{"id":2337,"date":"2020-08-11T15:48:54","date_gmt":"2020-08-11T15:48:54","guid":{"rendered":"http:\/\/www.ijmhr.org\/IntJAnatRes\/?page_id=2337"},"modified":"2020-08-11T15:48:54","modified_gmt":"2020-08-11T15:48:54","slug":"ijar-2020-190","status":"publish","type":"page","link":"https:\/\/www.ijmhr.org\/IntJAnatRes\/ijar-2020-190","title":{"rendered":"IJAR.2020.190"},"content":{"rendered":"
<\/i> DOWNLOAD PDF<\/span><\/a><\/div><\/div><\/div>
<\/i> Table of Contents<\/span><\/a><\/div><\/div><\/div><\/div>\n

Type of Article:<\/strong>\u00a0\u00a0Original Research<\/h3>\n

Volume 8; Issue 3.2 (August 2020)<\/strong><\/h3>\n

Page No.:<\/strong>\u00a07703-7706<\/h3>\n

DOI:\u00a0<\/strong>https:\/\/dx.doi.org\/10.16965\/ijar.2020.190<\/h3>\n

EFFECT OF VARIABLE DOSES OF CISPLATIN ON MICROANATOMY OF TESTIS IN ALBINO RATS<\/h3>\n

Rabiya Amin 1<\/sup>, \u00a0Ubaid Ali *2<\/sup>, Maheen Nazir 3<\/sup>.<\/strong><\/p>\n

1<\/sup> Senior resident, Department of Anatomy, Dr Chandramma Dayananda Sagar Institute Of Medical Education\u00a0 And Research, Harohalli, Bangalore, Karnataka, India.<\/p>\n

*2 <\/sup>Department of Urology, Institute of Nephrourology, Bangalore, Karnataka, India.<\/p>\n

3 <\/sup>Senior resident, Department of Anatomy, Govt medical college Srinagar, India.<\/p>\n

Corresponding\u00a0 Author: <\/strong>Dr Ubaid Ali, Department of urology .Institute of Nephrourology Bangalore, Karnataka, India. Phone number -7006560089 E-Mail:<\/strong> dr.ubaidali22@gmail.com<\/p>\n

ABSTRACT<\/h3>\n

Cisplatin is an organic metal co-ordination complex and is\u00a0 a widely used chemotherapeutic agent against a variety of malignant tumors, \u00a0like sarcomas and carcinomas. It interferes with the DNA synthesis and causes apoptosis in view of the fact that the growth of normal cells is also affected. These toxic effects alter the metabolic function of certain tissues and organs. .60 albino rats weighing on an average 100 grams were taken. The animals were divided into three groups. Group A animals received no drug. Group B was injected with 1.3mg\/m2 <\/sup>of cisplatin by intraperitoneal route and group C received \u00a02.5mg\/m2<\/sup>of cisplatin. The process of drug administration was continued for 12 weeks. The animals were sacrificed in five sittings, after interval of 1,3,6,9 and 12 weeks after drug administration. At the termination of experiments the testis were fixed and stained with H&E. There was degeneration of germ cells with increase in capsular wall thickness of testis, and leydig cell\u00a0 depletion and sertoli cell depletion in seminiferous tubules. All these findings were prominent in high dose group and progressively increased in severity. Besides this, there was loss of spermatozoa in the lumen of seminiferous tubules in both high and low dose group ,but was profound in high dose group at 12th<\/sup> week. Though cisplatin effectively treats cancer, at an increased dose it has toxic effects to the genitourinary system and other parts of the body.<\/p>\n

Key words:<\/strong> Cisplatin, Rat, Testis, Chemotherapeutic agent, Apoptosis, DNA synthesis<\/p>\n

REFERENCES<\/strong><\/p>\n

    \n
  1. Peyrone, M. Ueber die Einwirkung des Ammonias auf Platinchlor\u00fcr. [On the action of ammonia on platinum chloride]. Ann. Chem. Pharm. 1844;51(1):1-29. https:\/\/doi.org\/10.1002\/jlac.18440510102<\/li>\n
  2. Rosenberg, B. In Nucleic Acid-Metal Ion Interactions; Spiro, T.G., Ed.; John Wiley and Sons, Inc.: New York, NY, USA, 1980;1:1-29.<\/li>\n
  3. Desoize B. Madoulet C. Particular aspects of platinum compounds used at present in cancer treatment. Crit. Rev. Oncol. Hematol. 2002;42:317-325. https:\/\/doi.org\/10.1016\/S1040-8428(01)00219-0<\/li>\n
  4. Wang, Dong, Lippard, Stephen J. Cellular processing of platinum anticancer drugs”. Nature Reviews Drug Discovery. 2005;4(4):307-320. https:\/\/doi.org\/10.1038\/nrd1691 PMid:15789122<\/li>\n
  5. Johnstone TC, Suntharalingam K, Lippard SJ. The next generation of platinum drugs: targeted Pt (II) agents, nanoparticle delivery, and Pt (IV) prodrugs. Chemical reviews. 2016 Mar 9;116(5):3436-86. https:\/\/doi.org\/10.1021\/acs.chemrev.5b00597 PMid:26865551 PMCid:PMC4792284<\/li>\n
  6. Pruefer FG, Lizarraga F, Maldonado V, Melendez-Zajgla J. Participation of Omi Htra2 serine-protease activity in the apoptosis induced by cisplatin on SW480 colon cancer cells. Journal of chemotherapy. 2008 Jun 1;20(3):348-54. https:\/\/doi.org\/10.1179\/joc.2008.20.3.348
    \nPMid:18606591<\/li>\n
  7. Shah N, Dizon D.S. New-generation platinum agents for solid tumors. Future Oncol. 2009;5:33-42. https:\/\/doi.org\/10.2217\/14796694.5.1.33 PMid:19243296<\/li>\n
  8. G\u00fcnes DA, Florea AM, Splettstoesser F, B\u00fcsselberg D. Co-application of arsenic trioxide (As2O3) and cisplatin (CDDP) on human SY-5Y neuroblastoma cells has differential effects on the intracellular calcium concentration ([Ca2+]i) and cytotoxicity. Neurotoxicology 2009;30:194-202. https:\/\/doi.org\/10.1016\/j.neuro.2008.12.001 PMid:19118571<\/li>\n
  9. Flora SJ, Mittal M, Mehta A. Heavy metal induced oxidative stress & its possible reversal by chelation therapy. Indian Journal of Medical Research. 2008 Oct 1;128(4):501.<\/li>\n
  10. Tsang RY, Al-Fayea T, Au HJ. Cisplatin overdose. Drug safety. 2009 Dec 1;32(12):1109-22.
    \nhttps:\/\/doi.org\/10.2165\/11316640-000000000-00000 PMid:19916578<\/li>\n
  11. Vawda AI. Effect of testosterone on cisplatin-induced testicular damage. Arch Androl. Arch Androl 1994;32:53-57. https:\/\/doi.org\/10.3109\/01485019408987767 PMid:8122937<\/li>\n
  12. Aydiner A, Aytekin Y, Topuz E. Effects of Cisplatin on Testicular Tissue and the Leydig Cell-Pituitary Axis. Oncology 1997;54:74-78. https:\/\/doi.org\/10.1159\/000227665 PMid:8978597<\/li>\n
  13. Kadel M, Jha CB, Sangraula H and Bhattacharya SJ. Effects of cisplatin on testes and chromosome of rats: A histological and chromosomal study. J. Pharmacovigilence 2013;1:3.<\/li>\n
  14. HejaziSajjad. Toxicity effect of cisplatin – treatment on rat testis tissues. Annals of Biological Research, 2012; 3(5): 2297-2303.<\/li>\n<\/ol>\n

    \n\t\t\t

    \n \t Cite this article:<\/strong><\/span> Rabiya Amin, Ubaid Ali, Maheen Nazir. EFFECT OF VARIABLE DOSES OF CISPLATIN ON MICROANATOMY OF TESTIS IN ALBINO RATS. Int J Anat Res 2020;8(3.2):7703-7706.\u00a0DOI:\u00a0<\/strong>10.16965\/ijar.2020.190\u00a0 \n <\/div>\t\n\t\t\n","protected":false},"excerpt":{"rendered":"

    Type of Article:\u00a0\u00a0Original Research Volume 8; Issue 3.2 (August 2020) Page No.:\u00a07703-7706 DOI:\u00a0https:\/\/dx.doi.org\/10.16965\/ijar.2020.190 EFFECT OF VARIABLE DOSES OF CISPLATIN ON MICROANATOMY OF TESTIS IN ALBINO RATS Rabiya Amin 1, \u00a0Ubaid Ali *2, Maheen Nazir 3. 1 Senior resident, Department of Anatomy, Dr Chandramma Dayananda Sagar Institute Of Medical Education\u00a0 And Research, Harohalli, Bangalore, Karnataka, India.
    + Read More<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/2337"}],"collection":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/comments?post=2337"}],"version-history":[{"count":1,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/2337\/revisions"}],"predecessor-version":[{"id":2358,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/2337\/revisions\/2358"}],"wp:attachment":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/media?parent=2337"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}