{"id":1863,"date":"2019-05-05T14:04:48","date_gmt":"2019-05-05T14:04:48","guid":{"rendered":"http:\/\/www.ijmhr.org\/IntJAnatRes\/?page_id=1863"},"modified":"2019-05-05T14:04:48","modified_gmt":"2019-05-05T14:04:48","slug":"ijar-2019-174","status":"publish","type":"page","link":"https:\/\/www.ijmhr.org\/IntJAnatRes\/ijar-2019-174","title":{"rendered":"IJAR.2019.174"},"content":{"rendered":"
<\/i> DOWNLOAD PDF<\/span><\/a><\/div><\/div><\/div>
<\/i> Table of Contents<\/span><\/a><\/div><\/div><\/div><\/div>\n

Type of Article:<\/strong>\u00a0\u00a0Original Research<\/h3>\n

Volume 7; Issue 2.2 (May 2019)<\/strong><\/h3>\n

Page No.:<\/strong>\u00a06581-6589<\/h3>\n

DOI:\u00a0<\/strong>https:\/\/dx.doi.org\/10.16965\/ijar.2019.174<\/h3>\n

ANATOMICAL AND HISTOPATHOLOGICAL EFFECTS OF ZINC OXIDE NANOPARTICLES ON LUNGS IN ADULT MALE ALBINO RATS<\/h3>\n

Nehal Mohamed Nabil *1<\/sup>, Samar El Achy 2<\/sup>.<\/strong><\/p>\n

*1 <\/sup>Anatomy and Embryology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt.<\/p>\n

2<\/sup> Pathology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt.<\/p>\n

Correspondence Author:<\/strong> Nehal Mohamed Nabil, Anatomy and Embryology department, Faculty of Medicine, Alexandria University, Alexandria, Egypt.\u00a0\u00a0E-Mail:<\/strong> nehalnabil895@gmail.com<\/p>\n

ABSTRACT:<\/strong><\/p>\n

Background:<\/strong> Zinc oxide nanoparticles (ZnO-NPs) are frequently used in many fields, including food industry for their antimicrobial activity. Acute exposure to high doses of such particles was found to be toxic to many organs. However, the lung toxicity resulting from chronic exposure to oral doses of ZnO-NPs was not adequately assessed before.<\/p>\n

Aim of the work: <\/strong>to detect the anatomical and histopathological effects of chronic exposure to ingested ZnO-NPs on the lung of normal adult male albino rat.<\/p>\n

Material and methods:<\/strong> It was carried out on 30 adult male Swiss albino rats with an average weight of 150-200 gm. They were divided into two groups: Group I: 10 rats serving as control group; Group II: 20 rats serving as experimental groups, divided into 2 subgroups (a&b) receiving oral ingestion by orogastric tube of a single daily dose (125mg\/ kg) of average 20 nm sized ZnO-NPs for different durations: Group IIa (n=10): \u00a0for 120 days; Group IIb (n=10): for 180 days. Histopathology and immunohistochemistry of the lungs in the three groups was performed to detect the possible effect of such exposure.<\/p>\n

Results:<\/strong> Oral administration of ZnO-NPs induced lung damage manifested by congested blood vessels, interstitial inflammation, infiltration with macrophages& lymphocytes, supurative granuloma, thickened interalveolar septa. These changes were more evident with longer exposure for 180 days (P\u22640.5). This substantial damage to the lungs is caused by oxidative stress and chronic inflammation.<\/p>\n

Conclusion: <\/strong>Caution should be considered when using these particles in food packaging and food additives, and for those who are in close contact with these particles especially in factories.<\/p>\n

Key words:<\/strong> ZnO-NPs, Oral ingestion, Immunohistochemistry, lungs.<\/p>\n

REFERENCES<\/strong><\/p>\n

    \n
  1. Rashidi L, Khosravi-Darani K. The applications of nanotechnology in food industry. Crit Rev Food Sci Nutr 2011; 51(8):723\u2013730.<\/li>\n
  2. Clarence SY, Geoffrey SS, Sunny EI. Nanoparticles toxicity and their routes of exposure. Pak J Pharm Sci 2012; 25(2):477\u2013491.<\/li>\n
  3. Matsuyama\u00a0K,\u00a0Ihsan\u00a0N,\u00a0Irie\u00a0K,\u00a0Mishima\u00a0K,\u00a0Okuyama T,\u00a0Muto H. Bioimaging\u00a0application\u00a0of\u00a0highly\u00a0luminescent\u00a0silica-coated\u00a0ZnOnanoparticle\u00a0quantum dots\u00a0with\u00a0biotin. J\u00a0Colloid\u00a0Interface\u00a0Sci \u00a02013; 399:19-25.<\/li>\n
  4. Jin T, Sun D, Su JY, Zhang H, Sue HJ. Antimicrobial efficacy of zinc oxide quantum dots against Listeria monocytogenes, Salmonella Enteritidis, and Escherichia coli O157:H7. J Food Sci 2009; 74(1): M46\u2013M52.<\/li>\n
  5. Lu, S.; Zhang, W.; Zhang, R.; Liu, P.; Wang, Q.; Shang, Y.; Wu, M.; Donaldson, K.; Wang, Q. Comparison of cellular toxicity caused by ambient ultrafine particles and engineered metal oxide nanoparticles. Part Fibre Toxicol 2015; 12 :5 .<\/li>\n
  6. Sharma V, Singh P, Pandey AK, Dhawan A. Induction of oxidative stress, DNA damage and apoptosis in mouse liver after sub-acute oral exposure to zinc oxide nanoparticles. Mutat Res 2012; 745: 84-91.<\/li>\n
  7. Vandebriel RJ and De Jong WH. A review of mammalian toxicity of ZnO nanoparticles. Nanotechnol. Sci. Applic\u00a0 2012; 5: 61-71.<\/li>\n
  8. Ghorbani M., Soheili S. Moradhaseli S, and Shokouhian A. Histopathological effects of ZnO nanoparticles on liver and heart tissues in wistar rats. Adv. Biores 2013; 4: 83-88.<\/li>\n
  9. [9].\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Park HS,\u00a0Kim SJ,\u00a0Lee TJ,\u00a0Kim GY,\u00a0Meang E,\u00a0Hong JS,\u00a0et al. A\u00a090-day\u00a0study\u00a0of\u00a0sub \u00a0chronic\u00a0oral\u00a0toxicity\u00a0of\u00a020\u00a0nm\u00a0positively\u00a0charged\u00a0zinc\u00a0oxide\u00a0nanoparticles in\u00a0Sprague\u00a0Dawley\u00a0rats. Int J Nanomedicine \u00a02014; 9 (Suppl 2): 93\u2013107.<\/li>\n
  10. Ko JW,\u00a0Hong ET,\u00a0Lee IC,\u00a0Park SH,\u00a0Park JI,\u00a0Seong NW, et al. Evaluation of 2-week\u00a0repeated\u00a0oral\u00a0dose\u00a0toxicity\u00a0of 100 nm\u00a0zinc oxide\u00a0nanoparticles\u00a0in rats. Lab Anim Res \u00a02015; 31(3):139-47.<\/li>\n
  11. Cho WS,\u00a0Duffin R,\u00a0Howie SE,\u00a0Scotton CJ,\u00a0Wallace WA,\u00a0Macnee W,\u00a0et al. Progressive\u00a0severe\u00a0lung\u00a0injury\u00a0by\u00a0zinc\u00a0oxide\u00a0nanoparticles;the\u00a0role\u00a0of\u00a0Zn2+\u00a0dissolution inside lysosomes. Part Fibre Toxicol \u00a02011; 8: 27.<\/li>\n
  12. Shokouhian A, Soheili S, Moradhaseli S, Fazli L, Ardestani MS, and Masoud Ghorbani . Toxicity of zinc oxide nanoparticles in lung tissue after repeated oral administration. American Journal of Pharmacology and Toxicology 2013; 8 (4): 148-154.<\/li>\n
  13. Jain S,\u00a0Rachamalla M,\u00a0Kulkarni A,\u00a0Kaur J,\u00a0Tikoo K. Pulmonary fibrotic response to inhalation of ZnO nanoparticles and toluene co-exposure through directed flow nose only exposure chamber. Inhal Toxicol.\u00a02013 ; 25(13):703-713.<\/li>\n
  14. Morimoto Y,\u00a0Izumi H,\u00a0Yoshiura Y,\u00a0Tomonaga T,\u00a0Oyabu T,\u00a0Myojo T,\u00a0et al. Evaluation\u00a0of\u00a0Pulmonary\u00a0Toxicity\u00a0of\u00a0ZincOxide\u00a0Nanoparticles\u00a0Following\u00a0Inhalation\u00a0and Intratracheal\u00a0Instillation. Int J Mol Sci \u00a02016; 17(8): E1241.<\/li>\n
  15. Yoo J, Seo GB. , Yoon BI. , Lim YM, Kim P, Kim HM , Kwon JT. Evaluation of recovery from acute lung injury induced by intratracheal instillation of zinc oxide nanoparticles. Applied ecology and environmental research 2018; 16(3):3145-3157.<\/li>\n
  16. Mahato TH, Prasad GK , Singh BJ, Acharya J, Srivastava AR, Vijayaraghavan R. Nanocrystalline zinc oxide for the decontamination of sarin. Journal of Hazardous Materials 2009; 165: 928\u2013932.<\/li>\n
  17. Suryanarayana C, Norton M G. X- ray Diffraction: A practical approach (Artech House Telecommunications) New York: Plenum Press; 1998. P. 63-96.<\/li>\n
  18. Waseda Y, Muramatsu A. Morphology control of materials and nanoparticles: Advanced materials processing and characterization. Berlin: Springer; 2004. P. 85-87.<\/li>\n
  19. Eidi H , Joubert O, Nemos C, Grandemange S, Mograbi B, Foliguet B, et al. Drug delivery by polymeric nanoparticles induces autophagy in macrophages. Int J Pharm 2012; 422 (1): 495-503.<\/li>\n
  20. Hosokawa M, Nogi K, Naito M, Yokoyama T. Nanoparticle technology hand book. 2nd ed. Amesterdam: Elsevier; 2012, P. 5-51.<\/li>\n
  21. El Morshedi N, AlZahrani I, Kizilbash NA, AlFayez HA. Toxic effect of Zinc Oxide Nanoparticles on some organs in Experimental Male Wister Rats. IJAR 2014; 2 (4): 907-915.<\/li>\n
  22. Chung HE, Yu J , Baek M, Lee JA, Kim MS, Kim SH, et al. Toxicokinetics of zinc oxide nanoparticles in rats. J Phs Conf Ser \u00a02013; 429; 012037.<\/li>\n
  23. Bai D P, Zhang X F, Zhang G L, Huang Y F, and Gurunathan S. Zinc oxide nanoparticles induce apoptosis and autophagy in human ovarian cancer cells. Int. J. Nanomed 2017;12: 6521\u20136535.<\/li>\n
  24. Fukui H,\u00a0Iwahashi H,\u00a0Nishio K,\u00a0Hagihara Y,\u00a0Yoshida Y,\u00a0Horie M. Ascorbic acid prevents\u00a0zinc oxide\u00a0nanoparticle-induced\u00a0intracellular oxidative stress and inflammatory responses. Toxicol Ind Health \u00a02017; 33(9):687-695.<\/li>\n
  25. Byers DE, and Holtzman MJ. Alternatively activated macrophages and airway disease. Chest 2011; 140:768\u2013774.<\/li>\n
  26. Nemmar A,\u00a0Al-Salam S,\u00a0Beegam S,\u00a0Yuvaraju P,\u00a0Ali BH. The\u00a0acute\u00a0pulmonary\u00a0and\u00a0thrombotic\u00a0effects\u00a0of\u00a0cerium oxide\u00a0nanoparticles\u00a0after\u00a0intra-tracheal instillation\u00a0in\u00a0mice. Int J Nanomedicine.\u00a02017; 12: 2913- 2922.<\/li>\n
  27. Qiao Y, Liang X, Yan Y, Lu Y, Zhang D, Yao W, et al. Identification of Exosomal miRNAs in Rats With Pulmonary Neutrophilic Inflammation Induced by Zinc Oxide Nanoparticles. \u00a0 Physiol\u00a0 2018; 9:217.<\/li>\n<\/ol>\n

    \n\t\t\t

    \n \t Cite this article:<\/strong><\/span> Nehal Mohamed Nabil, Samar El Achy. ANATOMICAL AND HISTOPATHOLOGICAL EFFECTS OF ZINC OXIDE NANOPARTICLES ON LUNGS IN ADULT MALE ALBINO RATS. Int J Anat Res 2019;7(2.2):6581-6589.\u00a0DOI:\u00a0<\/strong>10.16965\/ijar.2019.174\u00a0 \n <\/div>\t\n\t\t\n","protected":false},"excerpt":{"rendered":"

    Type of Article:\u00a0\u00a0Original Research Volume 7; Issue 2.2 (May 2019) Page No.:\u00a06581-6589 DOI:\u00a0https:\/\/dx.doi.org\/10.16965\/ijar.2019.174 ANATOMICAL AND HISTOPATHOLOGICAL EFFECTS OF ZINC OXIDE NANOPARTICLES ON LUNGS IN ADULT MALE ALBINO RATS Nehal Mohamed Nabil *1, Samar El Achy 2. *1 Anatomy and Embryology Department, Faculty of Medicine, Alexandria University, Alexandria, Egypt. 2 Pathology Department, Faculty of Medicine, Alexandria
    + Read More<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/1863"}],"collection":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/comments?post=1863"}],"version-history":[{"count":1,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/1863\/revisions"}],"predecessor-version":[{"id":1892,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/1863\/revisions\/1892"}],"wp:attachment":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/media?parent=1863"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}