{"id":1252,"date":"2018-04-05T13:37:31","date_gmt":"2018-04-05T13:37:31","guid":{"rendered":"http:\/\/www.ijmhr.org\/IntJAnatRes\/?page_id=1252"},"modified":"2018-04-05T13:49:04","modified_gmt":"2018-04-05T13:49:04","slug":"ijar-2018-115","status":"publish","type":"page","link":"https:\/\/www.ijmhr.org\/IntJAnatRes\/ijar-2018-115","title":{"rendered":"IJAR.2018.115"},"content":{"rendered":"
<\/i> DOWNLOAD PDF<\/span><\/a><\/div><\/div><\/div>
<\/i> Table of Contents<\/span><\/a><\/div><\/div><\/div><\/div>\n

Type of Article:<\/strong>\u00a0\u00a0Original Research<\/h3>\n

Volume 6; Issue 2.1 (April 2018)<\/strong><\/h3>\n

Page No.:<\/strong>\u00a05079-5082<\/h3>\n

DOI:\u00a0<\/strong>https:\/\/dx.doi.org\/10.16965\/ijar.2018.115<\/h3>\n

GENDER DETERMINATION USING PERIPHERAL BLOOD SMEAR <\/strong><\/h3>\n

Shalini Gopal *1<\/sup>, Rashmi Naik 2<\/sup>, Ahmed Mujib B.R 3<\/sup>, Arun Kumar. N 4<\/sup>.<\/strong><\/p>\n

*1<\/sup> BDS, Post Graduate Student, Department of Oral Pathology and Microbiology, Bapuji Dental College and Hospital, Davangere-577004, Karnataka, India.<\/p>\n

2<\/sup> MDS, Reader, Department Of Oral Pathology and Microbiology, Bapuji Dental College and Hospital, Davangere-577004, Karnataka, India.<\/p>\n

3<\/sup> MDS, Professor and Head of the Department, Department of Oral Pathology and Microbiology, Bapuji Dental College and Hospital, Davangere-577004, Karnataka, India.<\/p>\n

4 <\/sup>PhD, Biostatistician, Bapuji Dental College And Hospital, Davangere-577004, Karnataka, India.<\/p>\n

Corresponding author:<\/strong> Dr. Shalini. G, BDS, Post Graduate Student, Department of Oral Pathology and Microbiology, Bapuji Dental College and Hospital, Davangere-577004, Karnataka, India. Ph no: +919731839314 E-Mail:<\/strong> shalinichinnu82@yahoo.com<\/p>\n

ABSTRACT:<\/strong><\/p>\n

Background<\/strong>: Sex chromatin is a chromatin mass of 1 micron size usually seen at the periphery of nucleus in females. The term sex chromatin comprises of two superficially dissimilar structures known as Barr body in epithelial cells, other tissue cells and as Drumstick appearance in polymorphonuclear leucocytes. Aim of the study is gender determination by drumstick appearance of neutrophils using Leishman\u2019s stain and Field stain.<\/p>\n

Materials and Methods:<\/strong> The present study included 60 individuals ranging from 20-50 age group of both the sexes to observe the morphology of sex chromatin in neutrophil by using Leishman\u2019s and Field stain for the gender determination and comparison between the 2 staining procedure, using research microscope.<\/p>\n

Results<\/strong>: Presence of drumsticks, sessile nodule and non specific appendages like racket, tag, hook, minor lobe, small club, were found in neutrophils. In males 37% of drumstick, 7% of sessile nodule, 20% of racket structure, 3% small club, 13% minor lobe, 7% hook, 3% tag, was seen and in females 10% of drumstick, 60% of sessile nodule, 17% of racket structure, 7% small club, 1% minor lobe, 0% hook, 3% tag, was seen and field\u2019s stain showed better results in appreciating the morphology of neutrophil.<\/p>\n

Conclusion<\/strong>: Observation on morphology and comparison between 2 different stain presented a moderate agreement in gender determination.<\/p>\n

KEY WORDS<\/strong>: Sex chromatin, drumstick, sessile nodule, non specific appendages.<\/p>\n

REFERENCES<\/strong><\/p>\n

    \n
  1. Davidson W.M, Smith D.R. A morphological sex difference in the polymorphonuclear neutrophil leucocytes. Brit Med J 1954;2:6.<\/li>\n
  2. Miles CP. Peripheral Position of Sex Chromatin. Nature 1961; 191: 626 \u2013 627.<\/li>\n
  3. Levine H. \u2018Clinical Cytogenetics\u2019. 1971; pp. 77-81. Churchill: London.<\/li>\n
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  5. Briggs D.K. The individuality of nuclear chromatin with particular reference to polymorphonuclear neutrophil leukocytes. Blood 1958;13:986-1000.<\/li>\n
  6. Tomonaga M, Matsuura G,Watanabe B, Kamochi Y, Ozono N. Leukocyte drumsticks in chronic granulocytic leukemia and related disorders. Blood 1961;18:581-591.<\/li>\n
  7. Mittwoch U. Frequency of drumsticks in normal women and in patients with chromosomal abnormalities. Nature 1964;201;317-319.<\/li>\n
  8. Jacobs PA, Baikie AG, Court BrownW.M., MacGregor TN,Maclean N, Harnden DG. Lancet,1959; ii: 423.<\/li>\n
  9. Wondergem MJ, Ossenkoppele GJ. Genotyping by morphology. Blood 2011; 117:2566.<\/li>\n
  10. Kesaree N,Woolley PV Jr. A phenotypic female with 49 chromosomes, presumably. A case report. J Pediatr. 1963;63:1099-1103.<\/li>\n
  11. Shankar,P.Brahmaji Master, L.C.Obulesu, Y.K.C.Rangiah. A Study of Sex Cheromatin Form Buccal Smear. Journal of Evolution of Medical and Dental Sciences 2015; 4(83):14497-14503.<\/li>\n
  12. Reddy AVS, Prakash AR, Killampalli LK, Rajinikanth M, Sreenath G, Sabiha PB. Gender determination using barr bodies from teeth exposed to high temperatures.\u00a0Journal of Forensic Dental Sciences. 2017;9(1):44.<\/li>\n<\/ol>\n

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    \n \t Cite this article:<\/strong><\/span> Shalini Gopal, Rashmi Naik, Ahmed Mujib B.R, Arun Kumar. N. GENDER DETERMINATION USING PERIPHERAL BLOOD SMEAR. Int J Anat Res 2018;6(2.1):5079-5082.\u00a0DOI:\u00a0<\/strong>10.16965\/ijar.2018.115\u00a0 \n <\/div>\t\n\t\t\n","protected":false},"excerpt":{"rendered":"

    Type of Article:\u00a0\u00a0Original Research Volume 6; Issue 2.1 (April 2018) Page No.:\u00a05079-5082 DOI:\u00a0https:\/\/dx.doi.org\/10.16965\/ijar.2018.115 GENDER DETERMINATION USING PERIPHERAL BLOOD SMEAR Shalini Gopal *1, Rashmi Naik 2, Ahmed Mujib B.R 3, Arun Kumar. N 4. *1 BDS, Post Graduate Student, Department of Oral Pathology and Microbiology, Bapuji Dental College and Hospital, Davangere-577004, Karnataka, India. 2 MDS, Reader,
    + Read More<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/1252"}],"collection":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/comments?post=1252"}],"version-history":[{"count":3,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/1252\/revisions"}],"predecessor-version":[{"id":1260,"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/pages\/1252\/revisions\/1260"}],"wp:attachment":[{"href":"https:\/\/www.ijmhr.org\/IntJAnatRes\/wp-json\/wp\/v2\/media?parent=1252"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}