{"id":1469,"date":"2018-09-05T13:41:55","date_gmt":"2018-09-05T13:41:55","guid":{"rendered":"http:\/\/www.ijmhr.org\/IntJAnatRes\/?page_id=1469"},"modified":"2018-09-05T13:41:55","modified_gmt":"2018-09-05T13:41:55","slug":"ijar-2018-220","status":"publish","type":"page","link":"https:\/\/www.ijmhr.org\/IntJAnatRes\/ijar-2018-220","title":{"rendered":"IJAR.2018.220"},"content":{"rendered":"<div class=\"su-row\"><div class=\"su-column su-column-size-1-2\"><div class=\"su-column-inner su-u-clearfix su-u-trim\"><div class=\"su-button-center\"><a href=\"https:\/\/www.ijmhr.org\/ijar.6.3\/IJAR.2018.220.pdf\" class=\"su-button su-button-style-default\" style=\"color:#FFFFFF;background-color:#6b0e00;border-color:#560c00;border-radius:5px;-moz-border-radius:5px;-webkit-border-radius:5px\" target=\"_self\"><span style=\"color:#FFFFFF;padding:6px 16px;font-size:13px;line-height:20px;border-color:#98574d;border-radius:5px;-moz-border-radius:5px;-webkit-border-radius:5px;text-shadow:none;-moz-text-shadow:none;-webkit-text-shadow:none\"><i class=\"sui sui-cloud-download\" style=\"font-size:13px;color:#FFFFFF\"><\/i> DOWNLOAD PDF<\/span><\/a><\/div><\/div><\/div> <div class=\"su-column su-column-size-1-2\"><div class=\"su-column-inner su-u-clearfix su-u-trim\"><div class=\"su-button-center\"><a href=\"https:\/\/ijmhr.org\/ijar-vol-6-3.htm\" class=\"su-button su-button-style-default\" style=\"color:#FFFFFF;background-color:#6b0e00;border-color:#560c00;border-radius:5px;-moz-border-radius:5px;-webkit-border-radius:5px\" target=\"_self\"><span style=\"color:#FFFFFF;padding:6px 16px;font-size:13px;line-height:20px;border-color:#98574d;border-radius:5px;-moz-border-radius:5px;-webkit-border-radius:5px;text-shadow:none;-moz-text-shadow:none;-webkit-text-shadow:none\"><i class=\"sui sui-book\" style=\"font-size:13px;color:#FFFFFF\"><\/i> Table of Contents<\/span><\/a><\/div><\/div><\/div><\/div>\n<h3 style=\"text-align: justify;\"><strong>Type of Article:<\/strong>\u00a0\u00a0Original Research<\/h3>\n<h3 style=\"text-align: justify;\"><strong>Volume 6; Issue 3.3 (Septmber 2018)<\/strong><\/h3>\n<h3 style=\"text-align: justify;\"><strong>Page No.:<\/strong>\u00a05593-5596<\/h3>\n<h3 style=\"text-align: justify;\"><strong>DOI:\u00a0<\/strong>https:\/\/dx.doi.org\/10.16965\/ijar.2018.220<\/h3>\n<h3 style=\"text-align: justify;\">BUCCAL MUCOSAL DNA DAMAGE FOLLOWING RADIOTHERAPY IN PATIENTS WITH HEAD AND NECK CANCER<\/h3>\n<p style=\"text-align: justify;\"><strong>Vinay Kumar V <sup>1<\/sup>, Sachin KS *<sup>2<\/sup>, Vishak R <sup>3<\/sup>.<\/strong><\/p>\n<p><sup>*1<\/sup> Professor, Department of Anatomy, K S Hegde Medical Academy, Deralakatte, Mangaluru, Karnataka, India.<strong><br \/>\n<\/strong><sup>2 <\/sup>Assistant Professor, Department of Anatomy, K S Hegde Medical Academy, Deralakatte, Mangaluru, Karnataka, India.<\/p>\n<p><sup>3<\/sup> Department of Anatomy, K S Hegde Medical Academy, Deralakatte, Mangaluru, Karnataka, India.<\/p>\n<p style=\"text-align: justify;\"><strong>Address for Correspondence: <\/strong>Dr. Sachin KS, Assistant Professor, Department of Anatomy, K S Hegde Medical Academy, Deralakatte, Mangaluru- 575018. Karnataka, India. PH 7795767676.\u00a0<strong>E-Mail: \u00a0 <\/strong>dr7795767676@gmail.com<\/p>\n<p style=\"text-align: justify;\"><strong>ABSTRACT:<\/strong><\/p>\n<p style=\"text-align: justify;\">Patients with head and neck cancer receiving radiotherapy in addition to antitumour effects also are exposed to ionizing radiation which may also damage normal tissue located in the field of radiation. The study was done on buccal scrapings in patients with head and neck cancer patients receiving radiotherapy at the hospital.\u00a0 The present study aims at determining the changes in MN in the buccal smears in pre and post RT in patients with head and neck cancer patients. The mean number of MN was significantly high in buccal scrapings of patients post RT suggesting DNA damage following exposure to radiations during RT.<\/p>\n<p style=\"text-align: justify;\"><strong>Key Words:<\/strong> Head and neck cancer, Radiotherapy, Micronucleus assay, Buccal mucosa.<\/p>\n<p style=\"text-align: justify;\"><strong>REFERENCES<\/strong><\/p>\n<ol>\n<li style=\"text-align: justify;\">Mitra D, Malkoski SP, Wang XJ. Cancer stem cells in head and neck cancer. Cancers (Basel) 2011; 3: 415-427.<\/li>\n<li style=\"text-align: justify;\">Argiris A, Karamouzis MV, Raben D, Ferris RL. Head and neck cancer. 2008; 371: 1695-1709.<\/li>\n<li style=\"text-align: justify;\">Jemal A, Center MM, Ward E, Thun MJ. Cancer occurrence. Methods Mol Biol. 2009; 471: 3-29.<\/li>\n<li style=\"text-align: justify;\">Ferlay J, Shin HR, Bray F, Forman D, Mathers C, Parkin DM. Estimates of worldwide burden of cancer in 2008: GLOBOCAN. 2008. Int J Cancer. 2010; 127: 2893-2917.<\/li>\n<li style=\"text-align: justify;\">Pulte D, Brenner H. Changes in survival in head and neck cancers in the late 20th and early 21st century: a period analysis. 2010; 15: 994-1001.<\/li>\n<li style=\"text-align: justify;\">Dobbs J, Barret A, Ash D. Practical radiotherpy planing. 3<sup>rd<\/sup> Ed, London, Arnold: Oxford university press\u037e 1999.<\/li>\n<li style=\"text-align: justify;\">Handshel J, Prott FJ, Sunderkotte C, Metze D, Meyer U, Joos U. Irradiation induces increase of adhesion molecules and accumulation of beta2 integrin expressing cells in humans. Int J Radiat Onco Biol Phys. 1999; 45: 475-81.<\/li>\n<li style=\"text-align: justify;\">Taylor SE, Miller EG. Preemptive pharmacologic intervention in the radiation induced salivary dysfunction. Proc Soc Exp Biol Med. 1999; 221: 1426.<\/li>\n<li style=\"text-align: justify;\">Nagler RM. The enigmatic mechanisms of irradiation induced damage to major salivary glands. Oral Dis. 2002; 8: 1416.<\/li>\n<li style=\"text-align: justify;\">Spielman AI. Chemosensory function and dysfunction. Crit Rev Oral Bio Med. 1998; 9: 267-91.<\/li>\n<li style=\"text-align: justify;\">Anneroth G, Holm LE, Karlsson G. The effect of radiation on teeth: A clinical, histological, and microradiographic study. Int J oral Surgey. 1985\u037e 14: 269-74.<\/li>\n<li style=\"text-align: justify;\">Steel CG. Basic clinical radiobiology. 3<sup>rd<\/sup> edition, London, Arnold: Oxford university press\u037e 2002: 231-40.<\/li>\n<li style=\"text-align: justify;\">Hall EJ. Radiobiology for the radiobiologist. 5 th Ed, Philadelphia: Lippincoat Willams and Wilkins\u037e 2000: 453-6.<\/li>\n<li style=\"text-align: justify;\">Yosuf ZW, Bakri MM. Severe progressive periodontal destruction due to radiation tissue injury. J Periodontol. 1993; 64: 125-38.<\/li>\n<li style=\"text-align: justify;\">Epstein JB, Lunn R, Le N, StevensonMooreP. Periodontal attachment loss in patients after head and neck radiation therapy. Oral Surg Oral Med Oral Pathol Endod. 1998; 86: 673-7.<\/li>\n<li style=\"text-align: justify;\">Engels H, Wambersie A. Relative biological effectiveness of neutrons for cancer induction and other late effects: a review of biological data recent. Recent Results Cancer Res. 1998; 150: 54-87.<\/li>\n<li style=\"text-align: justify;\">Kaiser HE, Nasir A, Groger A, M., Link, C., J., Jr. The etiology of second primary neoplasms. In vivo. 1998; 12: 89-93.<\/li>\n<li style=\"text-align: justify;\">Perel Y, Leverger G, Carrere A, Caudry M, Garabedian EN, Ansoborlo S, Vergnes P. Second thyroid neoplasms after prophylactic cranial irradiation for acute lymphoblastic leukemia. Am J. Hamatol. 1998; 59: 91-94.<\/li>\n<li style=\"text-align: justify;\">Sarto F, Finotto S, Giacomelli L, Mazzotti D, Tomanin R, Levis AG. The micronucleus assay in exfoliated cells of the human buccal. Mutagenesis. 1987; 2: 11-17.<\/li>\n<li style=\"text-align: justify;\">Fenech M, Morley A. Solutions to the kinetic problem in the micronucleus assay. Cytobios 1985; 43: 233-246.<\/li>\n<li style=\"text-align: justify;\">Tolbert P, Shy CM, Allen JW. Micronucleus and other nuclear anomalies in buccal smears: Methods development.Mut Res.1992; 271: 69-77.<\/li>\n<li style=\"text-align: justify;\">Fenech M, Morley AA. Measurements of micronuclei in lymphocytes. Mutat. Res. 1985. 147: 29-36.<\/li>\n<li style=\"text-align: justify;\">Casartelli G, Bonatti S, De Ferrari M, Scala M, Mereu P, Margarino G, et al. Micronucleus<\/li>\n<li style=\"text-align: justify;\">frequencies in exfoliated buccal cells in normal mucosa, precancerous lesions and squamous cell<\/li>\n<li style=\"text-align: justify;\">Anal Quant Cytol Histol. 2000\u037e 22: 486-92.<\/li>\n<li style=\"text-align: justify;\">Stich HF, San RH, Rosin MP. Adaptation of the DNA repair and micronucleus tests to human cell suspensions and exfoliated cells. Ann N Y Acad Sci.1983\u037e 407: 93-105.<\/li>\n<li style=\"text-align: justify;\">Sarto F, Finotto S, Giacomelli L, Mazzotti D, Tomanin R, Levis AG. The micronucleus assay in exfoliated cells of the human buccal mucosa. Mutagenesis. 1987\u037e 2: 11-17.<\/li>\n<li style=\"text-align: justify;\">Moore LE, Warner ML, Smith AH, Kalman D, Smith MT. Use of the fluorescent micronucleus assay to detect the genotoxic effects of radiation and arsenic exposure in exfoliated human epithelial cells. Environ Mol Mutagen. 1996\u037e 27:176-84.<\/li>\n<li style=\"text-align: justify;\">Bina K, Padala SR. Micronuclei assay of exfoliated oral buccal cells: Means to assess the nuclear abnormalities in different diseases. Journal of Cancer Research and Therapeutics. 8 (2). 2012: 184-191.<\/li>\n<\/ol>\n<p style=\"text-align: justify;\">\n\t\t\t <div class=\"promo1\" style=\"background-color:#f7f7f7; border-color: #6b0e00 #e8e6e6 #e8e6e6;\">\n             \t <span style=\"color: #800000;\"><strong>Cite this article:<\/strong><\/span> Vinay Kumar V, Sachin KS, Vishak R. BUCCAL MUCOSAL DNA DAMAGE FOLLOWING RADIOTHERAPY IN PATIENTS WITH HEAD AND NECK CANCER. Int J Anat Res 2018;6(3.3):5593-5596.\u00a0<strong>DOI:\u00a0<\/strong>10.16965\/ijar.2018.220\u00a0 \n             <\/div>\t\n\t\t\n","protected":false},"excerpt":{"rendered":"<p>Type of Article:\u00a0\u00a0Original Research Volume 6; Issue 3.3 (Septmber 2018) Page No.:\u00a05593-5596 DOI:\u00a0https:\/\/dx.doi.org\/10.16965\/ijar.2018.220 BUCCAL MUCOSAL DNA DAMAGE FOLLOWING RADIOTHERAPY IN PATIENTS WITH HEAD AND NECK CANCER Vinay Kumar V 1, Sachin KS *2, Vishak R 3. *1 Professor, Department of Anatomy, K S Hegde Medical Academy, Deralakatte, Mangaluru, Karnataka, India. 2 Assistant Professor, Department of<br \/><a class=\"moretag\" href=\"https:\/\/www.ijmhr.org\/IntJAnatRes\/ijar-2018-220\">+ Read More<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/1469"}],"collection":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/comments?post=1469"}],"version-history":[{"count":1,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/1469\/revisions"}],"predecessor-version":[{"id":1470,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/1469\/revisions\/1470"}],"wp:attachment":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/media?parent=1469"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}