{"id":2253,"date":"2020-05-05T02:59:04","date_gmt":"2020-05-05T02:59:04","guid":{"rendered":"http:\/\/www.ijmhr.org\/IntJAnatRes\/?page_id=2253"},"modified":"2020-05-05T03:15:15","modified_gmt":"2020-05-05T03:15:15","slug":"ijar-2020-125","status":"publish","type":"page","link":"https:\/\/www.ijmhr.org\/IntJAnatRes\/ijar-2020-125","title":{"rendered":"IJAR.2020.125"},"content":{"rendered":"
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Type of Article:<\/strong>\u00a0\u00a0Original Research<\/h3>\n

Volume 8; Issue 2.2 (May 2020)<\/strong><\/h3>\n

Page No.:<\/strong>\u00a07463-7469<\/h3>\n

DOI:\u00a0<\/strong>https:\/\/dx.doi.org\/10.16965\/ijar.2020.125<\/h3>\n

SPECTROSCOPIC, ANAESTHETIC AND HISTOLOGICAL ASSESSMENTS OF VITEX DONIANA<\/em> SWEET ON RETICULAR FORMATION<\/h3>\n

Elizabeth Finbarrs-Bello *1<\/sup>, Oluwatomilayo Patience Ojo 2<\/sup>, Chikezie Jude Okamkpa 1<\/sup>.<\/strong><\/p>\n

*1 <\/sup>Department of Anatomy, Faculty of Basic Medical Sciences. Enugu State University of Science and Technology, Parklane. Enugu State. Nigeria.<\/p>\n

2 <\/sup>Department of Anatomy, Faculty of Basic Medical Sciences, University of Ilorin, Ilorin, Nigeria.<\/p>\n

Corresponding Author: Elizabeth Finbarrs-Bello, <\/strong>Department of Anatomy, Faculty of Basic Medical Sciences. Enugu State University of Science and Technology, Parklane. Enugu State. Nigeria.\u00a0 <\/strong>\u00a0E-Mail:<\/strong> elizabeth.finbarrs-bello@esut.edu.ng.\u00a0 Tel: +2348064113179<\/p>\n

ABSTRACT<\/h3>\n

The study investigated spectroscopic property, anesthetic and histological effects of Vitex donianna<\/em> sweet. Spectroanalysis was conducted using atomic absorption spectroscopy (AAS) and Fourier Transform Infra-Red (FTIR) analysis; anesthetic and histological assessments were carried out on four groups (A, B, C and D) of four rats each. Group A received 0.1ml of physiological saline,\u00a0 group B; 50mg\/kg of ketamine alone (i.m), while groups C and D were administered 200mg\/kg and 400mg\/kg body weight (i.p) of the leave extract respectively 30 minutes later, 50mg\/kg of ketamine was given intramuscularly.\u00a0 Rectal temperature, heart rate and respiratory rate (abdominal beats) were measured.\u00a0\u00a0 The periods of onset and recovery from anaesthesia were recorded. Reticular formation of the brainstem was harvested, manually processed and stained for morphological changes and Nissl granules using H& E and Cresyl fast violet.\u00a0 The AAS and FTIR analysis revealed presence of zinc and functional groups such as ethyl, carboxyl, ether, acetyl and hydroxyl groups which have pharmacological relevant to the extract property.\u00a0 The results showed that administration Vitex donianna <\/em>produced a dose dependent anesthetics effect. The light microscopic study reveal deeply stained nissls granules and few prominently stained pyramidal cells. In conclusion, this could translate into an increased synthesis of serotonin and glutamates neurotransmitters in the reticular formation, which accounts for the anesthetic and reversal of ketamine effects observed in this study.<\/p>\n

Key words<\/strong>: RAS, Vitex Donianna<\/em>, Anesthetic, Histology, Glutamate, Serotonin.<\/p>\n

REFERENCES<\/strong><\/p>\n

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  11. Elizabeth Finbarrs-Bello, J. \u00a0 Nto, I.T.\u00a0 Ikele, M.I.\u00a0 Sani, V.\u00a0 Atuadu.\u00a0 Haematopoietic Enhancing Effect of Ethanolic Seed Extract of Citrullus Lanatus (Watermelon) on Bone Marrow of Wistar Rats. 2016; Ejmp.17 (3):1-7.<\/li>\n
  12. Elizabeth Finbarrs-Bello., Obikili, E. N., Esom, E. A., Anyanwu, G. E Curative effect of aqueous leaf extract of crinum giganteum on NMDA-receptor antagonist-induced schizophrenic Wistar rat model. Open access Macedonian journal of medical sciences. 2016;4(3):337.<\/li>\n<\/ol>\n

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    \n \t Cite this article:<\/strong><\/span> Elizabeth Finbarrs-Bello, Oluwatomilayo Patience Ojo, Chikezie Jude Okamkpa. SPECTROSCOPIC, ANAESTHETIC AND HISTOLOGICAL ASSESSMENTS OF VITEX DONIANA SWEET ON RETICULAR FORMATION. Int J Anat Res 2020;8(2.2):7463-7469.\u00a0DOI:\u00a0<\/strong>10.16965\/ijar.2020.125\u00a0 \n <\/div>\t\n\t\t\n","protected":false},"excerpt":{"rendered":"

    Type of Article:\u00a0\u00a0Original Research Volume 8; Issue 2.2 (May 2020) Page No.:\u00a07463-7469 DOI:\u00a0https:\/\/dx.doi.org\/10.16965\/ijar.2020.125 SPECTROSCOPIC, ANAESTHETIC AND HISTOLOGICAL ASSESSMENTS OF VITEX DONIANA SWEET ON RETICULAR FORMATION Elizabeth Finbarrs-Bello *1, Oluwatomilayo Patience Ojo 2, Chikezie Jude Okamkpa 1. *1 Department of Anatomy, Faculty of Basic Medical Sciences. Enugu State University of Science and Technology, Parklane. Enugu State.
    + Read More<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/2253"}],"collection":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/comments?post=2253"}],"version-history":[{"count":2,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/2253\/revisions"}],"predecessor-version":[{"id":2263,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/2253\/revisions\/2263"}],"wp:attachment":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/media?parent=2253"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}