 (June 2024) Page No.: 8924-8930 DOI:\u00a0https:\/\/dx.doi.org\/10.16965\/ijar.2024.117 Temporomandibular Joint Histology Daniela Botero-Gonz\u00e1lez . *1 Assistant professor, Department of Basic Medical Sciences, Faculty of Health Science, Universidad Tecnol\u00f3gica de Pereira, Pereira, Colombia. ORCiD 0000-0002-0156-4997 Corresponding Author: Daniela Botero-Gonz\u00e1lez, OD, MSc, PhD., Assistant professor, Department of Basic Medical Sciences,<br \/><a class=\"moretag\" href=\"https:\/\/www.ijmhr.org\/IntJAnatRes\/ijar-2024-117\">+ Read More<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/2943"}],"collection":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/comments?post=2943"}],"version-history":[{"count":1,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/2943\/revisions"}],"predecessor-version":[{"id":2960,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/2943\/revisions\/2960"}],"wp:attachment":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/media?parent=2943"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}